Progress and challenges in sensing of mycotoxins using molecularly imprinted polymers

Hua, Yongbiao; Ahmadi, Younes; Sonne, Christian; Kim, Ki‐Hyun

doi:10.1016/j.envpol.2022.119218

Cited by 29 publications

(8 citation statements)

References 162 publications

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“…The first step was selecting the best conditions for MIP adsorption; to this end, the solvents used in the different steps and the amount of polymer were both optimized. The ‘solvent’ used in the loading is crucial for the adsorption of the analyte by MIPs; in accordance with the literature, water resulted in the best solvent for AFs adsorption [ 1 , 6 ]. The maximum adsorption was reached using 5 mg of polymer in the 1 mL SPE cartridges.…”

Section: Resultssupporting

confidence: 78%

“…Aflatoxins (AFs) are a group of compounds having as basic structure a coumarin and a double-furan ring and are produced as secondary metabolites by fungi of Aspergillus family as A. flavus, A. parasiticus, and A. nomius [ 1 , 2 , 3 ]. AFs can contaminate foodstuffs such as cereals, nuts, fruits, dried fruits, and oilseeds in field (harvest period) or during drying/storage [ 4 , 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

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Study on Molecularly Imprinted Polymers Obtained Sonochemically for the Determination of Aflatoxins in Food

Palmieri

Elfadil²,

Fanti³

et al. 2023

Molecules

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Aflatoxins (AFs) are fungi secondary metabolites produced by the Aspergillus family. These compounds can enter the food chain through food contamination, representing a risk to human health. Commercial immunoaffinity columns are widely used for the extraction and cleanup of AFs from food samples; however, their high cost and large solvent consumption create a need for alternative strategies. In this work, an alternative strategy for producing molecularly imprinted polymers (MIPs) was proposed to extract aflatoxins AFB1, AFB2, AFG1, and AFG2 from complex food samples, using liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS). The MIPs were synthesized via a low-cost and rapid (5 min) sonochemical free-radical polymerization, using 1-hydroxy-2-naphthoic acid as a dummy template. MIPs-based solid phase extraction performance was tested on 17 dietary supplements (vegetables, fruits, and cereals), obtaining appreciable recovery rates (65–90%) and good reproducibility (RSD ≤ 6%, n = 3); the selectivity towards other mycotoxins was proved and the data obtained compared with commercial immunoaffinity columns. The proposed strategy can be considered an alternative affordable approach to the classical immunoaffinity columns, since it is more selective and better performing.

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Section: Resultssupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Study on Molecularly Imprinted Polymers Obtained Sonochemically for the Determination of Aflatoxins in Food

Palmieri

Elfadil²,

Fanti³

et al. 2023

Molecules

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show abstract

“…For example, using molecular imprinting technology to specifically recognize template molecules and preparing specific recognition elements have been demonstrated. [105][106][107] Furthermore, it is important to fully test promising nanomaterials in real samples. Finally, an interesting idea is to combine sensing nanomaterials with bioprobes for the same target to see if even better selectivity can be achieved.…”

Section: Summary and Future Perspectivesmentioning

confidence: 99%

Nanomaterials for molecular recognition: specific adsorption and regulation of nanozyme activities

Wang

et al. 2023

Mater. Chem. Front.

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“…Aspergillus flavus, Aspergillus parasiticus, and Aspergillus oryzae are the three primary sources of aflatoxin, a mycotoxin that is both immunosuppressive and carcinogenic and has detrimental effects on both humans and animals. , Mycotoxins pose a significant hazard to food safety since they are challenging to completely eliminate during food processing due to the peculiar behaviors of some strains of bacteria. Aflatoxin B 1 (AFB 1 ), in particular, is regarded as the most hazardous substance among the aflatoxins because of its significant mutagenicity and carcinogenicity .…”

Section: Introductionmentioning

confidence: 99%

Microelectromechanical Microsystems-Supported Photothermal Immunoassay for Point-of-Care Testing of Aflatoxin B1 in Foodstuff

et al. 2023

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Accurate identification of acutely toxic and low-fatality mycotoxins on a large scale in a quick and cheap manner is critical for reducing population mortality. Herein, a portable photothermal immunosensing platform supported by a microelectromechanical microsystem (MEMS) without enzyme involvement was reported for point-of-care testing of mycotoxins (in the case of aflatoxin B 1 , AFB 1 ) in food based on the precise satellite structure of Au nanoparticles. The synthesized Au nanoparticles with a well-defined, graded satellite structure exhibited a significantly enhanced photothermal response and were coupled by AFB 1 antibodies to form signal conversion probes by physisorption for further target-promoted competitive responses in microplates. In addition, a coin-sized miniature NIR camera device was constructed for temperature acquisition during target testing based on advanced MEMS fabrication technology to address the limitation of expensive signal acquisition components of current photothermal sensors. The proposed MEMS readoutbased microphotothermal test method provides excellent AFB 1 response in the range of 0.5−500 ng g −1 with detection limits as low as 0.27 ng g −1 . In addition, the main reasons for the efficient photothermal transduction efficiency of Au with different graded structures were analyzed by finite element simulations, providing theoretical guidance for the development of new Au-based photothermal agents. In conclusion, the proposed portable micro-photothermal test system offers great potential for point-of-care diagnostics for residents, which will continue to facilitate immediate food safety identification in resource-limited regions.

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Progress and challenges in sensing of mycotoxins using molecularly imprinted polymers

Cited by 29 publications

References 162 publications

Study on Molecularly Imprinted Polymers Obtained Sonochemically for the Determination of Aflatoxins in Food

Study on Molecularly Imprinted Polymers Obtained Sonochemically for the Determination of Aflatoxins in Food

Nanomaterials for molecular recognition: specific adsorption and regulation of nanozyme activities

Microelectromechanical Microsystems-Supported Photothermal Immunoassay for Point-of-Care Testing of Aflatoxin B1 in Foodstuff

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