Programmable DNA switches and their applications

Harroun, Scott G.; Prévost-Tremblay, Carl; Lauzon, Dominic; Desrosiers, Arnaud; Wang, Xiaomeng; Pedro, L; Vallée‐Bélisle, Alexis

doi:10.1039/c7nr07348h

Cited by 106 publications

(66 citation statements)

References 303 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such differences in environmental characteristics could serve as another type of target for nanocarriers to aim for. In order to arm pristine DNA nanostructures with stimuli responsive capability, dynamic elements need to be integrated into the designed nanocarrier ( Figure 5A) (Harroun et al, 2018;Zhang Y. et al, 2019). In the following section, we discuss some mechanisms that may be incorporated into DNA nanocarriers to realize environmental responsive cargo delivery.…”

Section: Dna Nanocarriers Responding To Microenvironmentsmentioning

confidence: 99%

Rationally Designed DNA Nanostructures for Drug Delivery

Xia

Wang

2020

Front. Chem.

View full text Add to dashboard Cite

DNA is an excellent biological material that has received growing attention in the field of nanotechnology due to its unique capability for precisely engineering materials via sequence specific interactions. Self-assembled DNA nanostructures of prescribed physicochemical properties have demonstrated potent drug delivery efficiency in vitro and in vivo. By using various conjugation techniques, DNA nanostructures may be precisely integrated with a large diversity of functional moieties, such as targeting ligands, proteins, and inorganic nanoparticles, to enrich their functionalities and to enhance their performance. In this review, we start with introducing strategies on constructing DNA nanostructures. We then summarize the biological barriers ahead of drug delivery using DNA nanostructures, followed by introducing existing rational solutions to overcome these biological barriers. Lastly, we discuss challenges and opportunities for DNA nanostructures toward real applications in clinical settings.

show abstract

Section: Dna Nanocarriers Responding To Microenvironmentsmentioning

confidence: 99%

Rationally Designed DNA Nanostructures for Drug Delivery

Xia

Wang

2020

Front. Chem.

View full text Add to dashboard Cite

show abstract

“…Engineering complex self-regulation mechanisms using simple molecular assembly provides a quantitative and programmable chemical strategy to develop and optimize nanosystems with applications ranging from biosensing 49 to chemical computing 50,51 and drug delivery 52,53 . For example, current strategies to extend the dynamic range of sensors consist of combining two or multiple sensors with different affinities [54][55][56] . In contrast, here, we have illustrated how a three-component sensing system can be programmed to display either a narrow or an extended dynamic range.…”

Section: Discussionmentioning

confidence: 99%

Programming complex regulation mechanisms through simple molecular assembly

Lauzon

Vallée‐Bélisle

2020

Preprint

Self Cite

View full text Add to dashboard Cite

What are the advantages and disadvantages of building nanosystems using one or multiple components? More than 55% of all proteins found in living organisms are multimeric and likely exploit molecular assembly to create new functional entities. However, the specific contribution of molecular assembly to the creation of novel functions remains relatively unexplored at the thermodynamic, kinetic and molecular levels. Here, we use theory and a simple experimental model to determine the design rules for engineering efficient self-assembled, self-regulated nanosystems. Using these rules, we have rationally designed and implemented various regulation mechanisms (e.g., cooperative and anticooperative assembly, self-inhibition, molecular timer) into two model trimeric nanosystems including a complex artificial catalyst. These simple strategies based on molecular assembly have been extensively exploited by natural biosystems and are expected to play a crucial role in the development of future self-regulated nanotechnologies.

show abstract

“…In this case, the toehold structure drives multiple new capabilities for DNA storage: 1) it increases the theoretical information density and capacity of DNA storage by inhibiting non-specific binding within data payloads; 2) it enables transcription-based, non-destructive information access; and 3) it makes possible in-storage file operations. We envision other new architectures and capabilities may be on the horizon given rapid advances in DNA origami 26 , molecular handles [27][28][29] , and molecular manipulations developed in fields such as synthetic biology 30 .…”

Section: Doris Represents a Proof Of Principle Framework For How Inclmentioning

confidence: 99%

Dynamic DNA-based information storage

Lin

Tuck

Keung

2019

Preprint

View full text Add to dashboard Cite

Technological leaps are often driven by key innovations that transform the underlying architectures of systems. Current DNA storage systems largely rely on polymerase chain reaction, which broadly informs how information is encoded, databases are organized, and files are accessed. Here we show that a hybrid 'toehold' DNA structure can unlock a fundamentally different, dynamic DNA-based information storage system architecture with broad advantages.This innovation increases theoretical storage densities and capacities by eliminating non-specific DNA-DNA interactions common in PCR and increasing the encodable sequence space. It also provides a physical handle with which to implement a range of in-storage file operations. Finally, it reads files non-destructively by harnessing the natural role of transcription in accessing information from DNA. This simple but powerful toehold structure lays the foundation for an information storage architecture with versatile capabilities.

show abstract

Programmable DNA switches and their applications

Cited by 106 publications

References 303 publications

Rationally Designed DNA Nanostructures for Drug Delivery

Rationally Designed DNA Nanostructures for Drug Delivery

Programming complex regulation mechanisms through simple molecular assembly

Dynamic DNA-based information storage

Contact Info

Product

Resources

About