Programmed cell death in wheat (Triticum aestivum L.) endosperm cells is affected by drought stress

Li, Chao; Cheng, Li; Wang, Bingbing; Zhang, Runqi; Fu, Kaiyong; Gale, William F.; Li, Chunyan

doi:10.1007/s00709-018-1203-7

Cited by 36 publications

(28 citation statements)

References 58 publications

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“…Fertigrain Foliar, a leaf biostimulant that facilitates the vegetative development of plants; promotes better grain ripening; increases ear-grain content; builds stress resistance; improves productivity and yielding capacity; eliminates micronutrient deficiencies. The composition is 5% nitrogen, 40% organic matter, 0.75% zinc, 0.5% manganese, 0.1% copper, 0.1% boron, 0.1% iron, 0.02% molybdenum, 0.01 % cobalt, 8% free L amino acids (dose in the experiment -1 l/t) (3,(17)(18)(19).…”

Section: Methodsmentioning

confidence: 99%

The Structure and Productivity of Winter Durum Wheat Subject to Pre-Sowing Treatment

Podlesnykh¹,

Galochkina²

2018

International Scientific and Practical Conference "AgroSMART - Smart Solutions for Agriculture" (AgroSMART 2018)

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The paper studies the productivity of winter durum wheat of Zolotko variety subject to the treatment of seed grains with the preparations Poly-Feed, Plodorodiye Sibiri, Vitazim, Agat-25K, Micromak, potassium humate applied separately and in the mixture of Poly-Feed + Plodorodiye Sibiri + Vitazim followed by the treatment of plants with Vitazim, Fertigrain Foliar and the mixture of Vitazim + Fertigrain Foliar in the forest-steppe of the Central Chernozem Region. The experiments were conducted in 2014/15-2015/16 in the fields of Voronezh SAU. For the treatment of winter durum wheat seeds, the most effective preparations to increase tillering capacity were Agat-25K (1.18 seeds/plant), Vitazim (1.17 seeds/plant) and potassium humate (1.17 seeds/plant). The number of kernels per head increased provided that the seeds were treated with Agat-25K, Micromak, Plodorodiye Sibiri, especially followed by the further treatment of plants with the mixture of Vitazim + Fertigrain Foliar. The weight of grains in the ear increased up to 1.28 and 1.27g when the seeds were treated with Agat-25K and Micromak jointly with the treatment of plants with Vitazim + Fertigran Foliar. A thousand grain weight was greater when the seeds were treated with Agat 25K, Micromak and potassium humate jointly with the treatment of plants with a mixture of Vitazim + Fertigrean Foliar and amounted to 45.0; 44.9 and 44.85g, respectively. A high yield was obtained when Agat-25K and Micromak were used for treating seeds, together with treating plants with a mixture of Vitazim + Fertigrean Foliar. It amounted to 52.7 and 52.2 c/ha, respectively. The highest synergetic efficiency was observed when treating the seeds with Agat-25K or Micromak together with treating the plants with Vitazim + Fertigrain Foliar mixture.

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Section: Methodsmentioning

confidence: 99%

The Structure and Productivity of Winter Durum Wheat Subject to Pre-Sowing Treatment

Podlesnykh¹,

Galochkina²

2018

International Scientific and Practical Conference "AgroSMART - Smart Solutions for Agriculture" (AgroSMART 2018)

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“…The dead cells can be stained blue by Evans Blue, a dye that is excluded from living cells with intact plasma membrane and stains only the cytoplasm of nonviable cells [27]. For example, the pattern and progression of programmed cell death in cereal endosperm are detected by staining fresh freehand sections of developing kernels with Evans Blue [10,[25][26][27][28] (Fig. 1d).…”

Section: Free-hand Sectionmentioning

confidence: 99%

“…Another important application of paraffin sections is the histochemistry and immunohistochemistry of cereal kernel. For example, the shape and size of nuclei in endosperm cells can be observed using paraffin section stained with 4′,6-diamidino-2-phenylindole under a fluorescence microscope [28,39] (Fig. 4b).…”

Section: Paraffin Sectionmentioning

confidence: 99%

Comprehensive comparison and applications of different sections in investigating the microstructure and histochemistry of cereal kernels

Wei

2020

Plant Methods

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This review summarizes the main applications of different sections and some improved sectioning methods in investigating the microstructure and histochemistry of cereal kernels. Thick sections of developing kernels prepared by free-hand and sliding microtome-aided sectioning method can be employed to elucidate tissue anatomy and histochemistry. The thin sections of mature kernels prepared by ultramicrotome-aided sectioning method can exhibit the micromorphology of starch granules when stained with iodine solution. The paraffin sections of developing kernels can exhibit the tissue anatomy of kernel, the accumulation of storage substances, and the location of protein and gene transcripts with immunohistochemistry and in situ hybridization techniques. The semithin resin sections can clearly exhibit the morphology of cells, starch granules, and protein bodies in kernel, but the sections prepared with different resins have various advantages and disadvantages for research investigating the morphology and histochemistry of cereal kernels. The improved methods of free-hand sectioning and ultramicrotome-aided sectioning of mature kernels are suitable for investigating the morphology of starch granules in a large number of samples in a short time. The modified method for preparing resin sections of whole kernels can be employed to determine the morphology and distribution of cells, starch granules, and storage protein in mature, developing, germinated, and cooked kernels in situ. This review could help researchers choose appropriate sections for investigating the microstructure and histochemistry of cereal kernels according to their study objectives.

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“…This implied that the process of PCD in endosperm cells of weedy rice was faster than that of cultivated rice, and this may be one of the important physiological mechanisms of early maturity in weedy rice. Plant endogenous hormones are closely related to the process of PCD, altering endogenous hormone concentrations during grain lling could delay endosperm PCD, increasing grain lling time, such as abscisic acid (ABA), ethylene, and gibberellic acid (GA) can regulate PCD in the developing endosperm Gallie 1999, 2000;Li et al 2018). However, the difference of hormone content between weedy rice and cultivated rice and its relationship with PCD during grain lling need to be further studied.…”

Section: Comparison Of Anti-oxidative Enzymes System Between Weedy Rimentioning

confidence: 99%

“…In the process of PCD of rice endosperm, the nucleus is the rst to die out, and then the cells still maintain high physiological activity Gallie 1997, 1999). In wheat endosperm, PCD occurred randomly, while in rice, PCD rst occurred in the middle of grain, and then gradually developed to the edge Lan et al 2004;Li et al 2018). Endosperm is the main material of rice seeds, accounting for 91% − 92% of the total weight of rice seeds.…”

Section: Introductionmentioning

confidence: 99%

Rapid PCD Process Promotes Early Maturity in Weedy Rice (Oryza sativa L. f. spontanea)

Zhao

Zhang

et al. 2020

Preprint

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Background: Shorter grain-filling period and rapid endosperm development contributes to early maturity in weedy rice (Oryza sativa L. f. spontanea). However, the differences in programmed cell death (PCD) process and anti-oxidative enzymes system in the caryopsis between weedy and cultivated rice are largely unexplored. Main Text: we selected four biotypes of weedy rice and associated cultivated rice (ACR, Oryza sativa) from different latitudes to conduct a common garden experiment. The difference of PCD process between weedy rice and ACR was compared by chemical staining, and the cell viability and nuclear morphometry of endosperm cells were observed by optical microscopy, and anti-oxidative enzymes activity were also measured during grain filling. We found that the PCD progress in weedy rice was more rapid and earlier than that in ACR. The percentage of degraded nuclei of weedy rice were 10%-83% higher than that of ACR. Endosperm cells in weedy rice lost cell viability 2-8 days earlier than that in ACR. The anti-oxidant enzymes activity of weedy rice were lower than that of ACR during grain filling. The ability of weedy rice to scavenge reactive oxygen species is weaker than that of ACR, which may contribute to the rapid PCD process in the endosperm cells of weedy rice. Conclusion: The rapid PCD process and weaker ability to scavenge reactive oxygen species in endosperm cells lead to the shorter grain-filling period of weedy rice.

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Programmed cell death in wheat (Triticum aestivum L.) endosperm cells is affected by drought stress

Cited by 36 publications

References 58 publications

The Structure and Productivity of Winter Durum Wheat Subject to Pre-Sowing Treatment

The Structure and Productivity of Winter Durum Wheat Subject to Pre-Sowing Treatment

Comprehensive comparison and applications of different sections in investigating the microstructure and histochemistry of cereal kernels

Rapid PCD Process Promotes Early Maturity in Weedy Rice (Oryza sativa L. f. spontanea)

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