Programing stimuli-responsiveness of gelatin with electron beams: basic effects and development of a hydration-controlled biocompatible demonstrator

Riedel, Stefanie; Heyart, Benedikt; Apel, Katharina S.; Mayr, Stefan

doi:10.1038/s41598-017-17734-y

Cited by 13 publications

(11 citation statements)

References 20 publications

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“…The target cells used in the ADCC activity determination assay for the antiviral antibodies, unlike the ready-to-use tumor cell lines used for antitumor antibodies, were prepared by transient infection of the susceptible cells with live viruses or pseudoviruses 52 – 54 , so the preparation procedure is complex, and the target cells are unstable and highly variable. In this study, we generated a 293T/MERS cell line stably expressing the MERS-S as the target cell in the ADCC assay (Fig.…”

Section: Discussionmentioning

confidence: 99%

Development of an antibody-dependent cellular cytotoxicity reporter assay for measuring anti-Middle East Respiratory Syndrome antibody bioactivity

Cao

Wang

et al. 2020

Sci Rep

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Middle East Respiratory Syndrome coronavirus (MERS-CoV) is a highly virulent pathogen that causes Middle East Respiratory Syndrome (MERS). Anti-MERS-CoV antibodies play an integral role in the prevention and treatment against MERS-CoV infections. Bioactivity is a key quality attribute of therapeutic antibodies, and high accuracy and precision are required. The major methods for evaluating the antiviral effect of antiviral antibodies include neutralization assays using live viruses or pseudoviruses are highly variable. Recent studies have demonstrated that the antibody-dependent cellular cytotoxicity (ADCC) activity of antiviral antibodies is more consistent with the virus clearance effect in vivo than neutralization activity. However, no reports evaluating the ADCC activity of anti-MERS antibodies have been published to date. Here, we describe the development of a robust and reliable cell-based reporter gene assay for the determination of ADCC activity of anti-MERS antibodies using 293T/MERS cells stably expressing the spike protein of MERS-CoV (MERS-S) as target cells and the engineered Jurkat/NFAT-luc/FcγRIIIa stably expressing FcγRIIIA and NFAT reporter gene as effector cells. According to the ICH-Q2 analytical method guidelines, we carefully optimized the experimental conditions and assessed the performance of our assay. In addition, we found that the ADCC activity of afucosylated anti-MERS antibodies is higher than their fucosylated counterparts. The establishment of this ADCC determination system provides a novel method for evaluating the bioactivity of anti-MERS antibodies and improving ADCC activity through modification of N-glycosylation of the Fc segment.

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Section: Discussionmentioning

confidence: 99%

Development of an antibody-dependent cellular cytotoxicity reporter assay for measuring anti-Middle East Respiratory Syndrome antibody bioactivity

Cao

Wang

et al. 2020

Sci Rep

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“…The latter shows the minimum swelling ratio at the isoelectric point near the neutral pH (pH 7-9, type A, form porcine skin), where anionic and cationic forms are exactly balanced resulting in chain collapse. 40,76 In contrast, PGSG copolymers showed the minimum swelling at the acidic pH and the maximum was achieved at the basic pH. It can be concluded that the number of ionisable carboxyl groups is higher than that of amine groups in PGSG copolymers.…”

Section: Figmentioning

confidence: 93%

Elastomeric and pH-responsive hydrogels based on direct crosslinking of the poly(glycerol sebacate) pre-polymer and gelatin

Yoon

Chen

2018

Polym. Chem.

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“…They showed that the irradiation modifies the swelling properties of gelatine, which produces anisotropic deformation when used in a bilayer configuration (Figure 10B). [161] The structural design of pH-responsive actuators goes beyond layered structures. For example, researchers sought to emulate the mechanical properties and flexibility of yarn.…”

Section: Ionization Of Natural Acidic and Basic Polymersmentioning

confidence: 99%

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confidence: 99%

Materials, Actuators, and Sensors for Soft Bioinspired Robots

et al. 2020

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This review covers recent advancements in the field of bioinspired soft robotics, with a primary focus on the last 4 years (2017)(2018)(2019)(2020). The review serves as a toolbox for an interdisciplinary audience interested in the most recent bioinspired soft robotic technologies. In particular, it highlights and explores the vital components of soft robots, focusing on the enabling mechanisms and their biological inspirations. The first section discusses the materials used to fabricate soft bio inspired robots. Soft bioinspired actuation and sensing are then discussed, exploring their capabilities and implementation by researchers. Existing challenges and future potentials of bioinspired soft robots are addressed in the concluding remarks. This review provides engineers and scientists with the latest technological advancements and information needed for designing and developing the next generation of soft bioinspired robotic systems. The future applications of these robots will be grand and limitless. Materials Used for Bioinspired Sensors and ActuatorsClassical robotic systems are comprised of rigid bodies, actuators, and sensors. Unfortunately, many of these well-developed actuators and sensors are not transferable to soft bodies. Thus, researchers working in soft robotics need to reinvent actuators and sensors for soft moving bodies. Biological organisms can be an excellent inspiration for designing these soft actuators and sensors, allowing for their integration in both soft and rigid bodies. The design process of soft actuators and sensors have to be initiated with material selection and composition, for they are foundations upon which the actuators and sensors will be built around. Presently, a diversified list of materials has been used in the development of soft robotic systems. This section will cover some of the latest advancements in the last 4 years in the area of material selection and composition for the design and development of soft bioinspired actuators and sensors.During the past 4 years, researchers have produced soft bioinspired actuators and sensors using biological material such as muscle tissue, [23,24] and plant fibers; [25] carbon-based materials such as graphite and graphene oxide (GO) [26][27][28][29][30][31] and carbon nanotubes (CN); [32,33] hydrogel materials such as poly(Nisopropylacrylamide) (PNIPAM), [34,35] liquid crystal elastomers (LCE), [36] dielectric elastomers (DE), [37] and ionic polymermetal composites (IPMC). [38] An overview of these materials (Figure 1), along with their underlying mechanisms, are discussed below.Biological systems can perform complex tasks with high compliance levels. This makes them a great source of inspiration for soft robotics. Indeed, the union of these fields has brought about bioinspired soft robotics, with hundreds of publications on novel research each year. This review aims to survey fundamental advances in bioinspired soft actuators and sensors with a focus on the progress between 2017 and 2020, providing a primer for the materials used i...

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Programing stimuli-responsiveness of gelatin with electron beams: basic effects and development of a hydration-controlled biocompatible demonstrator

Cited by 13 publications

References 20 publications

Development of an antibody-dependent cellular cytotoxicity reporter assay for measuring anti-Middle East Respiratory Syndrome antibody bioactivity

Development of an antibody-dependent cellular cytotoxicity reporter assay for measuring anti-Middle East Respiratory Syndrome antibody bioactivity

Elastomeric and pH-responsive hydrogels based on direct crosslinking of the poly(glycerol sebacate) pre-polymer and gelatin

Materials, Actuators, and Sensors for Soft Bioinspired Robots

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