Prognostic Value of Phosphorylated Akt and Survivin Expression in Gastric Adenocarcinoma

Abstract

Background : pAkt (the phosphorylated form of the proto-oncogene protein c-akt) and survivin (human BIRC5 protein) are candidate apoptosis-related molecules that may be responsible for cancer progression. The aim of this study was to determine the expression of pAkt and survivin in malignant stomach neoplasm, and their value as prognostic indicators of cancer. Methods : The expression of pAkt and survivin in 144 cases of gastric cancer was detected by immunohistochemistry and compared with established clinicopathological parameters and prognosis of this disease. Results : Expression of pAkt showed significant correlations with depth of invasion, lymph node and distant metastasis, as well as the stage (p < 0.05 for all three correlations), but not with the Lauren classification. Survivin expression closely correlated with histological type, Lauren classification, depth of invasion, metastasis, and stage (p < 0.05 for all). The overall survival of patients with pAkt/survivin expression was inferior to that of patients with loss of pAkt/survivin expression. Cox multivariate analysis demonstrated a significant correlation between stage (p = 0.04), survivin expression (p = 0.02), and prognosis. Conclusions : Patients with pAkt/survivin expression in gastric cancer are at increased risk of cancer-related mortality via the apoptosis resistance pathway. Expression of pAkt and survivin could be used as a prognostic indicator for gastric cancer.

Transcriptome analysis reveals GPNMB as a potential therapeutic target for gastric cancer

“…Therefore, there is an urgent need for a better understanding of the detailed mechanism to develop novel strategies to diagnose and treat gastric cancer. that the PI3K/AKT signaling pathway is a key pathway involved in gastric carcinogenesis, which was in line with previous studies (Kobayashi, Semba, Matsuda, Kuroda, & Yokozaki, 2006;Lee et al, 2010;Osaki et al, 2004).…”

“…Pathway analysis showed that many DEGs are related to canonical oncogenic pathways. Both pathways and GSEA enrichment analyses indicated that the PI3K/AKT signaling pathway is a key pathway involved in gastric carcinogenesis, which was in line with previous studies (Kobayashi, Semba, Matsuda, Kuroda, & Yokozaki, 2006;Lee et al, 2010;Osaki et al, 2004).…”

Clinicopathological correlations of mTOR and pAkt expression in non-small cell lung cancer

“…Diverse opinions also exist regarding prognosis in relation to pAkt expressions. A large majority of studies have asserted that pAkt expression is correlated with poor prognosis in NSCLC [11,17,26], breast cancer [27], gastric cancer [20], and renal cell carcinoma [15]. However, Chan et al [28] have reported that pAkt expression is not significantly related to patient survival, but is inversely related to tumor grade and size in breast cancer.…”

“…Once activated, signaling through Akt can be propagated to a diverse array of substrates, including mTOR, a key regulator of protein translation [9]. Moreover, activation of the Akt/mTOR pathway confers resistance to many types of cancer therapy [10][11][12][13][14] and is a poor prognostic factor for many cancers [15][16][17][18][19][20]. However, the prognostic significance and predictive value for response to therapy targeting activated Akt (phosphorylated Akt) or mTOR is not clearly established in the lung cancer patients.…”