Programmable wireless light-emitting diode stimulator for chronic stimulation of optogenetic molecules in freely moving mice

Hashimoto, Mitsuhiro; Hata, Akinori; Miyata, Takaki; Hirase, Hajime

doi:10.1117/1.nph.1.1.011002

Cited by 62 publications

(39 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The optical intensity generated in the nontargeted channel as a function of that in the targeted channel appears in Fig. 2 E and F. As indicated by the dotted lines, the intensity can reach ∼12 mW/mm 2 , which is more than sufficient to activate optogenetic proteins, before the onset of the operation in the nontargeted channel (21)(22)(23). The direct proximity of the light emitters in these injectable optoelectronic systems to the regions of interest in the brain enables activation of light-sensitive proteins with intensities of ∼10 mW/mm 2 , much lower than those needed at the light source regions of systems that require delivery of light via use of waveguides (∼100 mW/mm 2 ) (24).…”

Section: Resultsmentioning

confidence: 97%

Stretchable multichannel antennas in soft wireless optoelectronic implants for optogenetics

Park

Shin

McCall

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

114

101

View full text Add to dashboard Cite

Optogenetic methods to modulate cells and signaling pathways via targeted expression and activation of light-sensitive proteins have greatly accelerated the process of mapping complex neural circuits and defining their roles in physiological and pathological contexts. Recently demonstrated technologies based on injectable, microscale inorganic light-emitting diodes (μ-ILEDs) with wireless control and power delivery strategies offer important functionality in such experiments, by eliminating the external tethers associated with traditional fiber optic approaches. Existing wireless μ-ILED embodiments allow, however, illumination only at a single targeted region of the brain with a single optical wavelength and over spatial ranges of operation that are constrained by the radio frequency power transmission hardware. Here we report stretchable, multiresonance antennas and battery-free schemes for multichannel wireless operation of independently addressable, multicolor μ-ILEDs with fully implantable, miniaturized platforms. This advance, as demonstrated through in vitro and in vivo studies using thin, mechanically soft systems that separately control as many as three different μ-ILEDs, relies on specially designed stretchable antennas in which parallel capacitive coupling circuits yield several independent, well-separated operating frequencies, as verified through experimental and modeling results. When used in combination with active motion-tracking antenna arrays, these devices enable multichannel optogenetic research on complex behavioral responses in groups of animals over large areas at low levels of radio frequency power (<1 W). Studies of the regions of the brain that are involved in sleep arousal (locus coeruleus) and preference/aversion (nucleus accumbens) demonstrate the unique capabilities of these technologies.wireless optogenetics | stretchable electronics | wireless power transmission | deep brain stimulation | antenna O ptogenetics exploits a toolbox of light-sensitive proteins for optical manipulation of neural networks as a powerful means for the study of circuit-level mechanisms that underlie psychiatric diseases (1-4). Canonical optogenetic experiments in the brain require cranial insertion of an optical fiber to illuminate a region of interest (5, 6). Although this approach permits simple behavior modeling, constraints in animal motion and alterations in natural behaviors due to fiber tethering and external fixation complicate use in chronic longitudinal models and in experiments that assess complex responses. Many of these limitations can be bypassed with optoelectronic technologies and wireless receivers, as recently demonstrated in optogenetic stimulation of the brain, the peripheral nerves, and the spinal cord (4, 7-13). Systems that offer soft, compliant mechanical properties and thin, fully implantable designs are particularly advantageous (7). These systems, however, have still not been optimized to fully take advantage of the power of combining mouse genetics/optogentics with long-term beha...

show abstract

Section: Resultsmentioning

confidence: 97%

Stretchable multichannel antennas in soft wireless optoelectronic implants for optogenetics

Park

Shin

McCall

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

114

101

View full text Add to dashboard Cite

show abstract

“…Researchers have developed both wirelessly powered 8,9 and battery-powered 10,11 devices that deliver light to the surface of the mouse brain with an LED. Deeper brain regions can also be targeted with a flexible, injectable LED system and the option to wirelessly power through a head-mountable receiver 12,13 or with a battery-powered, modular device using commercially available components 14 .…”

mentioning

confidence: 99%

Wirelessly powered, fully internal optogenetics for brain, spinal and peripheral circuits in mice

et al. 2015

View full text Add to dashboard Cite

To enable sophisticated optogenetic manipulation of neural circuits throughout the nervous system with limited disruption of animal behavior, light-delivery systems beyond fiber optic tethering and large, head-mounted wireless receivers are desirable. We report the development of an easy-to-construct, implantable wireless optogenetic device. Our smallest version (20 mg, 10 mm3) is two orders of magnitude smaller than previously reported wireless optogenetic systems, allowing the entire device to be implanted subcutaneously. With a radio-frequency (RF) power source and controller, this implant produces sufficient light power for optogenetic stimulation with minimal tissue heating (<1 °C). We show how three adaptations of the implant allow for untethered optogenetic control throughout the nervous system (brain, spinal cord and peripheral nerve endings) of behaving mice. This technology opens the door for optogenetic experiments in which animals are able to behave naturally with optogenetic manipulation of both central and peripheral targets.

show abstract

“…Wireless systems for neural modulation have been developed in order to eliminate tethers [5][6][7][8][9][10][11][12]. Most systems for mice need to be wirelessly powered due to the substantial size and weight of batteries relative to that of the animal [6,13].…”

mentioning

confidence: 99%

Self-Tracking Energy Transfer for Neural Stimulation in Untethered Mice

Tanabe

Iyer

et al. 2015

Phys. Rev. Applied

View full text Add to dashboard Cite

Optical or electrical stimulation of neural circuits in mice during natural behavior is an important paradigm for studying brain function. Conventional systems for optogenetics and electrical microstimulation require tethers or large head-mounted devices that disrupt animal behavior. We report a method for wireless powering of small-scale implanted devices based on the strong localization of energy that occurs during resonant interaction between a radio-frequency cavity and intrinsic modes in mice. The system features self-tracking over a wide (16 cm diameter) operational area, and is used to demonstrate wireless activation of cortical neurons with miniaturized stimulators (10 mm 3 , 20 mg) fully implanted under the skin.

show abstract

Programmable wireless light-emitting diode stimulator for chronic stimulation of optogenetic molecules in freely moving mice

Cited by 62 publications

References 16 publications

Stretchable multichannel antennas in soft wireless optoelectronic implants for optogenetics

Stretchable multichannel antennas in soft wireless optoelectronic implants for optogenetics

Wirelessly powered, fully internal optogenetics for brain, spinal and peripheral circuits in mice

Self-Tracking Energy Transfer for Neural Stimulation in Untethered Mice

Contact Info

Product

Resources

About