Prognostic value of matrix metalloproteinases (MMP-2 and MMP-9) in Hodgkin's and non-Hodgkin's lymphoma

Abstract

Matrix metalloproteinases (MMPs) are responsible for the degradation of extracellular matrix and have an important role in tumour metastases. We investigated the role of MMP-2 and MMP-9 in Hodgkin's disease (HD) and non-Hodgkin's lymphoma (NHL). The serum samples of patients with HD (n = 12), NHL (n = 30) and healthy control (n = 22) were analysed for MMP-2 and MMP-9. An immunoassay method was used for the determination of MMP-2 and MMP-9 levels. No statistical significance was found between HD and NHL groups for levels of MMP-2. There were no relation between MMP-2, MMP-9 levels and clinical characteristics of patients. The mean MMP-9 levels were found to be 555.6 +/- 140 ng/ml, 446.6 +/- 53.6 ng/ml and 111.2 +/- 10.3 ng/ml in HD, NHL and control groups, respectively. Our results suggest that MMP-9 levels are substantially increased in HD and NHL when compared with controls and may probably be used for distinguishing the benign diseases from malign lymphomas.

Involvement of the Syk–mTOR pathway in follicular lymphoma cell invasion and angiogenesis

“…The similarity of the results obtained in our study with Syk siRNA knockdown experiments supports the notion that Syk is the main kinase involved in the regulation of MMP-9 expression in our FL cellular models. Several studies have reported elevated MMP-9 expression in NHLs 26,27,32 and, in some studies, a correlation with poor prognosis. 27,38 In our study, we found that MMP-9 expression in vivo was found to be Syk dependant, and that FL patients displayed a higher expression of MMP-9 than healthy donors.…”

“…26, 27 We therefore sought to determine whether this was also the case in FL samples by quantifying MMP-9 serum levels using enzymelinked immunosorbent assay. We observed that sera from FL patients displayed a 3.3-fold increase in secreted MMP-9 levels when compared with healthy donors (789.7±193.2 vs 240.8 ± 52.2 ng/ml, Po0.005; Figure 4a).…”

“…This is consistent with results obtained by other groups. 26,33 We also analyzed MMP-9 expression by immunohistochemistry on biopsy sections isolated from FL-affected lymph nodes. As shown in Figure 4b, cells derived from FL patients displayed an intense MMP-9 staining in the cytosol.…”

“…18 The expression levels of VEGF mRNA and protein also correlate with malignant progression and poor prognosis in nonhematopoietic malignancies. 24,25 In non-Hodgkin's lymphomas (NHLs), a high expression of MMP-9 has been correlated with tumor grade 26,27 and high VEGF serum levels have been linked to poor clinical outcome. 28 However, NHLs correspond to a heterogeneous group of malignancies with variable clinical course and these studies did not focus on FL.…”

Plasmin inhibitor reduces T-cell lymphoid tumor growth by suppressing matrix metalloproteinase-9-dependent CD11b+/F4/80+ myeloid cell recruitment

“…Increased plasma MMP-9 levels were reported in Hodgkin's and non-Hodgkin's lymphoma patients. 5 But the distinct role of MMP-9 and the relation between Plg and MMP-9 in lymphoma growth is still not clear.…”