Prognostic Value of Cyclin B1 Protein Expression in Colorectal Cancer

Grabsch, Heike; Lickvers, Kristina; Hansen, Olaf; Takeno, Shinsuke; Willers, Reinhart; Stock, Wolfgang G.; Gabbert, Helmut E.; Mueller, Wolfram

doi:10.1309/54h4q88a1ubbwpte

Cited by 26 publications

(12 citation statements)

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“…Several reports show that cyclin B1 levels are elevated in cancer cells (Dutta et al, 1995;Mashal et al, 1996;Kushner et al, 1999;Yasuda et al, 2002;Grabsch et al, 2004). Overexpression of cyclin B1 is associated with a poor prognosis for patients with squamous cell carcinomas of the esophagus (Murakami et al, 1999;Takeno et al, 2002), larynx (Dong et al, 2002), lung (Soria et al, 2000) and tongue (Hassan et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

A decrease in cyclin B1 levels leads to polyploidization in DNA damage‐induced senescence

Kikuchi

Nakayama

Morinaga

et al. 2010

Cell Biology International

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Adriamycin, an anthracycline antibiotic, has been used for the treatment of various types of tumours. Adriamycin induces at least two distinct types of growth repression, such as senescence and apoptosis, in a concentration-dependent manner. Cellular senescence is a condition in which cells are unable to proliferate further, and senescent cells frequently show polyploidy. Although abrogation of cell division is thought to correlate with polyploidization, the mechanisms underlying induction of polyploidization in senescent cells are largely unclear. We wished, therefore, to explore the role of cyclin B1 level in polyploidization of Adriamycin-induced senescent cells. A subcytotoxic concentration of Adriamycin induced polyploid cells having the features of senescence, such as flattened and enlarged cell shape and activated beta-galactosidase activity. In DNA damage-induced senescent cells, the levels of cyclin B1 were transiently increased and subsequently decreased. The decrease in cyclin B1 levels occurred in G2 cells during polyploidization upon treatment with a subcytotoxic concentration of Adriamycin. In contrast, neither polyploidy nor a decrease in cyclin B1 levels was induced by treatment with a cytotoxic concentration of Adriamycin. These results suggest that a decrease in cyclin B1 levels is induced by DNA damage, resulting in polyploidization in DNA damage-induced senescence.

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Section: Discussionmentioning

confidence: 99%

A decrease in cyclin B1 levels leads to polyploidization in DNA damage‐induced senescence

Kikuchi

Nakayama

Morinaga

et al. 2010

Cell Biology International

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“…It is consistent with clinical application of large dose progesterone in treatment for endometrial proliferative disorders. As abundant expression of Cyclin B1 and alteration of the G2 pathway was reported in abnormal proliferative endometrial tissues, such as endometrial hyperplasia, endometrial adenocarcinoma and endometriosis[16,18,19,22-24] the down-regulation of cyclin B1 detected under the treatment of progesterone could well explain the underlying mechanism of the inhibitory effect of progesterone on endometrial cell growth.…”

Section: Discussionmentioning

confidence: 99%

“…Cyclin B1 binds to Cdc2 at the beginning of G2 phase forming an activated cyclin B1/Cdc2 complex and then phosphorylates its downstream substrates which control the G2 to M transition and promote cell mitotic division[15]. Unscheduled mis-regulation of cyclin B1 during the cell cycle leads to uncontrolled cell growth and aberrant cell function[16]. It is also reported that cyclins are functionally involved in the rhythmic proliferation of normal human endometrial tissue[17].…”

Section: Introductionmentioning

confidence: 99%

Involvement of cyclin B1 in progesterone-mediated cell growth inhibition, G2/M cell cycle arrest, and apoptosis in human endometrial cell

Tang

Pan

Luo

et al. 2009

Reprod Biol Endocrinol

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BackgroundProgesterone plays an important role in the proliferation and differentiation of human endometrial cells (hECs). Large-dose treatment with progesterone has been used for treatment of endometrial proliferative disorders. However, the mechanisms behind remain unknown.MethodsTo investigate the role of cyclin B1 in proliferation and differentiation of hECs in menstrual cycle, the expression of cyclin B1 throughout the menstrual cycle was evaluated in hECs. To determine the effects of progesterone on the proliferation, cell cycle progression and apoptosis of hECs and to test if cyclin B1 is involved in these effects, progesterone and/or Alsterpaullone (Alp, a specific inhibitor of Cyclin B1/Cdc2) were added to primary hECs. Cellular proliferation was evaluated with MTT test, cell cycle with propidium iodide (PI) staining and flow cytometry, apoptosis with FITC-Annexin V and the expression of cyclin B1 with Western blotting.ResultsThe expression level of cyclin B1 in secretory endometria was significantly lower than in proliferative endometria (p < 0.01). Progesterone significantly inhibited the growth of hECs in a concentration-dependent manner (P < 0.01). The treatment with progesterone significantly decreased the expression of cyclin B1, increased the proportions of cell in G2/M phase, and apoptotic cells (P < 0.05 for all). The presence of Alp significantly enhanced the effects of progesterone on cyclin B1 down-regulation, G2/M cell cycle arrest and induction of apoptosis (P < 0.01 for all).ConclusionOur findings suggest that cyclin B1 is a critical factor in proliferation and differentiation of hECs. Progesterone may inhibit cell proliferation, mediate G2/M cell cycle arrest and induce apoptosis in hECs via down-regulating Cyclin B1.

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“…Many studies even disregard the cytoplasmic staining of cyclins, as it is considered nonspecific [59,63]. Interestingly, in some tumors including NSCLC, cyclin B1 is predominantly observed in the cytoplasm [65,79,80]. Cyclin B1/CDK complexes are thought to remain in the cytoplasm due to DNA damage [81].…”

Section: Cyclins In Nsclcmentioning

confidence: 99%

Prognostic and Predictive Value of Cell Cycle Deregulation in Non-Small-Cell Lung Cancer

2012

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Non-small-cell lung cancer (NSCLC) is among the most frequently diagnosed malignancy and a leading cause for cancer mortality worldwide. Despite various efforts, practical prognostic and predictive markers are still few. We review recent findings concerning the cell cycle in NSCLC and discuss prognostic and predictive aspects as well as the challenge of targeted therapeutic approaches. Deregulation of the cell cycle is a common event in NSCLC. Usually, several defects of cell cycle regulation are concomitant and have a cumulative adverse effect on prognosis. Therefore, analysis of a variety of interacting molecules is desirable for adequate deductions. Immunohistochemical interpretations should include the subcellular staining localization, since this can reflect the functional properties of a protein. Overexpression of cyclins, especially D-type cyclins, has repeatedly been associated with poor prognosis in NSCLC. Predictive data is less conclusive; however, loss of the expression of cyclin-dependent kinase inhibitors seems to correlate with sensitivity to antiproliferative drugs. Various inhibitors of Aurora kinases are currently being evaluated regarding their potential as targeted therapies in NSCLC. In conclusion, the cell cycle offers several prognostic, predictive and therapeutic possibilities in NSCLC, many still developmental. Progress in this field has the potential to improve the current scenario for NSCLC patients.

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Prognostic Value of Cyclin B1 Protein Expression in Colorectal Cancer

Cited by 26 publications

References 0 publications

A decrease in cyclin B1 levels leads to polyploidization in DNA damage‐induced senescence

A decrease in cyclin B1 levels leads to polyploidization in DNA damage‐induced senescence

Involvement of cyclin B1 in progesterone-mediated cell growth inhibition, G2/M cell cycle arrest, and apoptosis in human endometrial cell

Prognostic and Predictive Value of Cell Cycle Deregulation in Non-Small-Cell Lung Cancer

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