Programmed cell death in Acanthamoeba castellanii Neff induced by several molecules present in olive leaf extracts

Abstract

Therapy against Acanthamoeba infections such as Granulomatous Amoebic Encephalitis (GAE) and Acanthamoeba Keratitis (AK), remains as an issue to be solved due to the existence of a cyst stage which is highly resistant to most chemical and physical agents. Recently, the activity of Olive Leaf Extracts (OLE) was demonstrated against Acanthamoeba species. However, the molecules involved in this activity were not identified and/or evaluated. Therefore, the aim of this study was to evaluate the activity of the main molecules which are present in OLE and secondly to study their mechanism of action in Acanthamoeba. Among the tested molecules, the observed activities ranged from an IC50 of 6.59 in the case of apigenine to an IC50 > 100 μg/ml for other molecules. After that, elucidation of the mechanism of action of these molecules was evaluated by the detection of changes in the phosphatidylserine (PS) exposure, the permeability of the plasma membrane, the mitochondrial membrane potential and the ATP levels in the treated cells. Vanillic, syringic and ursolic acids induced the higher permeabilization of the plasma membrane. Nevertheless, the mitochondrial membrane was altered by all tested molecules which were also able to decrease the ATP levels to less than 50% in IC90 treated cells after 24 h. Therefore, all the molecules tested in this study could be considered as a future therapeutic alternative against Acanthamoeba spp. Further studies are needed in order to establish the true potential of these molecules against these emerging opportunistic pathogenic protozoa.

G418 induces programmed cell death in Acanthamoeba through the elevation of intracellular calcium and cytochrome c translocation

“…In agreement with former studies (Martín-Navarro et al 2015 ; Martín-Navarro et al 2017 ; Sifaoui et al 2017 ; Baig et al 2017 ; Lopez-Arencibia et al 2017 ; Moon et al 2018 ), we have found morphological changes in Acanthamoeba associated with a PCD-linked series of events. These include rounded up, cell shrinkage, intracellular ion fluctuations, mitochondrial dysfunction nuclear and chromatin condensation and finally breakup and release of apoptotic body-like particles.…”

Toxic effects of selected proprietary dry eye drops on Acanthamoeba

“…The early stage was inferred from phosphatidylserine externalization and chromatin condensation. Since this initial report we 20 , 21 and others 22 , 23 have reported a number of PCD inducing agents in Acanthamoeba . In these reports, authors have been able to distinguish between early PCD cells, late PCD cells.…”

Phytochemical screening and antioxidant activity of some medicinal plants’ crude juices

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