Programmatic implementation of blastocyst transfer in a university-based in vitro fertilization clinic: maximizing pregnancy rates and minimizing triplet rates

In vitro fertilization with preimplantation genetic screening improves implantation and live birth in women age 40 through 43

“…ICSI was performed for patients diagnosed with male factor infertility or a history of failed fertilization. Normally fertilized zygotes exhibiting two pronuclei (PN) were cultured to day 3 in 30 ul droplets of culture media (Quinn's Cleavage Medium, Cooper Surgical) and monitored for embryonic development as previously described [24]. Both the fresh IVF and PGS group had same criteria for culture to day 5/6.…”

Comparison of pregnancy outcomes in elective single blastocyst transfer versus double blastocyst transfer stratified by age

“…The decision to culture to day 5 was based on the quantity and quality of embryos on day 3. In short, if an excess number of good quality embryos are available for transfer (generally R3 embryos), then embryo culture would be continued (15). Embryos were graded on day 5 using the Gardner and Lane criteria (16) and assigned a numerical score (1-6) according to the stage of development, from early to hatched blastocyst and a letter designation (a, b, c, or d) separately for both the inner cell mass (ICM) and the trophectoderm.…”

Comparison of pregnancy outcomes in anonymous shared versus exclusive donor oocyte in vitro fertilization cycles

“…The decision to culture to day 5 was based on the quantity and quality of embryos on day 3. If R 3 good quality embryos were available for transfer, then embryo culture would be continued to day 5 (11). Embryos were graded on day 5 using the Gardner and Lane criteria (12) and assigned a numerical score (1)(2)(3)(4)(5)(6) according to the stage of development, from early to hatched blastocyst and a letter designation (a, b, c, or d) separately for both the inner cell mass and the trophectoderm.…”