Prognostic Value of Multiple Reverse Transcription-PCR Tyrosinase Testing for Circulating Neoplastic Cells in Malignant Melanoma

Szenajch, Jolanta; Jasiński, B; Synowiec, Agnieszka; Kulik, Jadwiga; Chomicka, M; Strużyna, Jerzy; Nowecki, Zbigniew; Rutkowski, Piotr; Ruka, W.; Kupść, W; Siedlecki, Janusz A.; Wiktor-Jedrzejczak, W

doi:10.1373/49.9.1450

Cited by 33 publications

(25 citation statements)

References 37 publications

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“…More importantly, detection of CMCs was shown to be an indicator of early relapse and poor survival from melanoma [64,[66][67][68][70][71][72]74]. Multiple tests were shown to increase the sensitivity of detection of CMCs [ [75][76][77] and tests carried out up to 6 months after surgical removal of localized melanoma were still shown to be associated with an adverse prognosis [70]. Curry et al [75] found that tests on three blood samples taken in the first 3 months after surgical removal of melanoma provided the highest sensitivity for detection of CMCs.…”

Section: Staging Using Markers In the Blood Circulating Melanoma Cellsmentioning

confidence: 99%

The history and future of melanoma staging

Thompson

Shaw

Hersey

et al. 2004

Journal of Surgical Oncology

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The evolution and progressive refinement of an internationally accepted melanoma staging system over the last 50 years has resulted in much greater accuracy and increased utility, but the staging process has become more complex and less intuitive. This raises the question of whether melanoma staging should continue to develop with ever-increasing levels of complexity, or whether attempts should be made to produce an alternative system that is simpler and more intuitive. The current, TNM-based American Joint Committee on Cancer (AJCC) staging system for melanoma incorporates only some of the prognostic factors of proven significance. However, the information that is now available about these and other, well-documented prognostic factors allows accurate prediction of an individual melanoma patient's prognosis using a computer-generated estimate. Thus an alternative staging strategy that could be considered in the future would be to use such an estimate to obtain a numerical score for each patient, based on all available information agreed to be of prognostic relevance. A stage grouping could then be assigned on the basis of that score, according to previously determined score ranges for each stage and substage. The advantages of such a system would be that it would allow more reliable comparison of treatment results within and between institutions, and would provide more equivalent stratification groups for patients entering clinical trials of new therapies and those entering adjuvant therapy trials. A further advantage would be that because there would be a direct link between staging and prognostic estimate, such a system would be more readily able to be understood in an intuitive fashion.

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Section: Staging Using Markers In the Blood Circulating Melanoma Cellsmentioning

confidence: 99%

The history and future of melanoma staging

Thompson

Shaw

Hersey

et al. 2004

Journal of Surgical Oncology

View full text Add to dashboard Cite

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“…Previously, Wong et al (11 ) have demonstrated that systemic chemotherapy could apparently remove the CTCs in blood of liver cancer patients. Although systemic chemotherapy, immunotherapy, and chemoimmunotherapy could possibly eradicate the CMCs, Szenajch et al (8 ) did not notice any critical effects of systemic treatment on the persistence of CMCs in melanoma patients. Apparently, multiple TYR mRNA diagnostic testing can be useful for molecular cancer staging for melanoma patients and hence for prediction of relapse or development of metastases.…”

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confidence: 96%

“…The frequency of TYR mRNA positivity during repeated testing was significantly associated with melanoma stage, recurrence, and overall survival (8 ). Although a single positive TYR test could indicate the risk of relapse, multiple RNA diagnostic testing on multiple blood samples may predict cancer progression more accurately.…”

mentioning

confidence: 99%

“…The frequency of positivity after multiple TYR testing appeared to increase significantly with melanoma stage, independent of the time interval between consecutive TYR RT-PCR tests. The majority of melanoma patients with visceral metastases were systemically treated with chemotherapy, immunotherapy, and chemoimmunotherapy (8 ). Previously, Wong et al (11 ) have demonstrated that systemic chemotherapy could apparently remove the CTCs in blood of liver cancer patients.…”

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confidence: 99%

“…Although multiple nested RT-PCR testing can enhance the sensitivity and specificity for detecting CTCs, this technique has posed a major problem of carryover contamination with the first-step PCR products (8,17 ). The sporadic detection of PSA mRNA may possibly reflect its low basal concentration in blood, leading to false negativity or stochastic effects of multiple RT-PCR testing in relation to sampling or pipetting errors.…”

mentioning

confidence: 99%

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