Prognostic value of microRNA-21 in pancreatic ductal adenocarcinoma: a meta-analysis

Hu, Gengyuan; Feng, Tao; Wang, Wei; Ji, Kewei

doi:10.1186/s12957-016-0842-4

Cited by 25 publications

(30 citation statements)

References 53 publications

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“…There is growing evidence that miRNA expression profiles have a potential to provide tumor-specific prognostic information. Several recent works have reported associations between microRNA expression and overall survival in PDAC patients [22,57,63,64]. In our study we observed no correlation between expression of all selected miRNAs and OS or PFS (Tables 8 and 9).…”

Section: Correlation Of Clinicopathological Features Of Tumors With Mcontrasting

confidence: 48%

“…Abnormal expression of miR-21 as a marker of poor patient outcome has also been demonstrated in many other malignancies, including melanoma, glioma, carcinomas of breast, colorectal, lung, stomach, prostate, hepatocellular carcinoma and renal cell carcinoma [65]. In patients with PDAC, several meta-analyses, assessing more than 20 articles, published from 2007 to 2016, including more than 2000 PDAC cases [22,[63][64][65][66] Giovanetti et al detected, however, significantly higher mir-21 levels in in microdissected tumor cells, correlating with significantly shorter OS [57] The role of miR-96 expression levels in patient prognosis has not been analyzed extensively. Li et al showed that downregulated expression of miR-96-5p was associated with a decreased overall survival in patients with PDAC [72].…”

Section: Correlation Of Clinicopathological Features Of Tumors With Mmentioning

confidence: 99%

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Expression of selected MicroRNAs in pancreatic ductal adenocarcinoma in relation to tumor progression and patient’s outcome.

Popov

Szabó²,

Gürlich³

et al. 2019

Preprint

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Background: pancreatic ductal adenocarcinoma (PDAC) remains a disease with extremely poor prognosis and limited effective available treatment. Differential expression of miRNAs isolated from tumor tissue has been proposed as a marker for tumor diagnosis, progression and prognosis. Nevertheless, prognostic value of miRNAs expression in PDACs for patient outcome still remains unclear.Methods: expression of 7 selected miRNAs, isolated from FFPE samples of 54 PDAC patients, was quantified using RT-qPCR. The relationship of miRNA expression levels with tumor histology, clinico-pathological characteristics, patient overall survival (OS) and progress-free survival (PFS), was subsequently evaluated.Results: overexpression of miR-21, miR-155 and miR-210 was observed in PDACs (up to 72.62-fold, 232.36 and 181.38-fold respectively), in comparison with non-neoplastic tissues. On the other hand, miR-96 and miR-217 were significantly downregulated in PDACs (up to one hundred times). No differences were, however, noticed between cancer and normal tissues for the expression levels of miR-148a and miR-196a.On the other hand, expression levels of all 7 miRNAs failed to demonstrate significant correlation with parameters of tumor progression, such as tumor stage, grade, nodal involvement, perineural and vascular invasion. Positive correlation of miR-210 levels was, however, observed with patient age (ρ=0.35). Additionally, miR-148a and miR-217 expression have shown positive association with tubular tumor growth pattern (ρ=0.39; ρ=0.28). Negative correlation of miR-148a values was also demonstrated with dissociative growth pattern and nuclear atypia (ρ=-0.30; ρ=0.27).

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Section: Correlation Of Clinicopathological Features Of Tumors With Mcontrasting

confidence: 48%

Section: Correlation Of Clinicopathological Features Of Tumors With Mmentioning

confidence: 99%

Expression of selected MicroRNAs in pancreatic ductal adenocarcinoma in relation to tumor progression and patient’s outcome.

Popov

Szabó²,

Gürlich³

et al. 2019

Preprint

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“…Hu et al conducted a systematic review of twelve publications discussing the prognostic role of miR-21. They conclude that elevated miR-21 expression levels significantly predict for worse OS in pancreatic cancer [ 17 ]. Khan et al prospectively collected plasma samples from twelve patients with locally advanced unresectable pancreatic cancer.…”

Section: Methodsmentioning

confidence: 99%

Prognostic value of microRNA expression levels in pancreatic adenocarcinoma: a review of the literature

et al. 2017

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BackgroundClinical and pathologic markers of prognosis and patterns of failure help guide clinicians in selecting patients for adjuvant therapy after surgical resection for pancreatic adenocarcinoma (PDAC). Recent studies have reported the prognostic utility of microRNA profiling in numerous malignancies. Here, we review and summarize the current literature regarding associations between microRNA expression and overall survival in PDAC patients.Materials and MethodsWe conducted a systematic search in the PubMed database to identify all primary research studies reporting prognostic associations between tumor and/or serum microRNA expression and overall survival in PDAC patients. Eligible articles were reviewed by the authors and relevant findings are summarized below.ResultsWe found 53 publications that fit our search criteria. In total, 23 up-regulated and 49 down-regulated miRNAs have been associated with worse overall survival. MiR-21 is the most commonly reported miRNA, appearing in 19 publications, all of which report aberrant over-expression and association with shorter survival in PDAC. Other miRNAs that appear in multiple publications include miR-10b, −21, −34a, −155, −196a, −198, −200c, −203, −210, −218, −222, and −328. We summarize the preclinical and clinical data implicating these miRNAs in various molecular signaling pathways and cellular functions.ConclusionsThere is growing evidence that miRNA expression profiles have the potential to provide tumor-specific prognostic information to assist clinicians in more appropriately selecting patients for adjuvant therapy. These molecules are often aberrantly expressed and exhibit oncogenic and/or tumor suppressor functions in PDAC. Additional efforts to develop prognostic and predictive molecular signatures, and further elucidate miRNA mechanisms of action, are warranted.

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“…miR-21 is overexpressed in various types of tumors. The following are examples of confirmed roles of the miR-21 in tumors: breast [ 40 ], lung [ 41 ], ovarian [ 42 ], cervical [ 43 , 44 ], colon [ 38 ], brain [ 45 , 46 ], liver [ 47 , 48 ], prostate [ 49 ], and pancreatic cancer [ 50 , 51 ]. Dysregulation of miR-21 makes it a very good candidate as a biomarker for developing tools for the early diagnosis and prognosis of cancers.…”

Section: Introductionmentioning

confidence: 99%

Micro RNA Sensing with Green Emitting Silver Nanoclusters

Yourston

Krasnoslobodtsev

2020

Molecules

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Micro RNA (miR) are regulatory non-coding RNA molecules, which contain a small number of nucleotides ~18–28 nt. There are many various miR sequences found in plants and animals that perform important functions in developmental, metabolic, and disease processes. miRs can bind to complementary sequences within mRNA molecules thus silencing mRNA. Other functions include cardiovascular and neural development, stem cell differentiation, apoptosis, and tumors. In tumors, some miRs can function as oncogenes, others as tumor suppressors. Levels of certain miR molecules reflect cellular events, both normal and pathological. Therefore, miR molecules can be used as biomarkers for disease diagnosis and prognosis. One of these promising molecules is miR-21, which can serve as a biomarker with high potential for early diagnosis of various types of cancer. Here, we present a novel design of miR detection and demonstrate its efficacy on miR-21. The design employs emissive properties of DNA-silver nanoclusters (DNA/AgNC). The detection probe is designed as a hairpin DNA structure with one side of the stem complimentary to miR molecule. The binding of target miR-21 opens the hairpin structure, dramatically modulating emissive properties of AgNC hosted by the C12 loop of the hairpin. “Red” fluorescence of the DNA/AgNC probe is diminished in the presence of the target miR. At the same time, “green” fluorescence is activated and its intensity increases several-fold. The increase in intensity of “green” fluorescence is strong enough to detect the presence of miR-21. The intensity change follows the concentration dependence of the target miR present in a sample, which provides the basis of developing a new, simple probe for miR detection. The detection strategy is specific, as demonstrated using the response of the DNA/AgNC probe towards the scrambled miR-21 sequence and miR-25 molecule. Additionally, the design reported here is very sensitive with an estimated detection limit at ~1 picomole of miR-21.

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Prognostic value of microRNA-21 in pancreatic ductal adenocarcinoma: a meta-analysis

Cited by 25 publications

References 53 publications

Expression of selected MicroRNAs in pancreatic ductal adenocarcinoma in relation to tumor progression and patient’s outcome.

Expression of selected MicroRNAs in pancreatic ductal adenocarcinoma in relation to tumor progression and patient’s outcome.

Prognostic value of microRNA expression levels in pancreatic adenocarcinoma: a review of the literature

Micro RNA Sensing with Green Emitting Silver Nanoclusters

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