Programmable readout sensor for microRNA: CRISPR/Cas12a-assisted multi-amplification strategy activated photoelectrochemistry-colorimetry detection

Shen, Haoran; Yang, Hui Ying; Qileng, Aori; Ma, Yidan; Liang, Hongzhi; Meng, Jingnan; Lei, Hongtao; Liu, Yingju; Liu, Weipeng

doi:10.1016/j.snb.2022.132585

Cited by 14 publications

(11 citation statements)

References 31 publications

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“…In addition, different RCA reactions of the system can recognise different types of miRNAs. [105] On the detection of non-nucleic acids Wu et al prepared a colourimetric induced sensor for the detection of aflatoxin M 1 (AFM 1) using the catalytic activity of RCA and AuNPs. No long ssDNA was produced on the AuNP surface, and the addition of 4-nitrophenol converted it to 4- aminophenol (colour changed from yellow to colourless).…”

Section: On the Detection Of Nucleic Acidsmentioning

confidence: 99%

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Application of CRISPR/Cas Technology in Biological Nano‐Analysis

Liu,

Aodeng,

et al. 2023

ChemistrySelect

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Currently, with the growing global demand for infectious disease diagnostics, environmental testing and food safety, CRISPR‐Cas (Clustered Regularly Interspaced Short Palindromic Repeats‐CRISPR associated proteins) system has unlimited potential as one of the emerging technologies in the field of biotechnology. Recent studies have shown that CRISPR‐Cas system has become a powerful weapon for gene editing, disease treatment, molecular diagnosis and imaging. As important biomarkers, various nucleic acid and non‐nucleic acid targets need to be detected from various samples before the situation deteriorates. In addition, there are still some limitations and challenges in the current biosensor platforms based on the CRISPR‐Cas system. Therefore, summarising the strategies, strengths and weaknesses, and development prospects of the CRISPR‐Cas system in nucleic acid and non‐nucleic acid target identification is beneficial to fully explore the potential development directions of CRISPR‐Cas‐based biosensor systems. This review briefly introduces and summarises the classification, mechanisms and applications of the system (diagnostics when combined with some signal detection means). In addition, some of the current limitations, challenges and future prospects for optimisation of biosensor platforms based on this system are discussed.

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Section: On the Detection Of Nucleic Acidsmentioning

confidence: 99%

“…Finally, the results were detected by photoelectrochemical and colourimetric signals (Figure 15B). In addition, different RCA reactions of the system can recognise different types of miRNAs [105] …”

Section: Introductionmentioning

confidence: 99%

Application of CRISPR/Cas Technology in Biological Nano‐Analysis

Liu,

Aodeng,

et al. 2023

ChemistrySelect

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“…Thus, the highly sensitive detection of miRNA is essential to the investigation of the growth and progression of cancers. There are currently several methods to monitor miRNAs, − such as electrochemical luminescence, photochemistry, colorimetry, and fluorescence . For instance, a fluorescent method was designed for miRNA-21 detection based on Janus wireframe DNA cube and catalytic hairpin assembly .…”

Section: Introductionmentioning

confidence: 99%

Self-Powered Biosensor Based on DNA Walkers for Ultrasensitive MicroRNA Detection

Yang,

Wang,

et al. 2023

Anal. Chem.

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A novel self-powered biosensor is fabricated for ultrasensitive microRNA-21 (miRNA-21) detection, which includes an enzymatic biofuel cell (EBFC), DNA walkers, a digital multimeter (DMM), and a capacitor. As a novel strategy for signal amplification, DNA walkers are designed in the cathode, while the capacitor stores electrochemical energy from the EBFC to further boost the instantaneous current displayed by the DMM. When miRNA-21 is present, the DNA walkers are provoked to walk from as-opened hairpin structures to other hairpin structures, generating double-strand DNA structures, which stimulate [Ru(NH 3 ) 6 ] 3+ to be adsorbed on the cathode surface by electrostatic interaction. Afterward, [Ru(NH 3 ) 6 ] 3+ is reduced to [Ru-(NH 3 ) 6 ] 2+ , and the open circuit voltage (E OCV ) is significantly increased. Depending on the approach of signal amplification from DNA walkers, this biosensor displays an ultrasensitive assay toward miRNA-21 in the range of 0.5 to 10 4 fM, with a detection limit of 0.15 fM. In addition, this self-powered biosensor displays high selectivity for miRNA-21 assay in human serum samples.

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“…One is to use the trans-cleavage property of CRISPR/Cas12 system to cleave the initiation chain of the amplification reaction, such as the hybridization chain reaction (HCR) or rolling circle amplification (RCA), and inhibit the reaction process. 34,35 Another commonly used strategy is to transform the tested substance to produce more activation chains (crRNA or target of CRISPR/Cas12 system) to improve the sensitivity of the CRISPR/Cas12 system through a nucleic acid amplification strategy. 36−38 However, most of these strategies are based on enzyme-assisted amplification, which takes a segment of DNA as a template to form the new activation chain by deoxyribonucleoside triphosphate (dNTPs) with the help of polymerase.…”

mentioning

confidence: 99%

“…In recent years, the nucleic acid amplification techniques have been integrated with CRISPR/Cas12 to construct ECL sensors. − Currently, the reported amplification strategies based on the CRISPR/Cas12 system are mainly divided into two types. One is to use the trans-cleavage property of CRISPR/Cas12 system to cleave the initiation chain of the amplification reaction, such as the hybridization chain reaction (HCR) or rolling circle amplification (RCA), and inhibit the reaction process. , Another commonly used strategy is to transform the tested substance to produce more activation chains (crRNA or target of CRISPR/Cas12 system) to improve the sensitivity of the CRISPR/Cas12 system through a nucleic acid amplification strategy. − However, most of these strategies are based on enzyme-assisted amplification, which takes a segment of DNA as a template to form the new activation chain by deoxyribonucleoside triphosphate (dNTPs) with the help of polymerase. , This is because if the activation strand exists in the original nucleic acid, the original nucleic acid may activate Cas12a to produce leakage. In addition, the non-specific trans-cleavage property of Cas12a also splices original single-stranded nucleic acid.…”

mentioning

confidence: 99%

Sensitive and Amplification-Free Electrochemiluminescence Biosensor for HPV-16 Detection Based on CRISPR/Cas12a and DNA Tetrahedron Nanostructures

Yu,

Peng,

Sheng

et al. 2023

ACS Sens.

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Rapid and accurate detection of biomarkers was very important for early screening and treatment of diseases. Herein, a sensitive and amplification-free electrochemiluminescence (ECL) biosensor based on CRISPR/Cas12a and DNA tetrahedron nanostructures (TDNs) was constructed. Briefly, 3D TDN was selfassembled on the Au nanoparticle-deposited glassy carbon electrode surface to construct the biosensing interface. The presence of the target would activate the trans-cleavage activity of Cas12a-crRNA duplex to cleave the single-stranded DNA signal probe on the vertex of TDN, causing the Ru(bpy) 3 2+ to fall from the electrode surface and weakened the ECL signal. Thus, the CRISPR/Cas12a system transduced the change of target concentration into an ECL signal enabling the detection of HPV-16. The specific recognition of CRISPR/Cas12a to HPV-16 made the biosensor have good selectivity, while the TDN-modified sensing interface could reduce the cleaving steric resistance and improve the cleaving performance of CRISPR/Cas12a. In addition, the pretreated biosensor could complete sample detection within 100 min with a detection limit of 8.86 fM, indicating that the developed biosensor possesses the potential application prospect for fast and sensitive nucleic acid detection.

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Programmable readout sensor for microRNA: CRISPR/Cas12a-assisted multi-amplification strategy activated photoelectrochemistry-colorimetry detection

Cited by 14 publications

References 31 publications

Application of CRISPR/Cas Technology in Biological Nano‐Analysis

Application of CRISPR/Cas Technology in Biological Nano‐Analysis

Self-Powered Biosensor Based on DNA Walkers for Ultrasensitive MicroRNA Detection

Sensitive and Amplification-Free Electrochemiluminescence Biosensor for HPV-16 Detection Based on CRISPR/Cas12a and DNA Tetrahedron Nanostructures

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