Prognostic value of post thaw semen quality parameters, mitochondrial integrity and cholesterol content of sperm membrane vis-à-vis conception rate in Frieswal bulls

Srivastava, N.; Pande, Megha; Raja, T. V.; Tyagi, S.; Kumar, Suresh; Kumar, Sushil; Kumar, Ravinder; Sirohi, A. S.; Chand, Naimi; Arya, Sargam; Kumar, Amit; Din, Omer

doi:10.56093/ijans.v88i8.82911

Cited by 2 publications

(1 citation statement)

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“…The assessment of mitochondrial membrane potential is a useful test for predicting sperm fertilization capacity in males [70]. In Frieswal bulls, MMP was found to be strongly correlated with bull fertility [71]. In the present study, HNG supplementation resulted in significantly improved MMP in all the TGs as compared to the control.…”

Section: Discussionsupporting

confidence: 54%

Novel Antioxidant Humanin Analogue Restores Bull Semen Cryosurvivability

Pande,

Ghosh,

Tyagi

et al. 2024

Andrologia

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The current study was intended to determine the effects of humanin analogue (HNG) supplementation on semen freezability and oxidative stress parameters of cryopreserved bull spermatozoa. A total of 24 semen ejaculates, from Frieswal breeding bulls, were selected for the experiment. Samples with ≥70% progressive motility, ≥500 million spermatozoa/mL, and >2 mL volume were utilized for further processing, supplementation, and cryopreservation. Each sample was divided into four equal groups and the experiment consisted of three treatment groups and one control group. In the control group, the spermatozoa were frozen in an extender without HNG, while in the other three groups, HNG supplementation was done at a concentration of 2, 10, and 20 μM, respectively. The fortification of HNG at a concentration of 10 and 20 µM significantly (p <0.05) improved the percent post-thaw motility and kinetic variables as assessed by CASA, viability, acrosome- and plasma membrane-integrity as compared to 2 µM and control group. The supplementation showed significant (p <0.05) controlled oxidative stress as exhibited by a reduction in lipid peroxidation and reactive oxygen species levels. The supplemented groups also exhibited significant (p <0.05) improvements in total antioxidant levels, increased mean percentage of spermatozoa exhibiting high mitochondrial membrane potential, DNA integrity, acrosome-intact noncapacitated spermatozoa, and zona binding ability. In conclusion, the supplementation of humanin analogue at 10 and 20 µM significantly improved the post-thaw semen quality, functional parameters, and zona binding ability and controlled the oxidative stress in cryopreserved spermatozoa. Thus, the supplementation of its analogue may potentially prove to be a valuable approach for the improvement of semen freezability in crossbred Frieswal bulls.

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Section: Discussionsupporting

confidence: 54%

Novel Antioxidant Humanin Analogue Restores Bull Semen Cryosurvivability

Pande,

Ghosh,

Tyagi

et al. 2024

Andrologia

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Effects of Unconjugated Gold, Silver and Titanium Dioxide Nanoparticles on Bovine Spermatozoa at Various Stages of Cryopreservation

et al. 2022

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BACKGROUND:The increasing use of nanoparticles (NP) for gender-selected spermatozoa, spermenriched semen and novel extenders raises the concern of undesirable effects on fertility and sperm function.OBJECTIVE:To investigate the effects of gold (Au-), silver (Ag-), and titanium dioxide (TiO2-) NPs on the motility and sperm functions in bovine spermatozoa at various stages of cryopreservation.MATERIALS AND METHODS:Frieswal (Sahiwal × Holstein Friesian) bull semen ejaculates (N = 24) were challenged with unconjugated and ligand-free Au-, Ag-, and TiO2-NPs.RESULTS:At post-dilution (fresh) stage, there was no significant difference observed in progressive motility and viability amongst the control and any nanoparticle-treated groups, though plasma membrane integrity was significantly reduced in nanoparticle-treated groups (p < 0.05). The acrosome intactness was also significantly reduced in the groups of Ag-NP and TiO2-NP (p < 0.05), while there was no effect observed in the Au-NP group. At post-equilibration stage, a significant reduction in motility, viability, and plasma membrane integrity was observed in all three nanoparticle-treated groups (p < 0.05). There was no difference in intact acrosome between the control and Au-NPs groups; which was significantly higher than the Ag-NP and TiO2-NP groups (p < 0.05). At post-thaw stage, all NP groups resulted in a significant reduction of motility, viability, acrosome intactness and plasma membrane integrity (p< 0.05). Besides, TiO2-NPs appear to be significant more toxic (p< 0.05) among three NP groups, and Au-NPs appear to be lesser toxic.CONCLUSION:Bovine spermatozoa are adversely affected by Au-, Ag- and TiO2-NPs that may impair sperm motility and other functions.

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Prognostic value of post thaw semen quality parameters, mitochondrial integrity and cholesterol content of sperm membrane vis-à-vis conception rate in Frieswal bulls

Cited by 2 publications

References 22 publications

Novel Antioxidant Humanin Analogue Restores Bull Semen Cryosurvivability

Novel Antioxidant Humanin Analogue Restores Bull Semen Cryosurvivability

Effects of Unconjugated Gold, Silver and Titanium Dioxide Nanoparticles on Bovine Spermatozoa at Various Stages of Cryopreservation

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