Prognostic value of wingless-type proteins in non-small cell lung cancer patients: a meta-analysis

Jin, Jiajia; Zhan, Ping; Qian, Hong; Wang, Xiaoxia; Katoh, Masaru; Phan, Kevin; Chung, Jin-Haeng; Lv, Tangfeng; Song, Yong

doi:10.21037/tlcr.2016.08.08

Cited by 13 publications

(10 citation statements)

References 37 publications

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“…24 A prior meta-analysis illustrated that overexpression of Wnt proteins (Wnt1 and Wnt5a) was associated with unsatisfactory overall survival in patients suffering from lung cancer. 29 In addition, another study confirmed that PRC1, a target of the Wnt signaling pathway, is vital for the Wnt pathway to regulate oncogenesis and the proliferation of CSCs and it acts as the upper stream of β-catenin. 24 To conclude, our present study revealed that PRC1 gene silencing exerts an inhibitory function over proliferation, and angiogenesis of retinoblastoma cells by decreasing the expressions of VEGF, Wnt1, β-catenin, and CyclinD1.…”

Section: Discussionmentioning

confidence: 95%

Retracted: PRC1 gene silencing inhibits proliferation, invasion, and angiogenesis of retinoblastoma cells through the inhibition of the Wnt/β‐catenin signaling pathway

Liao

Yin

Deng

et al. 2019

J of Cellular Biochemistry

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Retinoblastoma is an ocular malignancy occurring in childhood. The current study evaluates the ability of silenced PRC1 on retinoblastoma cell proliferation, and angiogenesis via the Wnt/β‐catenin signaling pathway. A total of 36 cases of retinoblastoma tissues (n = 36) and normal retinal tissues (n = 10) were selected in the current study. Retinoblastoma cells presenting with the high PRC1 messenger RNA (mRNA) expression were selected among the WERI‐Rb‐1, HXO‐RB44, Y79, SO‐Rb50, and SO‐Rb70 cells lines, and were transfected with siRNA‐PRC1 and LiCl (the activator of the Wnt/β‐catenin pathway). The expressions of PRC1, VEGF, Wnt1, β‐catenin, CyclinD1, extent of β‐catenin, and GSK‐3β phosphorylation were evaluated. Cell proliferation, cell‐cycle distribution, and cell invasion of retinoblastoma cells were evaluated by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay, flow cytometry, and Transwell assay. The angiogenesis of retinoblastoma cells was detected by tube formation assay. HXO‐RB44 and WERI‐Rb‐1 cells were selected owing to the highest PRC1 mRNA expression. Meanwhile, PRC2 gene silencing presented lower expression levels of PRC1, VEGF, Wnt1, β‐catenin, CyclinD1, extent of β‐catenin and GSK‐3β phosphorylation, decreased proliferation and invasion abilities, extended G0/G1 phase, and shortened S and G2/M phases of HXO‐RB44 and WERI‐Rb‐1 cells, suggesting the silenced PRC2 inactivated Wnt/β‐catenin pathway, so as to further restrain the retinoblastoma cell proliferation, invasion, and angiogenesis. These results support the view that PRC1 gene silencing could suppress the proliferation, and angiogenesis of retinoblastoma cells by repressing the Wnt/β‐catenin pathway.

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Section: Discussionmentioning

confidence: 95%

Retracted: PRC1 gene silencing inhibits proliferation, invasion, and angiogenesis of retinoblastoma cells through the inhibition of the Wnt/β‐catenin signaling pathway

Liao

Yin

Deng

et al. 2019

J of Cellular Biochemistry

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“…The Wnt/β-catenin pathway plays a critical role in cancer initiation, development, and metastasis in various human cancer types, including lung cancer [7,8,9,10,11]. In lung cancer, dysregulation of Wnt signaling has been found [38], and overexpression of Wnt proteins (Wnt1 and Wnt5a) was notably linked to unfavorable overall survival in lung cancer patients [39]. In addition, constitutive Wnt pathway activation is associated with tumor recurrence and poor prognosis in NSCLC patients [9,40,41,42,43,44,45].…”

Section: Discussionmentioning

confidence: 99%

Emetine Synergizes with Cisplatin to Enhance Anti-Cancer Efficacy against Lung Cancer Cells

Chang

Shih

et al. 2019

IJMS

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Cisplatin is still the primary therapeutic choice for advanced lung cancers without driver mutations. The occurrence of cisplatin resistance is a major clinical problem in lung cancer treatment. The natural extracted agent emetine reportedly has anticancer effects. This study aimed to explore the possible role of emetine in cisplatin resistance. We used cell viability, Western blot, and Wnt reporter assays to show that emetine suppresses proliferation, β-catenin expression, and Wnt/β-catenin signaling in non-small cell lung cancer (NSCLC). The synergism of emetine and cisplatin was assessed by constructing isobolograms and calculating combination index (CI) values using the Chou-Talalay method. Emetine effectively synergized with cisplatin to suppress the proliferation of cancer cells. Furthermore, nuclear β-catenin and cancer stem cell-related markers were upregulated in the cisplatin-resistant subpopulation of CL1-0 cells. Emetine enhanced the anticancer efficacy of cisplatin and synergized with cisplatin in the cisplatin-resistant subpopulation of CL1-0 cells. Taken together, these data suggest that emetine could suppress the growth of NSCLC cells through the Wnt/β-catenin pathway and contribute to a synergistic effect in combination with cisplatin.

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“…Wnt/β-catenin signaling is tightly regulated at multiple cellular levels and is dysregulated in lung cancer [ 31 ]. Our prior meta-analysis [ 32 ] demonstrated that the overexpression of Wnt proteins (Wnt1 and Wnt5a) was notably linked to unfavorable overall survival in lung cancer patients. Chen et al [ 11 ] discovered that PRC1 interacts with the β-catenin destruction complex, controls Wnt3a-induced membrane sequestration of the damaging complex, limits adenomatous polyposis coli (APC) durability, and encourages β-catenin release from the APC complex.…”

Section: Discussionmentioning

confidence: 99%

PRC1 contributes to tumorigenesis of lung adenocarcinoma in association with the Wnt/β-catenin signaling pathway

Zhan

Zhang

et al. 2017

Mol Cancer

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BackgroundProtein regulator of cytokinesis-1 (PRC1) belongs to the microtubule-associated proteins (MAPs) family, and is involved in cytokinesis. Recent investigations suggest PRC1 involvement in human carcinogenesis, including breast carcinoma, hepatocellular carcinoma and etc. However, whether PRC1 contributes to lung adenocarcinoma tumorigenesis remains unknown.MethodsQuantitative reverse-transcription polymerase chain reaction (qRT-PCR), Western blotting and Immunohistochemical staining (IHC) were used to evaluate and contrast the PRC1 expression profile in lung adenocarcinoma and adjacent normal lung tissues. We examined the clinical use of PRC1 in lung adenocarcinoma prognosis. Additionally, the tumorigenesis impact of PRC1 in lung adenocarcinoma cells was verified via in vitro and in vivo metastasis and tumorigenesis assays. Notably, Next Generation Sequencing (NGS) was performed to investigate the molecular mechanism underlying the oncogenic role of PRC1 in lung adenocarcinoma.ResultsPRC1 mRNA and protein expressions were upregulated in lung adenocarcinoma tissues compared to adjacent normal lung tissues. PRC1 protein overexpression correlated with lymph node metastasis and was an independent poor prognostic factor for lung adenocarcinoma patients. Our data implied that PRC1 depletion limited the proliferation and invasion of lung adenocarcinoma cells in vitro and lowered tumor development and lung metastasis in vivo. Remarkably, limiting PRC1 substantially prompted G2/M phase cell cycle arrest and apoptosis. Mechanistically, by conducting NGS on PRC1-depleted A549 cells and control cells, we discovered that PRC1 expression was significantly correlated with the Wnt signaling pathway.ConclusionsThis investigation offers confirmation that PRC1 is a prognostic and promising therapeutic biomarker for people with lung adenocarcinoma and takes on a key part in the activation of the Wnt/β-catenin pathway in lung adenocarcinoma development.Electronic supplementary materialThe online version of this article (doi:10.1186/s12943-017-0682-z) contains supplementary material, which is available to authorized users.

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Prognostic value of wingless-type proteins in non-small cell lung cancer patients: a meta-analysis

Cited by 13 publications

References 37 publications

Retracted: PRC1 gene silencing inhibits proliferation, invasion, and angiogenesis of retinoblastoma cells through the inhibition of the Wnt/β‐catenin signaling pathway

Retracted: PRC1 gene silencing inhibits proliferation, invasion, and angiogenesis of retinoblastoma cells through the inhibition of the Wnt/β‐catenin signaling pathway

Emetine Synergizes with Cisplatin to Enhance Anti-Cancer Efficacy against Lung Cancer Cells

PRC1 contributes to tumorigenesis of lung adenocarcinoma in association with the Wnt/β-catenin signaling pathway

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