Prognostic value of vascular endothelial growth factor-C and podoplanin mRNA expression in esophageal cancer

Juchniewicz, Agnieszka; Niklińska, Wiesława; Kowalczuk, Oksana; Laudański, Wojciech; Sulewska, Anetta; Dzięgielewski, Piotr; Milewski, Robert; Naumnik, Wojciech; Kozłowski, Mirosław; Nikliński, Jacek

doi:10.3892/ol.2015.3824

Cited by 9 publications

(6 citation statements)

References 23 publications

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“…However, in addition to lymphatics, these protein are expressed in various cell types and contribute to multiple molecular processes, including those in malignancies, as it has been demonstrated in a number of recent comprehensive reviews (54)(55)(56)(57)(58)(59)(60)(61)(62). This may provide an explanation for inconsistent data on the expression of analyzed proteins in cancer and their impact on tumor progression and clinical outcome (63)(64)(65)(66)(67)(68)(69)(70)(71)(72)(73)(74)(75)(76)(77).…”

Section: Discussionmentioning

confidence: 98%

Lymphatics-associated genes are downregulated at transcription level in non-small cell lung cancer

Kowalczuk

Laudański

et al. 2018

Oncol Lett

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The present study aimed to verify a possibility of ongoing lymphangiogenesis in non-small cell lung cancer (NSCLC) via examination of mRNA levels of a number of lymphangiogenesis-associated genes in tumors. It was hypothesized that transcriptional activation of these genes would occur in tumors that stimulate new lymphatic vessel formation. The study was performed on 140 pairs of fresh-frozen surgical specimens of cancer and unaffected lung tissues derived from NSCLC stage I-IIIA patients. mRNA levels were evaluated with the reverse transcription-quantitative polymerase chain reaction method and expressed as fold change differences between the tumor and normal tissues. Possible associations between expression and patient clinicopathological characteristics and survival were analyzed. In the NSCLC tissue samples, vascular endothelial growth factor (VEGF) C, VEGFD, VEGFR3, VEGFR2, VEGFR1, lymphatic vessel endothelial hyaluronan receptor 1, integrin subunit α 9, FOX2, neuropilin 2, fibroblast growth factor 2 genes were significantly downregulated (P<0.001 for all) compared with matched normal lung tissues, whereas mRNA levels for VEGFA, spleen associated tyrosine kinase, podoplanin, and prospero homeobox 1 genes were similar in both tissues. Neither lymph node status, nor disease pathological stage influenced expression, whereas more profound suppression of gene activities appeared to occur in squamous cell carcinomas compared with adenocarcinomas. The VEGFR1 mRNA expression level was significantly connected with patient survival in the univariate analysis, and was an independent prognostic factor for overall survival in the multivariate Cox's proportional hazards model (HR 2.103; 95% confidence interval: 1.005-4.401; P=0.049). The results support a hypothesis of absence of new lymphatic vessel formation inside growing NSCLC tumor mass, however do not exclude a possibility of lymphangiogenesis in narrow marginal tumor parts.

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Section: Discussionmentioning

confidence: 98%

Lymphatics-associated genes are downregulated at transcription level in non-small cell lung cancer

Kowalczuk

Laudański

et al. 2018

Oncol Lett

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“…The VEGF family contains many members (Clauss, 2000). Among them, VEGF-A and VEGF-C are abnormally up-regulation in EC, in which they are related to worse prognosis (Juchniewicz et al, 2015; Kimura et al, 2008; Kozłowski et al, 2013). VEGF-A and VEGF-C mRNA were up-regulated in EC, whereas overexpression of VEGF-C in ESCC was more significant in this research.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of VEGF-C and MMP-9 predicts poor prognosis in Kazakh patients with esophageal squamous cell carcinoma

Xie

Wang

et al. 2019

PeerJ

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Vascular endothelial growth factor (VEGF) and Matrix metalloproteinases (MMPs) are believed to participate in infiltration of tumors. High mortality of esophageal squamous cell carcinoma (ESCC) related to its primary infiltration; however, it is not clear whether the expression of VEGF and MMPs is involved in this process. Screening of The Cancer Genome Atlas (TCGA) database showed that among the VEGF family and MMP9, VEGF-A, VEGF-C, and MMP-9 mRNA were overexpression in ESCC. This result was verified using the Oncomine database and in Kazakh patients with ESCC. Overexpression of VEGF-C and MMP-9 and positive association with advanced esophageal cancer and invading ESCC cells (Gene Expression Omnibus (GEO): ). Immunohistochemical staining revealed that VEGF-C and MMP-9 were overexpressed in Kazakh ESCCs. VEGF-C expression was related to invasive depth, tumor-node-metastasis (TNM) staging, lymphatic, and lymph node metastasis of ESCC. The linear association between them was further confirmed in TCGA database and the specimens from Kazakh patients with ESCC. Patients with both proteins expression had tumors with greater aggressiveness, suffered from poor prognosis compared with patients who did not express either protein or expressed protein alone. Both proteins expression predicted high invasiveness of ESCC, which is related to worse prognosis of Kazakh ESCCs.

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“…Many recent studies have revealed a strong association between VEGF-C expression and clinicopathological data, i.e. grade of histological malignancy, progression, dissemination as well as prognosis of the disease (12)(13)(14)(15). In breast, prostate and colon cancer increased expression of VEGF-C correlated with high lymphatic vessel density (LVD) and poor survival (16)(17)(18)(19)(20)(21).…”

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confidence: 99%

Podoplanin Expression Correlates with Disease Progression in Mycosis Fungoides

Jankowska‐Konsur

Kobierzycki²,

Grzegrzółka³

et al. 2017

Acta Derm Venerol

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The aim of this study was to investigate the role of lymphangiogenesis in the clinical progression and outcome of mycosis fungoides. Immunohistochemistry and Western blot techniques were used to assess the expression of podoplanin and vascular endothelial growth factor C in mycosis fungoides. Expression of vascular endothelial growth factor C measured by immunohistochemistry was significantly higher in mycosis fungoides samples in comparison with control cases (chronic benign dermatoses) (p = 0.0012). Increased expression of podoplanin was found in advanced vs. early mycosis fungoides (p < 0.0001), and was positively correlated with cutaneous and nodal involvement (p < 0.001, p < 0.0001; respectively). Higher podoplanin expression was also significantly associated with shorter survival (p < 0.001). Strong positive correlation was observed between expression of podoplanin analysed by immunohistochemistry and Western blot (r = 0.75, p < 0.0001). A similar association was shown regarding expression of vascular endothelial growth factor C (r = 0.68, p = 0.0007). In conclusion, these results suggest that increased expression of podoplanin is associated with poor clinical course, as well as shorter survival, of patients with mycosis fungoides.

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Prognostic value of vascular endothelial growth factor-C and podoplanin mRNA expression in esophageal cancer

Cited by 9 publications

References 23 publications

Lymphatics-associated genes are downregulated at transcription level in non-small cell lung cancer

Lymphatics-associated genes are downregulated at transcription level in non-small cell lung cancer

Overexpression of VEGF-C and MMP-9 predicts poor prognosis in Kazakh patients with esophageal squamous cell carcinoma

Podoplanin Expression Correlates with Disease Progression in Mycosis Fungoides

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