Prognostic value of chromosome 1 and 8 copy number in invasive ductal breast carcinoma among iranian women: An interphase FISH analysis

Abstract

Breast cancer is amongst the leading causes of death in women worldwide and the most common cancer amongst Iranian women. Unfortunately, the current clinical and histological criteria can only help 60 percent of women with breast cancer in diagnosis and long-term treatment. Therefore, genetic markers both at single gene and chromosomal level can play an important role in improving the diagnosis and prognosis of breast cancer patients. The aim of this retrospective study was to investigate the role of chromosome 1 and 8 copy number assessed by interphase fluorescence in situ hybridization (FISH), as prognostic parameters in 50 Iranian women, aged 35 to 64 years, with sporadic invasive ductal breast carcinoma. Chromosome 1 and 8 copy numbers were evaluated in relation to established clinicopathological parameters, the immunohistochemical markers ER, PR, P53 and cathepsin D, DNA index by flow cytometry, age and survival status of the patients. FISH using centromeric probes for chromosomes 1 and 8 was applied to interphase cell suspensions prepared from archived, Carnoyfixed tumor cells and selected paraffin-embedded tumor sections. Aneusomy for chromosomes 1 and 8 was present in all 50 patients to different levels. The total abnormality rate for chromosome 1 was 33.92 percent (4.24 percent monosomy and 29.68 percent polysomy), whereas for chromosome 8 this rate was 28.30 percent (6.48 percent monosomy and 21.82 percent polysomy). Statistically significant association (p<0.05) was demonstrated between monosomy 1 and patients' age below 50 years, and between monosomy 1 and poor survival, respectively. Disomy 8 was significantly associated with P53 expression. A borderline significant correlation was demonstrated between polysomy 8 and diploid DNA content, as well as between disomy 1 and disease-free status of the patients. Chromosome 1 and 8 copy numbers may be considered as useful prognostic markers in invasive ductal carcinoma of the breast.

Spontaneously Occurring micronuclei in infiltrating ductal carcinoma of breast: A potential biomarker for aggressive phenotype detection?

“…A limited number of reports have studied a correlation between chromosomal abnormalities and cytological grades of breast cancer 9. The MN score was also correlated with the cytological grades for the first time in the study.…”

“…Several studies have indicated that the screening for CIN may be useful for estimating the biological behavior of cancer patients 7, 8. Although, CIN can be determined by fluorescence in situ hybridization (FISH) based techniques, the availability, and cost of probes limits their use 9. Therefore there is a need to search for the simpler and cheaper methods to assess the presence of CIN in cancer.…”

Low Expression of the bcl2 Gene in Gastric Adenocarcinomas in Mazandaran Province of Iran

“…the cDNA synthesis kit(Fermentas Germany,) has been used. Evaluation of concentration and purity of cDNA was performed using Pico Drop 2000 (Thermo Fischer Scientific Inc) (Behjati et al, 2005;Mowla et al, 2005;Mistry et al, 2008).…”

Predictors of mammography based on Health Belief Model in Khorramabad women

“…Unfortunately, 70% of Iranian women are diagnosed in advanced stages of cancer when it is late for proper treatment. [23] The low survival rate of breast cancer in developing countries is characterized by a lack of awareness and lack of early detection programs, which leads to a high proportion of women presenting with symptoms of end-stage disease and a lack of adequate facilities for diagnosis and treatment. [14] Continuous increase in breast cancer deaths in Iranian women may be due to the low use of screening tests for breast cancer.…”