Programmable modification of cell adhesion and zeta potential in silica microchips

Kirby, Brian J.; Wheeler, Aaron R.; Zare, Richard N.; Fruetel, Julia A.; Shepodd, Timothy J.

doi:10.1039/b210621n

Cited by 77 publications

(73 citation statements)

References 36 publications

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“…Hence, this technique may be useful for upcoming applications in microfluidic cell analysis. Some approaches involve reactive-coating of the inner surface of the microfluidic channels [157][158][159] with antibodies [160,161], with selectin (cell adhesion molecules) [162] or with enzymes [163]. These coating techniques use cell adhesion to enable trapping of cells with fairly low immobilization.…”

Section: Mechanical Manipulationmentioning

confidence: 99%

Polymer-Based Microfluidic Devices for Pharmacy, Biology and Tissue Engineering

2012

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This paper reviews microfluidic technologies with emphasis on applications in the fields of pharmacy, biology, and tissue engineering. Design and fabrication of microfluidic systems are discussed with respect to specific biological concerns, such as biocompatibility and cell viability. Recent applications and developments on genetic analysis, cell culture, cell manipulation, biosensors, pathogen detection systems, diagnostic devices, high-throughput screening and biomaterial synthesis for tissue engineering are presented. The pros and cons of materials like polydimethylsiloxane (PDMS), polymethylmethacrylate (PMMA), polystyrene (PS), polycarbonate (PC), cyclic olefin copolymer (COC), glass, and silicon are discussed in terms of biocompatibility and fabrication aspects. Microfluidic devices are widely used in life sciences. Here, commercialization and research trends of microfluidics as new, easy to use, and cost-effective measurement tools at the cell/tissue level are critically reviewed.

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Section: Mechanical Manipulationmentioning

confidence: 99%

Polymer-Based Microfluidic Devices for Pharmacy, Biology and Tissue Engineering

2012

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“…The wafers were then rinsed with DI water and dried in nitrogen. Prior to membrane fabrication, the glass channels were coated with an acrylate-terminated self-assembling monolayer to enable covalent attachment of the polyacrylamide membrane to the channel walls (Kirby et al 2003;Hjerten 1967Hjerten , 1985. For this, the channels were prepared by exposing to 1 M HCl for 30 min, rinsing in DI water, and then exposing to 1 M NaOH for 30 min.…”

Section: Membrane Fabrication and Surface Treatmentmentioning

confidence: 99%

“…Finally, the channels were coated with linear polyacrylamide to suppress the electroosmotic flow (Kirby et al 2003;Hjerten 1967Hjerten , 1985. The channels were filled with a degassed solution of 50 mg/ml acrylamide in deionized water containing 250 ppm hydroquinone and 2 mg/ml V-50 photoinitiator and exposed to UV light in a UV oven for 30 min.…”

Section: Membrane Fabrication and Surface Treatmentmentioning

confidence: 99%

Integrated microfluidic preconcentrator and immunobiosensor

Kondapalli

Connelly

Baeumner

et al. 2011

Microfluid Nanofluid

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We present a microfluidic biosensor that integrates membrane-based preconcentration with fluorescence detection. The concentration membrane was fabricated in polyacrylamide by an in situ photopolymerization technique at the junction of glass microchannels. Liposomes entrapping sulforhodamine B dye molecules were used for signal amplification. The biotin-streptavidin binding system was a model system for evaluating device performance. Biotinylated liposomes were preconcentrated at the membrane by applying an electric field across the membrane. The electric field causes the liposomes to migrate toward the membrane where they are concentrated by a sieving effect. Two orders of magnitude concentration was achieved after applying the electric field for only 2 min. The concentrated bolus was then eluted toward the detection unit, where the biotinylated liposomes were captured by immobilized streptavidin. The integrated system with the preconcentration module shows a 14-fold improvement in signal as opposed to a system that does not include preconcentration.

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“…Next, a layer of NeutrAvidin was covalently attached to the surface by incubating the surface for 60 minutes with 25 micrograms of NeutrAvidin per milliliter of PBS. Finally, the biotinylated monoclonal antibody was immobilized on the surface via the biotinNeutrAvidin bond (Liu, Moy et al 1997;Kirby, Wheeler et al 2003;Gleghorn, Pratt et al 2010). Devices were stored before use in a 1% (m/v) BSA in PBS solution for up to two hours.…”

Section: Microdevice Functionalizationmentioning

confidence: 99%

Immunocapture of prostate cancer cells with anti-PSMA antibodies in microdevices

Santana

Liu

Bander

et al. 2011

2011 IEEE 37th Annual Northeast Bioengineering Conference (NEBEC)

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Patients suffering from cancer can shed tumor cells into the bloodstream, leading to one of the most important mechanisms of metastasis. As such, the capture of these cells is of great interest. Circulating tumor cells are typically extracted from circulation through positive selection with the epithelial cell-adhesion molecule (EpCAM), leading to currently unknown biases when cells are undergoing epithelial-to-mesenchymal transition. For prostate cancer, prostate-specific membrane antigen (PSMA) presents a compelling target for immunocapture, as PSMA levels increase in higher-grade cancers and metastatic disease and are specific to the prostate epithelium. This study uses monoclonal antibodies J591 and J415-antibodies that are highly specific for intact extracellular domains of PSMA on live cells-in microfluidic devices for the capture of LNCaPs, a PSMA-expressing immortalized prostate cancer cell line, over a range of concentrations and shear stresses relevant to immunocapture. Our results show that J591 outperforms J415 and a mix of the two for prostate cancer capture, and that capture performance saturates following incubation with antibody concentrations of 10 micrograms per milliliter.

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Programmable modification of cell adhesion and zeta potential in silica microchips

Cited by 77 publications

References 36 publications

Polymer-Based Microfluidic Devices for Pharmacy, Biology and Tissue Engineering

Polymer-Based Microfluidic Devices for Pharmacy, Biology and Tissue Engineering

Integrated microfluidic preconcentrator and immunobiosensor

Immunocapture of prostate cancer cells with anti-PSMA antibodies in microdevices

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