Prognostic value of CA 125 in transitional cell carcinoma of the bladder

Manvar, Ankur; Wallen, Eric; Pruthi, Raj S.; Nielsen, Matthew E.

doi:10.1586/era.10.186

Cited by 7 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Noteworthy, the only common protein amongst the three cell lines in all conditions was MUC16, whose role in bladder cancer remains still poorly understood (Figure 4A , Supplementary Figures S8 and S9 ; Supplementary Tables S1–S3 ). However, increased MUC16 levels in serum (CA125 test) have been associated with worst prognosis in bladder cancer [ 40 ], suggesting it as part of a malignant phenotype [ 41 ]. In hypoxic conditions, the number of identified glycoproteins was significantly higher in all cell lines (72 in T24;34 in 5637; 16 in HT1376; Figure 4A ) in comparison to normoxia, thus in accordance with the results obtained by flow cytometry and western blot (Figure 3A and 3B ).…”

Section: Resultsmentioning

confidence: 99%

Hypoxia enhances the malignant nature of bladder cancer cells and concomitantly antagonizes proteinO-glycosylation extension

et al. 2016

View full text Add to dashboard Cite

Invasive bladder tumours express the cell-surface Sialyl-Tn (STn) antigen, which stems from a premature stop in protein O-glycosylation. The STn antigen favours invasion, immune escape, and possibly chemotherapy resistance, making it attractive for target therapeutics. However, the events leading to such deregulation in protein glycosylation are mostly unknown. Since hypoxia is a salient feature of advanced stage tumours, we searched into how it influences bladder cancer cells glycophenotype, with emphasis on STn expression. Therefore, three bladder cancer cell lines with distinct genetic and molecular backgrounds (T24, 5637 and HT1376) were submitted to hypoxia. To disclose HIF-1α-mediated events, experiments were also conducted in the presence of Deferoxamine Mesilate (Dfx), an inhibitor of HIF-1α proteasomal degradation. In both conditions all cell lines overexpressed HIF-1α and its transcriptionally-regulated protein CA-IX. This was accompanied by increased lactate biosynthesis, denoting a shift toward anaerobic metabolism. Concomitantly, T24 and 5637 cells acquired a more motile phenotype, consistent with their more mesenchymal characteristics. Moreover, hypoxia promoted STn antigen overexpression in all cell lines and enhanced the migration and invasion of those presenting more mesenchymal characteristics, in an HIF-1α-dependent manner. These effects were reversed by reoxygenation, demonstrating that oxygen affects O-glycan extension. Glycoproteomics studies highlighted that STn was mainly present in integrins and cadherins, suggesting a possible role for this glycan in adhesion, cell motility and invasion. The association between HIF-1α and STn overexpressions and tumour invasion was further confirmed in bladder cancer patient samples. In conclusion, STn overexpression may, in part, result from a HIF-1α mediated cell-survival strategy to adapt to the hypoxic challenge, favouring cell invasion. In addition, targeting STn-expressing glycoproteins may offer potential to treat tumour hypoxic niches harbouring more malignant cells.

show abstract

Section: Resultsmentioning

confidence: 99%

Hypoxia enhances the malignant nature of bladder cancer cells and concomitantly antagonizes proteinO-glycosylation extension

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Painless hematuria is the most common presenting symptom in UC, but painless hematuria is also common in patients with CKD. The sensitivity and speci city of UC protein markers are decreased because the serum protein levels are increased in patients with CKD (9)(10)(11)(12)(13). Intravenous pyelography or urography cannot be performed in patients with CKD because of the exposure of contrast media (14).…”

Section: Introductionmentioning

confidence: 99%

The microRNA prediction models as ancillary diagnosis biomarkers for urothelial carcinoma in patients with chronic kidney disease

Chen

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Urothelial carcinoma is a common urological cancer in patients with chronic kidney disease in Taiwan. However, urinary cystoscopy is an invasive procedure and urine cytology showed low sensitivity for low-grade tumor. High accuracy with noninvasive diagnostic tools for UC is needed for patients with CKD. Methods: A total of 272 urine and 138 plasma samples to detect the miRNA expression levels for establishing UC signatures from patients with CKD. 17 candidate miRNAs of urine and plasma were selected by the PCR array, and a single qRT-PCR assay was used to confirm the results. The website of a smRNA-seq analysis of the clinical specimens was compared to survival status. The miRNA prediction models were produced for predicting UC by multiple logistic regression.Results: Urinary miR-1274a and miR-30a-5p expression levels were significantly lower but miR-19a-5p expression levels were higher in UC when compared with CKD. In plasma samples, miR-155-5p, miR-19b-1-5p, miR-378 and miR-636 showed significantly lower expression in UC compared to those with CKD. The Kaplan-Meier curve showed that lower expression of miR-19a, miR-19b, miR-636 and miR-378, and higher expression of miR-708-5p were associated with poor prognosis in patients with bladder cancer. In addition, we produced classifiers for predicting UC by multiple logistic regression. The urine signature was developed with four miRNAs, and the AUC was 0.8211. Eight miRNA expression levels from both urine and plasma samples were examined, and the AUC was 0.8595.Conclusion: Two miRNA classifiers could improve the drawbacks of current UC biomarker screenings for patients with CKD.

show abstract

“…Painless hematuria is the most common presenting symptom in UC, but painless hematuria is also common in patients with CKD. The sensitivity and specificity of UC protein markers are decreased because the serum protein levels are increased in patients with CKD (9)(10)(11)(12)(13). Intravenous pyelography or urography cannot be performed in patients with CKD because of the exposure of contrast media (14).…”

Section: Introductionmentioning

confidence: 99%

The MicroRNA Prediction Models as Ancillary Diagnosis Biomarkers for Urothelial Carcinoma in Patients With Chronic Kidney Disease

Chen

et al. 2021

Front. Med.

View full text Add to dashboard Cite

Urothelial carcinoma is a common urological cancer in chronic kidney disease patients. Cystoscopy and urine cytology are the clinical diagnostic tools for UC. However, cystoscopy is an invasive procedure, while urine cytology showed low sensitivity for low-grade urothelial tumors. High accuracy with non-invasive tools for UC is needed for CKD patients. Our study collected a total of 272 urine and 138 plasma samples to detect the miRNA expression levels for establishing UC signatures from CKD patients. Seventeen candidate miRNAs of biofluids were selected and confirmed by qRT-PCR. Our results showed that urinary miR-1274a and miR-30a-5p expression levels were significantly lower but miR-19a-5p expression levels were higher in UC when compared with CKD. In plasma samples, miR-155-5p, miR-19b-1-5p, miR-378, and miR-636 showed significantly lower expression in UC compared to those with CKD. The Kaplan-Meier curve showed that lower expression of miR-19a, miR-19b, miR-636 and miR-378, and higher expression of miR-708-5p were associated with poor prognosis in patients with bladder cancer. In addition, we produced classifiers for predicting UC by multiple logistic regression. The urine signature was developed with four miRNAs, and the AUC was 0.8211. Eight miRNA expression levels from both urine and plasma samples were examined, and the AUC was 0.8595. Two miRNA classifiers and the nomograms could improve the drawbacks of current UC biomarker screenings for patients with CKD.

show abstract

Prognostic value of CA 125 in transitional cell carcinoma of the bladder

Cited by 7 publications

References 16 publications

Hypoxia enhances the malignant nature of bladder cancer cells and concomitantly antagonizes proteinO-glycosylation extension

Hypoxia enhances the malignant nature of bladder cancer cells and concomitantly antagonizes proteinO-glycosylation extension

The microRNA prediction models as ancillary diagnosis biomarkers for urothelial carcinoma in patients with chronic kidney disease

The MicroRNA Prediction Models as Ancillary Diagnosis Biomarkers for Urothelial Carcinoma in Patients With Chronic Kidney Disease

Contact Info

Product

Resources

About