Programmed cell death during androgenesis in Hordeum vulgare L.

Wang, M.; Bergen, S. van; Lamers, G.E.M.; Oppedijk, B.; Schilperoort, R. A.

doi:10.1007/978-3-642-59985-9_19

Cited by 7 publications

(3 citation statements)

References 24 publications

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“…In winter cultivar Igri, it was recently shown that DNA is degraded in the anther during pollen development, indicating apoptosis in this organ (Wang et al 1999a). Barley is a species with maternal cytoplasmic inheritance and microspore plastids are particularly affected by DNA degradation during pollen development (Mogensen 1996).…”

Section: Discussionmentioning

confidence: 99%

“…Most studies aimed at improving the androgenesis protocol or elucidating the androgenic mechanisms in barley are performed using the winter cultivar Igri as a control Wang et al 1999a). In this cultivar optimizing the different steps of androgenesis makes it possible to greatly reduce the proportion of albino regenerated plantlets under both anther and microspore culture (Ziauddin et al 1990;Jähne et al 1991;Hoekstra et al 1993;).…”

Section: Introductionmentioning

confidence: 99%

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Plastid differentiation during androgenesis in albino and non-albino producing cultivars of barley ( Hordeum vulgare L.)

Caredda

Doncoeur

Devaux³

et al. 2000

Sexual Plant Reproduction

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In order to better understand androgenic albinism in barley, we compared plastid differentiation during anther culture in two cultivars, an albino (spring cultivar Cork) and a non-albino (winter cultivar Igri) producing cultivar. The ultrastructure of plastids and the relative amount of DNA containing plastids were followed in both cultivars during the androgenic process and correlated with the proportion of regenerated chlorophyllous plantlets.For androgenesis, anthers were collected at the uninucleate stage, during mid-or late-microspore vacuolation. At this stage DNA was detected in 15.3 ± 2. 7% of microspore plastid sections in the winter cultivar Igri, compared to 1.7 ± 0.5% in the spring cultivar Cork. In the winter cultivar Igri, starch was broken down after anther pretreatment but plastids divided rapidly during anther culture and thylakoids developed in the stroma. Prior to regeneration, plastids contained 2.0 ± 0.2 thylakoids per plastid and starch represented 26.1 ± 3.3% of the plastid volume. In the spring cultivar Cork, plastids followed a different developmental pathway. After anther pretreatment, microspore plastids differentiated exclusively into amyloplasts, accumulating starch and losing their thylakoids as well as their capacity to divide. This developmental pattern became progressively more marked, so that by the end of anther culture plastids contained 0.5 ± 0.4 thylakoids per plastid and starch represented up to 90.3 ± 4.3% of plastid volume. Following androgenesis, the response was similar in both cultivars except that the winter cultivar Igri pro-vided 87.8% of chlorophyllous plantlets compared to 99.7% albino plantlets in the cultivar Cork.The results presented here suggest that the exclusive regeneration of albino plantlets in the spring cultivar Cork may be due to degradation of microspore plastid DNA during early pollen development, preventing the plastids from differentiating into chloroplasts under culture conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Plastid differentiation during androgenesis in albino and non-albino producing cultivars of barley ( Hordeum vulgare L.)

Caredda

Doncoeur

Devaux³

et al. 2000

Sexual Plant Reproduction

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“…Changes in the permeability of the outer mitochondrial membrane and the release of cytochrome c (CYT c) and apoptotic proteins in mitochondria promote PCD in animal cells, and after the release of CYT c, cells die due to disruption of electron transport, resulting in the rapid production of reactive oxygen species and a decrease in the levels of adenosine triphosphate (Hoeberichts and Woltering 2003). During apoptosis in plants, cytochromes released by mitochondria can initiate PCD in myeloid cells (Wang 2021). Our study confirmed changes in the structure of the mitochondrial membrane during apoptosis, although the function of mitochondria in the aging of peony petals and PCD still needs to be studied.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Programmed Cell Death in Aging <i>Paeonia lactiflora</i> Petals

Sun

Silva

et al. 2023

CYTOLOGIA

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In order to study the ultrastructural changes and characteristics associated with programmed cell death (PCD) in the petals of Paeonia lactiflora during senescence, scanning electron microscopy and transmission electron microscopy were used to observe the surface and interior of perianth slices of P. lactiflora ʻZhong Sheng Fenʼ flower petals at different blooming stages. Observations indicated that PCD in parenchyma cells occurred at an early flowering stage, even though visible petal senescence was manifested during the collapse of epidermal cells at the withering stage. The epidermal cells of the perianth were fully extended at the blooming stage and had cristae on the cell surface that were continuously distributed over the long axis of cells, while different degrees of contraction of the intercellular space was observed. The progression of PCD displayed several characteristics: vacuolar membrane rupture and organelle degradation, nuclear membrane rupture and nonuniform cell wall degradation, and cell membrane rupture and changes to the cell wall, while vacuole degradation was likely a key event marking the start of PCD. TUNEL results further confirmed the occurrence of PCD during petal senescence.

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Microspore Embryo Induction and Development in Higher Plants: Cytological and Ultrastructural Aspects

Clément

Sangwan

Sangwan‐Norreel

Haploids in Crop Improvement II

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Programmed cell death during androgenesis in Hordeum vulgare L.

Cited by 7 publications

References 24 publications

Plastid differentiation during androgenesis in albino and non-albino producing cultivars of barley ( Hordeum vulgare L.)

Plastid differentiation during androgenesis in albino and non-albino producing cultivars of barley ( Hordeum vulgare L.)

Assessment of Programmed Cell Death in Aging <i>Paeonia lactiflora</i> Petals

Microspore Embryo Induction and Development in Higher Plants: Cytological and Ultrastructural Aspects

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