Prognostic value and susceptibility of BAX rs4645878 polymorphism in cancer

Feng, Yu; Chen, Xianglei; Zheng, Yi; Li, Qiao; Chen, Haiwen; Cai, Yuanhua; Cao, Lixia; Lai, Xiaolin; Pan, Lili; Li, Yang; Wang, Shaoyuan

doi:10.1097/md.0000000000011591

Cited by 13 publications

(11 citation statements)

References 30 publications

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“…The gene encoding Bax protein is located on chromosome 19q13.3 and is controlled by p53. [32][33][34] The results of our study showed that the expression of the Bax gene significantly increased in A-172 cells (P < 0.01), but no significant change was observed in U251 cells. That may be due to a mutation of the p53 gene in U251 cells.…”

Section: Discussionmentioning

confidence: 99%

The effect of miR‐579 on the PI3K/AKT pathway in human glioblastoma PTEN mutant cell lines

Kalhori

Irani

Soleimani

et al. 2019

J of Cellular Biochemistry

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Glioblastoma multiform (GBM) is a type of aggressive brain cancer with limited success in standard treatment. MicroRNAs are one of the most beneficial tools for diagnosis, prognosis, and treatment of cancer. This study aimed to investigate the effect of miR‐579 on cellular behaviors and expression of PI3K/AKT signaling pathway in GBM cell lines. In the present study, miR‐579 was overexpressed in U251 and A‐172 cell lines by using lentil vector, and its effect on cellular behavior such as proliferation and migration was investigated by the cell cycle, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT), Annexin V, colony formation, Transwell and wound healing assays. MiR‐579 predicted target genes (AKT1, Rheb, PDK1, and a few others) were also evaluated by real‐time polymerase chain reaction or luciferase assay and Western blot analysis. Our results represented that overexpression of miR‐579 could inhibit proliferation, migration, cell cycle and also promoted the apoptosis of GBM cell lines. The luciferase reporter assay showed miR‐579 directly targets the 3 UTR of mTOR, Rheb, and PDK1 and repressed their expressions. Furthermore, the Western blot analysis showed that miR‐579 could downregulate the AKT1 and Rheb protein expression. Overall, our findings propose that miR‐579 functions as a novel tumor suppressor gene in GBM by regulating the PI3K/AKT signaling pathway and may serve as a therapeutic target for clinical therapy of glioblastoma multiform.

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Section: Discussionmentioning

confidence: 99%

The effect of miR‐579 on the PI3K/AKT pathway in human glioblastoma PTEN mutant cell lines

Kalhori

Irani

Soleimani

et al. 2019

J of Cellular Biochemistry

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“…Studies evaluating these SNPs in pregnancy disorders are scarce. However, the BAX-248G/A SNP has attracted much attention to malignant disorders [36,40]. In this context, low levels of BAX expression in untreated CLL patients are associated with BAX-248GA/AA genotype carriers [39,40].…”

Section: Discussionmentioning

confidence: 99%

“…Currently, it is suggested that BAX-248G/A has no influence on cancer susceptibility [35,36]. The effect of SNP BAX-248G/A on the expression levels of the protein in CLL patients is still controversial [37,38].…”

Section: Introductionmentioning

confidence: 99%

The role of FAS, FAS-L, BAX, and BCL-2 gene polymorphisms in determining susceptibility to unexplained recurrent pregnancy loss

Michita

Zambra

Fraga

et al. 2019

J Assist Reprod Genet

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Purpose Idiopathic recurrent pregnancy loss (RPL) is a multifactorial reproductive disorder where an impaired control of apoptosis is likely involved. Triggering the cell death mechanism occurs in a spatiotemporal manner and is strongly related to a healthy pregnancy. Single nucleotide polymorphisms (SNPs) at the regulatory regions of genes are known to influence the expression patterns of apoptosis-related molecules. Methods A total of 296 unrelated female Brazilian patients were evaluated for clinical-demographic variables and genetic factors: 140 women who had experienced an unexplained RPL (with at least two consecutive abortions) and 156 healthy multiparous women. In all patients, six SNPs were evaluated in genes of apoptosis-related pathways: FAS (rs2234767, rs1800682), FAS-L (rs763110, rs5030772), BAX (rs4645878), and BCL-2 (rs2279115) by PCR followed by a restriction fragment length polymorphism (RFLP)-based analysis. Results The BAX-248GA genotype is independently associated with idiopathic RPL [adjusted OR = 0.30, 95% CI 0.13-0.70, P = 0.005] susceptibility. In the same multivariate model, the variables ethnicity, smoking, and alcohol consumption were statistically associated with RPL susceptibility (P < 0.05). No association with RPL susceptibility was reported for the remaining SNPs. Conclusion Our study is the first to evaluate the role of the main SNPs from both the extrinsic and intrinsic apoptosis pathways in RPL susceptibility. The association of BAX-248G/A with RPL susceptibility suggests that maternal predisposition for RPL has an essential contribution from genes involved in the delicate balance of endometrium cell turnover (cell death/proliferation). Therefore, apoptotic genes may represent promising targets for future studies on healthy pregnancies and the spectrum of pregnancy disorders.

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“…A systematic review and meta-analysis suggested that BAX gene polymorphisms were significantly associated with unfavorable prognosis in cancers. 27 As for CDKN1A, the mRNA and protein expression levels of CDKN1A were significantly up-regulated in many cancer tissues. The high expression of CDKN1A is associated with progression and poor prognosis of many tumors like breast cancer and gastric cancer.…”

Section: Discussionmentioning

confidence: 96%

“…BAX (BCL‐2 Associated X) is an important modulator of apoptosis. A systematic review and meta‐analysis suggested that BAX gene polymorphisms were significantly associated with unfavorable prognosis in cancers . As for CDKN1A, the mRNA and protein expression levels of CDKN1A were significantly up‐regulated in many cancer tissues.…”

Section: Discussionmentioning

confidence: 99%

Development and validation of autophagy‐related‐gene biomarker and nomogram for predicting the survival of cutaneous melanoma

Wan

Jin

et al. 2020

IUBMB Life

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Autophagy plays a critical role in cutaneous melanoma, but the prognostic research of differentially expressed autophagy‐related genes (DEARGs) in melanoma is lacking. Therefore, autophagy‐related gene expression data of 630 melanoma patients were obtained from Gene Expression Omnibus (GEO) and The Cancer Genome Atlas database. The DEARGs were identified by “limma” package in R software. Best survival analysis and the least absolute shrinkage and selection operator method were performed to identify a robust 7‐DEARG signature as well as construct nomogram associated with the clinical characteristics and validation in internal and external sets. This 7‐DEARG signature could be regarded as an independent prognostic signature in clinical setting, and nomogram may supply a more simple and accurate prediction for the prognosis of melanoma. Moreover, 5 cancer hallmarks and 18 potential compounds are commonly enriched in high‐risk expression phenotype. Thus, our finding provides new clinical evidences for the accurate diagnosis and identifies a potential prognostic autophagy‐related marker for the treatment of melanoma.

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Prognostic value and susceptibility of BAX rs4645878 polymorphism in cancer

Abstract: Supplemental Digital Content is available in the text

Cited by 13 publications

References 30 publications

The effect of miR‐579 on the PI3K/AKT pathway in human glioblastoma PTEN mutant cell lines

The effect of miR‐579 on the PI3K/AKT pathway in human glioblastoma PTEN mutant cell lines

The role of FAS, FAS-L, BAX, and BCL-2 gene polymorphisms in determining susceptibility to unexplained recurrent pregnancy loss

Development and validation of autophagy‐related‐gene biomarker and nomogram for predicting the survival of cutaneous melanoma

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