Prognostic value of a microRNA signature in nasopharyngeal carcinoma: a microRNA expression analysis

PurposeTo analyse miRNA regulation in a rat model of acute ocular hypertension (AOH).MethodsAcute ocular hypertension (AOH) was induced in the left eye of adult albino rats by inserting a cannula connected with a saline container into the anterior chamber. The contralateral eye served as a control. Seven days later, animals were killed. Retinas were used either for quantitative analysis of retinal ganglion cells (RGCs) and microglial cells or for miRNA array hybridization, qRT‐PCR and Western blotting.ResultsAnatomically, AOH caused axonal degeneration, a significant loss of RGCs and a significant increase in microglial cells in the ganglion cell layer. The miRNAs microarray analysis revealed 31 differentially expressed miRNAs in the AOH versus control group, and the regulation of 12 selected microRNAs was further confirmed by qRT‐PCR. Bioinformatic analysis indicates that several signalling pathways are putatively regulated by the validated miRNAs. Of particular interest was the inflammatory pathway signalled by mitogen‐activated protein kinases (MAPKs). In agreement with the in silico analysis, p38 MAP kinase, tumour necrosis factor‐alpha (TNF‐α) and iNOS proteins were significantly upregulated in the AOH retinas.ConclusionsAcute IOP elevation led to changes in the expression of miRNAs, whose target genes were associated with the regulation of microglia‐mediated neuroinflammation or neural apoptosis. Addressing miRNAs in the process of retinal ischaemia and optic nerve damage in association with high IOP elevation may open new avenues in preventing retinal ganglion cell apoptosis and may serve as target for future therapeutic regimen in acute ocular hypertension and retinal ischaemic conditions.

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“…U6 snRNA was used as endogenous control for the quantification of miRNAs (Liu et al. 2012a). Each sample was measured three times.…”

Section: Methodsmentioning

confidence: 99%

MicroRNA regulation in an animal model of acute ocular hypertension

Wang

Valiente‐Soriano

Nadal-Nicolás

et al. 2016

Acta Ophthalmologica

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“…Another recent study has identified a five-miRNA signature (miR-142-3p, miR-29c, miR-26a, miR-30e and miR-93) significantly associated with disease-free survival in nasopharyngeal carcinoma patients. This biomarker panel may add prognostic value to the TNM staging system and inform treatment decisions for patients at high risk of progression [15].…”

Section: Mirnas As Molecular Predictors Of Response To Anticancer Trementioning

confidence: 99%

Great potential of miRNAs as predictive and prognostic markers for cancer

Cho¹

2012

Expert Review of Molecular Diagnostics

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“…14 Increasing evidences suggest that aberrant expression of miRNAs, such as that of miR-93, 15 miR-143, 16 miR-211, 17 and miR-185, 18 contributes to the pathogenesis of NPC. 19 However, it is still not clear how miRNAs control NPC progression and metastasis. 20 The miR-200 family of miRNAs is comprised of five members.…”

Section: Introductionmentioning

confidence: 99%