Programming Mechanical and Physicochemical Properties of 3D Hydrogel Cellular Microcultures via Direct Ink Writing

McCracken, Joselle M.; Badea, Adina; Kandel, Mikhail E.; Gladman, A. Sydney; Wetzel, David J.; Popescu, Gabriel; Lewis, Jennifer A.; Nuzzo, Ralph G.

doi:10.1002/adhm.201500888

Cited by 36 publications

(27 citation statements)

References 73 publications

(28 reference statements)

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“…3D bioprinting provides a novel approach to the design and fabrication of complex tissue constructs in vitro . It allows for the integration of numerous combinations of living cells and supporting matrices with precise spatial control, resulting in the engineering of numerous tissues with promising biomedical applications . The bioprinting technique has been demonstrated to offer new possibilities in advancing cancer research by creating vascularized tissues and positioning tumor cell‐laden hydrogels .…”

Section: Methodsmentioning

confidence: 99%

3D Bioprinted In Vitro Metastatic Models via Reconstruction of Tumor Microenvironments

et al. 2019

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The development of 3D in vitro models capable of recapitulating native tumor microenvironments could improve the translatability of potential anticancer drugs and treatments. Here, 3D bioprinting techniques are used to build tumor constructs via precise placement of living cells, functional biomaterials, and programmable release capsules. This enables the spatiotemporal control of signaling molecular gradients, thereby dynamically modulating cellular behaviors at a local level. Vascularized tumor models are created to mimic key steps of cancer dissemination (invasion, intravasation, and angiogenesis), based on guided migration of tumor cells and endothelial cells in the context of stromal cells and growth factors. The utility of the metastatic models for drug screening is demonstrated by evaluating the anticancer efficacy of immunotoxins. These 3D vascularized tumor tissues provide a proof‐of‐concept platform to i) fundamentally explore the molecular mechanisms of tumor progression and metastasis, and ii) preclinically identify therapeutic agents and screen anticancer drugs.

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Section: Methodsmentioning

confidence: 99%

3D Bioprinted In Vitro Metastatic Models via Reconstruction of Tumor Microenvironments

et al. 2019

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“…Recent innovations in 3D printing also offer flexibility in material selection, including cells, semiconductors, metals, ceramics, and polymers . Hence, 3D printing has resulted in the manufacturing of a variety of functional materials and devices, including soft sensors, electronics, biomedical devices, and artificial tissues and organs . In addition, recent developments in computer modeling, ink compositions, and extrusion‐based deposition techniques have enabled (i) multimaterial 3D printing techniques that allow for the fabrication of 3D objects with higher levels of complexity and functional performance, and (ii) the combination of 3D imaging technologies and 3D printing for next‐generation, patient‐specific biomanufacturing initiatives .…”

mentioning

confidence: 99%

3D Printed Stretchable Tactile Sensors

et al. 2017

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The development of methods for the 3D printing of multifunctional devices could impact areas ranging from wearable electronics and energy harvesting devices to smart prosthetics and human–machine interfaces. Recently, the development of stretchable electronic devices has accelerated, concomitant with advances in functional materials and fabrication processes. In particular, novel strategies have been developed to enable the intimate biointegration of wearable electronic devices with human skin in ways that bypass the mechanical and thermal restrictions of traditional microfabrication technologies. Here, a multimaterial, multiscale, and multifunctional 3D printing approach is employed to fabricate 3D tactile sensors under ambient conditions conformally onto freeform surfaces. The customized sensor is demonstrated with the capabilities of detecting and differentiating human movements, including pulse monitoring and finger motions. The custom 3D printing of functional materials and devices opens new routes for the biointegration of various sensors in wearable electronics systems, and toward advanced bionic skin applications.

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“…64,65 Many ECM proteins contain the arginine-glycine-aspartic acid (RGD) amino acid sequence as their cell attachment site. 66 The RGD sites of these adhesive proteins are recognized by a family of cell membrane receptors called integrins.…”

Section: Resultsmentioning

confidence: 99%

“…We have shown these physicochemical features to broadly impact the cell growth compliance of both pHH films and 3D microscaffolds in NIH/3T3 murine fibroblast and MC3T3-E1 cell cultures. 64 When modified with poly-D-lysine (PDL), these microperiodic scaffolds also allow the development and guidance of networks of rat primary hippocampal neurons. 65 …”

Section: Introductionmentioning

confidence: 99%

3D-Printed pHEMA Materials for Topographical and Biochemical Modulation of Dorsal Root Ganglion Cell Response

Badea

McCracken

Tillmaand

et al. 2017

ACS Appl. Mater. Interfaces

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Understanding and controlling the interactions occurring between cells and engineered materials are central challenges towards progress in the development of biomedical devices. In this work, we describe materials for direct ink writing (DIW), an extrusion-based type of 3D printing, that embed a custom synthetic protein (RGD-PDL) within the microfilaments of 3D-hydrogel scaffolds to modify these interactions and differentially direct tissue-level organization of complex cell populations in vitro. The RGD-PDL is synthesized by modifying poly-D-lysine (PDL) to varying extents with peptides containing the integrin-binding motif Arg-Gly-Asp (RGD). Compositional gradients of the RGD-PDL presented by both patterned and thin-film poly-(2-hydroxyethyl) methacrylate (pHEMA) substrates allow the patterning of cell-growth compliance in a grayscale form. The surface chemistry-dependent guidance of cell growth on the RGD-PDL-modified pHEMA materials is demonstrated using a model NIH-3T3 fibroblast cell line. The formation of a more complex cellular system — organotypic primary murine dorsal root ganglion (DRG) – in culture is also achieved on these scaffolds, where distinctive forms of cell growth and migration guidance are seen depending on their RGD-PDL content and topography. This experimental platform for the study of physicochemical factors on the formation and the reorganization of organotypic cultures offers useful capabilities for studies in tissue engineering, regenerative medicine, and diagnostics.

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Programming Mechanical and Physicochemical Properties of 3D Hydrogel Cellular Microcultures via Direct Ink Writing

Cited by 36 publications

References 73 publications

3D Bioprinted In Vitro Metastatic Models via Reconstruction of Tumor Microenvironments

3D Bioprinted In Vitro Metastatic Models via Reconstruction of Tumor Microenvironments

3D Printed Stretchable Tactile Sensors

3D-Printed pHEMA Materials for Topographical and Biochemical Modulation of Dorsal Root Ganglion Cell Response

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