Collaboration among nonprofits, commercial entities, state Extension offices, and governmental agencies is crucial for improving and sustaining food and nutrition security at the state and local levels. This study examines the landscape of 904 food and agricultural nonprofit organizations (FANOs) operating in Florida in 2019 to determine their scope, role in the food supply chain, and potential collaboration opportunities with allied organizations. FANOs primarily address the preproduction and distribution stages of the supply chain. Aligned goals and initiatives between FANOs and Extension programs revealed a wealth of opportunities for collaboration that would ensure the continuity of food systems and sustainable resource use in Florida, despite existing competition between allied organizations and FANOs.