Programmable aperture microscopy: A computational method for multi-modal phase contrast and light field imaging

Zuo, Chao; Sun, Jiasong; Feng, Shijie; Zhang, Minliang; Chen, Qian

doi:10.1016/j.optlaseng.2015.12.012

Cited by 40 publications

(24 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In FPM, the specimen is successively illuminated by plane waves from different angles with a programmable LED array, and the corresponding lowresolution intensity images are synthesized in the Fourier domain to reconstruct a high-resolution wide-field complex (both amplitude and phase) image. Light-field microscopy is a motion-free single-shot 3D microscopy technique, which is achieved by inserting a microlens array in the intermediate image plane just before the camera sensor, allowing for recording a four-dimensional (4D) light field containing both the intensity and angular distribution of all rays [84,85,86]. During the reconstruction phase, ray-tracing techniques can be used to reconstruct synthetic photographs, estimate depth, and change focus or viewing perspectives.…”

Section: Phase Changesmentioning

confidence: 99%

“…For example, the spatial resolution (mainly including lateral/axial resolution and de-pixelation) is associated with the illumination wavelength, illumination angle, objective numerical aperture (NA), and the pixel size of the detector. For phase retrieval, illumination wavelength [156,174], angle [79,80], aperture modulation [76,86], sensor defocus [168] can all produce phase contrast that allows for non-interferometic quantitative phase recovery. Therefore, if one wants to design a quantitative phase microscopy system for high-resolution single-cell-level labelfree imaging, all these factors should be considered comprehensively to develop an appropriate optical modulation scheme.…”

Section: Computational Light Microscopy: Conceptmentioning

confidence: 99%

“…During the reconstruction phase, ray-tracing techniques can be used to reconstruct synthetic photographs, estimate depth, and change focus or viewing perspectives. Programmable aperture microscopy is a multi-modal microscopy technique by integrating a programmable light modulation device [e.g., spatial light modulator (SLM) [87,88] or programmable LCD [86]] into a conventional wide-field microscope. By selectively modulating the light distribution at the objective aperture plane, numerous imaging modalities, such as bright field, dark field, DPC, QPI, multi-perspective imaging, and full-resolution light-field imaging to be achieved and switched rapidly, without requiring specialized hardware and any physically moving parts.…”

Section: Phase Changesmentioning

confidence: 99%

“…Computational light microscope brought a revolution of the microscope in diversification, flexibility, and convenience of imaging methods. The active illumination control (LED or LCD) were introduced into the microscope system to replace the traditional aperture diaphragm, realizing so-called "computational illumination" that gains the flexibility to produce sophisticated illumination patterns or dynamically switchable illumination sources with no physically moving parts [75,77,78,86,103,215,216]. In a microscopic system, the NA of the objective lens is a very important parameter because it determines the maximum diffraction angle that the microscope can receive.…”

Section: Principle and Algorithmsmentioning

confidence: 99%

See 3 more Smart Citations

Smart Computational Light Microscopes (SCLMs) of Smart Computational Imaging Laboratory (SCILab)

Fan

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Computational microscopy, as a subfield of computational imaging, combines optical manipulation and image algorithmic reconstruction to recover multi-dimensional microscopic images or information of micro-objects. In recent years, the revolution in light-emitting diodes (LEDs), low-cost consumer image sensors, modern digital computers, and smartphones provide fertile opportunities for the rapid development of computational microscopy. Consequently, diverse forms of computational microscopy have been invented, including digital holographic microscopy (DHM), transport of intensity equation (TIE), differential phase contrast (DPC) microscopy, lens-free on-chip holography, and Fourier ptychographic microscopy (FPM). These computational microscopy techniques not only provide high-resolution, label-free, quantitative phase imaging capability but also decipher new and advanced biomedical research and industrial applications. Nevertheless, most computational microscopy techniques are still at an early stage of "proof of concept" or "proof of prototype" (based on commercially available microscope platforms). Translating those concepts to stand-alone optical instruments for practical use is an essential step for the promotion and adoption of computational microscopy by the wider bio-medicine, industry, and education community. In this paper, we present four smart computational light microscopes (SCLMs) developed by our laboratory, i.e., smart computational imaging laboratory (SCILab) of Nanjing University of Science and Technology (NJUST), China. These microscopes are empowered by advanced computational microscopy techniques, including digital holography, TIE, DPC, lensless holography, and FPM, which not only enables multi-modal contrast-enhanced observations for unstained specimens, but also can recover their three-dimensional profiles quantitatively. We introduce their basic principles, hardware configurations, reconstruction algorithms, and software design, quantify their imaging performance, and illustrate their typical applications for cell analysis, medical diagnosis, and microlens characterization.

show abstract

Section: Phase Changesmentioning

confidence: 99%

Section: Computational Light Microscopy: Conceptmentioning

confidence: 99%

Section: Phase Changesmentioning

confidence: 99%

Section: Principle and Algorithmsmentioning

confidence: 99%

See 2 more Smart Citations

Smart Computational Light Microscopes (SCLMs) of Smart Computational Imaging Laboratory (SCILab)

Fan

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Some other asymmetric illumination methods could produce the phase contrast in the in-focus intensity image by break the symmetry of S (u) or P (u), and the prominent examples are differential phase contrast microscopy 36,40 and partitioned or programmable aperture microscopy. 41,42 The defocusing of optical system along z axial, which is another more convenient way to produce phase contrast and an imaginary part, would be introduced into the pupil function:…”

Section: Wotf For Axisymmetric Oblique Sourcementioning

confidence: 99%

Efficient quantitative phase microscopy using programmable annular LED illumination

Chen

Zhang

et al. 2017

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

Abstract:In this work, we present an efficient quantitative phase imaging (QPI) approach using programmable annular LED illumination. As a new type of coded light source, the LED array provides flexible illumination control for noninterferometric QPI based on a traditional microscopic configurations. The proposed method modulates the transfer function of system by changing the LED illumination pattern, which provides noise-robust response of transfer function and achieves twice resolution limit of objective NA. The quantitative phase can be recovered from slightly defocused intensity images through inversion of transfer function. Moreover, the weak object transfer function (WOTF) of axis-symmetric oblique source is derived, and the noise-free and noisy simulation results validate the predicted theory. Finally, we experimentally confirm accurate and repeatable performance of our method by imaging calibrated phase samples and cellular specimens with different NA objectives.

show abstract

Isotropic differential phase contrast microscopy for quantitative phase bio‐imaging

Chen

Lin

Luo

2018

Journal of Biophotonics

View full text Add to dashboard Cite

Quantitative phase imaging (QPI) has been investigated to retrieve optical phase information of an object and applied to biological microscopy and related medical studies. In recent examples, differential phase contrast (DPC) microscopy can recover phase image of thin sample under multi-axis intensity measurements in wide-field scheme. Unlike conventional DPC, based on theoretical approach under partially coherent condition, we propose a new method to achieve isotropic differential phase contrast (iDPC) with high accuracy and stability for phase recovery in simple and high-speed fashion. The iDPC is simply implemented with a partially coherent microscopy and a programmable thin-film transistor (TFT) shield to digitally modulate structured illumination patterns for QPI. In this article, simulation results show consistency of our theoretical approach for iDPC under partial coherence. In addition, we further demonstrate experiments of quantitative phase images of a standard micro-lens array, as well as label-free live human cell samples.

show abstract

Programmable aperture microscopy: A computational method for multi-modal phase contrast and light field imaging

Cited by 40 publications

References 28 publications

Smart Computational Light Microscopes (SCLMs) of Smart Computational Imaging Laboratory (SCILab)

Smart Computational Light Microscopes (SCLMs) of Smart Computational Imaging Laboratory (SCILab)

Efficient quantitative phase microscopy using programmable annular LED illumination

Isotropic differential phase contrast microscopy for quantitative phase bio‐imaging

Contact Info

Product

Resources

About