Programmable nanoassembly consisting of two hairpin-DNAs for p53 gene determination

Xu, Jianguo; Wu, Zai‐Sheng; Shen, Weiyu; Le, Jingqing; Zheng, Tingting; Li, Hongling; Lee, Jia

doi:10.1016/j.bios.2017.03.052

Cited by 24 publications

(7 citation statements)

References 38 publications

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“…Highly sensitive detection of nucleic acid analytes is always pursued by researchers to accommodate the ever-increasing demands in disease diagnosis, environmental and food monitoring, and forensic identification (Debouck and Goodfellow, 1999 ; Liu et al, 2008 ; Zhang et al, 2013 ; Chen et al, 2019a ). Polymerase chain reaction is the most commonly used method for the detection of low amounts of nucleic acids (Asiello and Baeumner, 2011 ; Xu et al, 2017 ), but the delicate temperature control by a relatively expensive thermal cycler and the complex primer design limit its wide applications, especially in some resource-constrained regions. Compared with it, biosensor technology is especially attractive for bioanalysis due to its potential advantages including economy, portability, and facile operation.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive Electrochemical DNA Biosensor Fabrication by Coupling an Integral Multifunctional Zirconia-Reduced Graphene Oxide-Thionine Nanocomposite and Exonuclease I-Assisted Cleavage

Chen

Liu

et al. 2020

Front. Chem.

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In this work, a simple but sensitive electrochemical DNA biosensor for nucleic acid detection was developed by taking advantage of exonuclease (Exo) I-assisted cleavage for background reduction and zirconia-reduced graphene oxide-thionine (ZrO 2-rGO-Thi) nanocomposite for integral DNA recognition, signal amplification, and reporting. The ZrO 2-rGO nanocomposite was obtained by a one-step hydrothermal synthesis method. Then, thionine was adsorbed onto the rGO surface, via π-π stacking, as an excellent electrochemical probe. The biosensor fabrication is very simple, with probe DNA immobilization and hybridization recognition with the target nucleic acid. Then, the ZrO 2-rGO-Thi nanocomposite was captured onto an electrode via the multicoordinative interaction of ZrO 2 with the phosphate group on the DNA skeleton. The adsorbed abundant thionine molecules onto the ZrO 2-rGO nanocomposite facilitated an amplified electrochemical response related with the target DNA. Since upon the interaction of the ZrO 2-rGO-Thi nanocomposite with the probe DNA an immobilized electrode may also occur, an Exo I-assisted cleavage was combined to remove the unhybridized probe DNA for background reduction. With the current proposed strategy, the target DNA related with P53 gene could be sensitively assayed, with a wide linear detection range from 100 fM to 10 nM and an attractive low detection limit of 24 fM. Also, the developed DNA biosensor could differentiate the mismatched targets from complementary target DNA. Therefore, it offers a simple but effective biosensor fabrication strategy and is anticipated to show potential for applications in bioanalysis and medical diagnosis.

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Section: Introductionmentioning

confidence: 99%

Ultrasensitive Electrochemical DNA Biosensor Fabrication by Coupling an Integral Multifunctional Zirconia-Reduced Graphene Oxide-Thionine Nanocomposite and Exonuclease I-Assisted Cleavage

Chen

Liu

et al. 2020

Front. Chem.

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“…The p53 gene is known also as tumor suppressor p53, cellular tumor antigen p53 or NY-CO-13 transforming-related protein 53 and it acts as a tumour suppressor and prevents mutation, so it is called a tumour suppressor gene and found on chromosome 17 [ 1 ]. The p53 gene plays an important role in the prevention of cancer initiation and formation, so most of the p53 gene is a mutated gene (>50%) [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

p53 Gene (NY-CO-13) Levels in Patients with Chronic Myeloid Leukemia: The Role of Imatinib and Nilotinib

2018

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The p53 gene is also known as tumor suppressor p53. The main functions of the p53 gene are an anticancer effect and cellular genomic stability via various pathways including activation of DNA repair, induction of apoptosis, and arresting of cell growth at the G1/S phase. Normally, the p53 gene is inactivated by mouse double minute 2 proteins (mdm2), but it is activated in chronic myeloid leukemia (CML). Tyrosine kinase inhibitors are effective chemotherapeutic agents in the management of CML. The purpose of the present study was to evaluate the differential effect of imatinib and nilotinib on p53 gene serum levels in patients with CML. A total number of 60 patients with chronic myeloid leukemia with ages ranging from 47 to 59 years were recruited from the Iraqi Hematology Center. They started with tyrosine kinase inhibitors as first-line chemotherapy. They were divided into two groups—Group A, 29 patients treated with imatinib and Group B, 31 patients treated with nilotinib—and compared with 28 healthy subjects for evaluation p53 serum levels regarding the selective effect of either imatinib or nilotinib. There were significantly (p < 0.01) high p53 gene serum levels in patients with CML (2.135 ± 1.44 ng/mL) compared to the control (0.142 ± 0.11 ng/mL). Patients with CML that were treated with either imatinib or nilotinib showed insignificant differences in most of the hematological profile (p > 0.05) whereas, p53 serum levels were high (3.22 ± 1.99 ng/mL) in nilotinib-treated patients and relatively low (1.18 ± 0.19 ng/mL) in imatinib-treated patients (p = 0.0001). Conclusions: Nilotinib is more effective than imatinib in raising p53 serum levels in patients with chronic myeloid leukemia.

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“…Because of the high conductivity and current density of graphene, researchers are interested in discovering a graphene-based conductive ink. 23,24 Due to the fact that pure graphene ink has low conductivity, it limits its widespread use. Therefore, mixing graphene nanosheets and Ag NPs together creates a hybrid ink that has high conductivity and low AgNPs concentration.…”

Section: Polymerase Chain Reaction (Pcr) Based Techniques Including Methylation-sensitive High-resolution Melting (Ms-hrm) Andmentioning

confidence: 99%

Reliable recognition ofDNAmethylation using bioanalysis of hybridization on the surface of Ag/GQDnanocomposite stabilized on poly (β‐cyclodextrin): A new platform forDNAdamage studies using genosensor technology

Aghaei

Saadati

Hasanzadeh

et al. 2021

J of Molecular Recognition

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Due to the role of DNA methylation in causing cancer in the present study, an innovative and inexpensive method was designed for the sensitive detection of DNA methylation. The silver-graphene quantum dots (Ag/GQDs) nano ink with high electrical conductivity was used as a substrate for genosensor fabrication toward identification of DNA hybridization. Also, poly (β-cyclodextrin) (p[β-CD]) has been used as a biointerface for the stabilization of Ag/GQD nano ink. The thiolated pDNA strand (5 0 -SH-TCCGCTTCCCGACCCGCACTCCGC-3 0 ) (as bioreceptor element) was fixed on the substrate and hybridized with methylated (5 0 -GC(M)GGAGTGC(M)GGGTC(M) GGGAAGC(M)GGA-3 0 ) and unmethylated (5 0 -GCGGAGTGCGGGTCGGGAAGCGGA-3 0 ) cDNAs, as target sequences were studied using electroanalysis methods. Under optimal conditions and using electrochemical techniques, the linear range was 1 AM to 1 PM with LLOQ of 1aM. Finally, the designed DNA genosensor was used for detection of DNA methylation in human plasma samples and can be used to detect methylation in patient samples. It is expected that the designed DNA-based biodevice will be used to early stage diagnosis of cancer using monitoring of DNA methylation. Also, this type of genosensor can be used for epigenetic studies in the near future.

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Programmable nanoassembly consisting of two hairpin-DNAs for p53 gene determination

Cited by 24 publications

References 38 publications

Ultrasensitive Electrochemical DNA Biosensor Fabrication by Coupling an Integral Multifunctional Zirconia-Reduced Graphene Oxide-Thionine Nanocomposite and Exonuclease I-Assisted Cleavage

Ultrasensitive Electrochemical DNA Biosensor Fabrication by Coupling an Integral Multifunctional Zirconia-Reduced Graphene Oxide-Thionine Nanocomposite and Exonuclease I-Assisted Cleavage

p53 Gene (NY-CO-13) Levels in Patients with Chronic Myeloid Leukemia: The Role of Imatinib and Nilotinib

Reliable recognition ofDNAmethylation using bioanalysis of hybridization on the surface of Ag/GQDnanocomposite stabilized on poly (β‐cyclodextrin): A new platform forDNAdamage studies using genosensor technology

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