Programmed Materials Synthesis with DNA

Storhoff, James J.; Mirkin, Chad A.

doi:10.1021/cr970071p

Cited by 1,022 publications

(733 citation statements)

References 90 publications

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“…Significant advances in biosensor development, 1-4 materials fabrication, [5][6][7][8][9] nanomedicine, 10 and nanotechnology 11 have already been demonstrated. To conjugate a DNA to AuNPs, the following two strategies have been employed.…”

Section: This Document Is the Accepted Manuscript Version Of A Publismentioning

confidence: 99%

Instantaneous Attachment of an Ultrahigh Density of Nonthiolated DNA to Gold Nanoparticles and Its Applications

et al. 2012

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The last 16 years have witnessed the landmark development of polyvalent thiolated DNA functionalized gold nanoparticles (AuNPs) possessing striking properties within the emerging field of nanobiotechnology. Many novel properties of this hybrid nanomaterial are attributed to the dense DNA shell. However, the question of whether non-thiolated polyvalent DNA-AuNP could be fabricated with high DNA density and similar properties as its thiolated counterpart has not been explored in detail.Herein, we report that by simply tuning the pH of the DNA/AuNP mixture, an ultrahigh capacity of nonthiolated DNA can be conjugated to AuNPs in a few minutes, resulting in polyvalent DNA-AuNP conjugates with cooperative melting behavior, a typical property for polyvalent thiolated DNA functionalized AuNPs. With this method, large AuNPs (e.g., 50 nm) can be functionalized to achieve colorimetric detection of sub-nM DNA. Further, this fast and stable DNA loading was employed to separate AuNPs of different size. We propose that a large fraction of the attached DNAs are adsorbed via one or a few terminal bases to afford the high loading capacity and the ability to hybridize with the complementary DNA. This discovery not only offers a time-and cost-effective way to functionalize AuNPs with a high density of non-thiolated DNA, but also provides new insights into the fundamental understanding of how DNA strands with different sequences interact with AuNPs.

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Section: This Document Is the Accepted Manuscript Version Of A Publismentioning

confidence: 99%

Instantaneous Attachment of an Ultrahigh Density of Nonthiolated DNA to Gold Nanoparticles and Its Applications

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Due to the modulate capability and diverse ligand linkage on GNP surface, GNPs can be used in many fields such as organic reactions, sensors (inorganic or biologic), biological diagnostic, drug and DNA delivery and imaging [11][12][13][14]. Although the linkage of sulphur-bearing groups to GNPs and also linking different ligands to them are very appealing, practical and useful [11][12][13][14][15], the amine groups binding to GNPs can be considered in the same level.…”

Section: Introductionmentioning

confidence: 99%

“…Although the linkage of sulphur-bearing groups to GNPs and also linking different ligands to them are very appealing, practical and useful [11][12][13][14][15], the amine groups binding to GNPs can be considered in the same level. In fact, the linkage of GNPs to alkyl amine can facilitate the transfer of GNPs to organic solvents [16], and also the strong linkage of amino groups in amino acids and proteins to GNPs surface are related to the medical application of these hybrid materials [17,18].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of capped gold nanoparticles by using various amino acids

Zare

Khoshnevisan

Barkhi

et al. 2013

Journal of Experimental Nanoscience

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The production of gold nanoparticles (GNPs) by amino acid is one of the most attractive and interesting subjects in nanobiotechnology. In this study, amino acids have been utilised as a reducing agent and also an agent for capping GNPs. The GNPs were prepared using a reduction solution containing gold cations with optimum concentration of gold salt (5 mM), and also functionalised by glutamic acid, phenylalanine and tryptophan with optimum concentration of amino acids (25 mM). The optimum condition of gold solution and amino acids were achieved by ultraviolet-visible spectroscopy. The size of nanoparticles was obtained 5-20, 10-20 and 20-30 nm, respectively, by transmission electron microscopy and dynamic light scattering techniques. The results obtained from experimental and quantum calculations confirm that amino acids have strong bond while they have anion binding. Moreover, the free carboxylic groups of capped GNPs are one of the suitable and capable beads for binding biological agents. As a result, the medical applications of amino acids and proteins can be used as a practical method due to the strong interaction of peripheral amine groups with nanoparticles.

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“…14,15 DNA carries the functional roles of molecular recognition and can also act as an antisense agent. 1,16 Gold nanoparticles (AuNPs) and graphene oxide (GO) are two representative nanomaterials for interacting with DNA.…”

Section: Introductionmentioning

confidence: 99%

Effects of Polyethylene Glycol on DNA Adsorption and Hybridization on Gold Nanoparticles and Graphene Oxide

et al. 2012

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Understanding the interface between DNA and nanomaterials is crucial for rational design and optimization of biosensors and drug delivery systems. For detection and delivery into cells, where high concentrations of cellular proteins are present, another layer of complexity is added. In this context, we employ polyethylene glycol (PEG) as a model polymer to mimic the excluded volume effect of cellular proteins and test its effect on DNA adsorption and hybridization on gold nanoparticles (AuNPs) and graphene oxide (GO), both of which show great promise for designing intracellular biosensors and drug delivery systems. We show that PEG 20,000 (e.g. 4%) accelerates DNA hybridization to DNAfunctionalized AuNPs by 50-100%, but this enhanced hybridization kinetics has not been observed with free DNA. Therefore, this rate enhancement is attributed to the surface blocking effect by PEG instead of the macromolecular crowding effect. On the other hand, DNA adsorption on citrate-capped AuNP surfaces is impeded even in the presence of a trace level (i.e., parts-per-billion) of PEG, confirming PEG competes with DNA for surface binding sites. Additional insights have been obtained by studying the adsorption of a thiolated DNA and a peptide nucleic acid. In these cases, the steric effects of PEG to impede adsorption are observed. Similar observations have also been made with GO. Therefore, PEG may be used as an effective blocking agent for both hydrophilic AuNP and for GO that also contains hydrophobic domains.

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Programmed Materials Synthesis with DNA

Cited by 1,022 publications

References 90 publications

Instantaneous Attachment of an Ultrahigh Density of Nonthiolated DNA to Gold Nanoparticles and Its Applications

Instantaneous Attachment of an Ultrahigh Density of Nonthiolated DNA to Gold Nanoparticles and Its Applications

Fabrication of capped gold nanoparticles by using various amino acids

Effects of Polyethylene Glycol on DNA Adsorption and Hybridization on Gold Nanoparticles and Graphene Oxide

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