Programmable Microparticle Array for In Situ Modification and Multiple miRNA Detection

Abstract

Simultaneous detection of multiple miRNAs of one disease can greatly reduce misdiagnosis and improve the detection rate, which is helpful for early cancer diagnosis. Here, a programmable microparticle-array-based acoustic microchip for in situ simultaneous multiple miRNAs detection is developed. On this microchip, the multiple probes-labeled microparticle array can be procedurally arranged in a microfluidic reaction chamber when four orthogonally piezoelectric transducers are applied. The probes-labeled microparticle array offers a platform for full molecular contact under dynamic ultrasonic streaming, and the array supplies a multipoint data correction to reduce the false positive of the detection results for more precisely visible fluorescence multiple target miRNAs sensing. We employed miRNA-21, miRNA-210, and miRNA-155 as specific biomarkers of pancreatic cancer and successfully finished the multiple miRNAs simultaneous detection in the microchip with a detection limit of 139.1, 179.9, and 111.4 pM, respectively. Such a device is programmable by adjusting the imputing frequency and voltage, and target biomarkers can be easily collected when the ultrasound force is released for further analysis, which shows great potential in multiple miRNAs enrichment and simultaneous detection for cancer clinical diagnosis.

Acoustic Trapping: An Emerging Tool for Microfabrication Technology

“…[38,100,112,131,[135][136][137][138][139][140][141][142][143][144][145][146][147][148][149][150][151][152] Through the construction of 1D or 2D multinodes acoustic field, SAW or BAW-based acoustic trapping devices can arrange multiple biological samples at the same time in well-defined 1D or 2D patterns, which enables high-throughput scree ning. [39,102,104,[118][119][120][121][122][123][124][125][126][127][128][129][130][131][132] For instance, Tian et al showed that a periodic defect-free 2D microarray of coacervate microdroplets could be formed in an acoustic standing wave pressure field, which could be further functionalized by spontaneously sequestering dyes, enzymes, and particles. The chemically encoded 2D droplet arrays displayed spatial and time-dependent fluorescence outputs when exposed to a reactant diffusion gradient, and could dynamically sense encoded information in the advancing reaction-diffusion front (Figure 9A-C).…”

“…Through the construction of 1D or 2D multinodes acoustic field, SAW or BAW‐based acoustic trapping devices can arrange multiple biological samples at the same time in well‐defined 1D or 2D patterns, which enables high‐throughput screening. [ 39,102,104,118–132 ] For instance, Tian et al. showed that a periodic defect‐free 2D microarray of coacervate microdroplets could be formed in an acoustic standing wave pressure field, which could be further functionalized by spontaneously sequestering dyes, enzymes, and particles.…”

“…holography, and traveling wave fields have given rise to many potential applications in diverse disciplines (Table 1). For instance, due to the rapid deposition of objects ranging from microns to millimeters in size and various shaped 1D and 2D configurations, SAWs and BAWs-based acoustic trapping devices have been used in chemical/biochemical sen sing [39,102,104,[118][119][120][121][122][123][124][125][126][127][128][129][130][131][132] and materials science. [103,[133][134][135] These relatively simple SAW or BAW-based acoustic trapping devices have been widely used to immobilize microparticles in symmetrical arrangements, such as 1D lines, 2D squares, or 3D cube patterns.…”

Advancements in artificial micro/nanomotors for nucleic acid biosensing: a review of recent progress

“…4k). 76 Through precise adjustment of the input frequency and voltage, a highly specific micromotor array was rapidly formed within the chamber, providing abundant reaction sites. The acoustic streaming further enhanced the contact between probes, facilitating subsequent modification processes.…”

Droplet Microfluidic-Based Low-Cost and High-Speed Microsphere Array Direct Writing Technology and Its Applications

“…In recent years, microsphere arrays have been developed for various applications, including microlens arrays, super-resolution imaging, , advanced information storage, anti-counterfeiting, microsphere array electrodes, electrocatalysis, electrochemical capacitors, cell culture, and even the specific identification of a wide range of analytes. , Interestingly, the artificial electric organs that power implanted electronic devices, wearables, and other mobile devices also rely on constructed arrays of microspheres . In conclusion, microsphere arrays can drive scientific advances in optics, electronics, chemistry, biology, and other emerging fields and have become a potentially powerful and essential tool. − …”