Prognostic significance of miR-218 in human hepatocellular carcinoma and its role in cell growth

Tu, Kangsheng; Li, Chao; Zheng, Xin; Yang, Wei; Yao, Yingmin; Liu, Qingguang

doi:10.3892/or.2014.3386

Cited by 31 publications

(28 citation statements)

References 26 publications

(52 reference statements)

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“…miRNAs that promote carcinogenesis are termed oncomiRs (7). Numerous oncomiRs are overexpressed in CRC compared with noncancerous tissues, including miR-494, miR-21, miR-23a and miR-130b (8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

miR-517a is an independent prognostic marker and contributes to cell migration and invasion in human colorectal cancer

Jiang

et al. 2016

Oncology Letters

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Abstract. Colorectal cancer (CRC) is a highly invasive tumor that is frequently associated with distant metastasis, which is the primary cause of poor prognosis. However, the mechanisms of metastasis remain poorly understood. MicroRNAs (miRNAs/miRs) have been considered to be implicated in CRC progression. In particular, miR-517a is proposed as a novel tumor-associated miRNA and has a potential role in tumor metastasis. The expression of miR-517a in CRC specimens was detected by reverse transcription-quantitative polymerase chain reaction. Transwell assays were performed to determine the migration and invasion of CRC cells. The putative target genes of miR-517a were disclosed using publicly available databases and western blot analysis. The present study identified that the expression of miR-517a was significantly higher in CRC tissues as compared with adjacent non-tumor tissues. Clinical analysis indicated that increased expression of miR-517a was correlated with poor prognostic features and poor long-term survival of CRC patients. In vitro evidences demonstrated that downregulation of miR-517a inhibited cell migration and invasion in HCT-116 cells. By contrast, upregulation of miR-517a increased the number of migrated and invaded SW480 cells. Notably, miR-517a expression was inversely regulated by forkhead box J3 (FOXJ3) abundance in CRC cells. Furthermore, an inverse correlation between miR-517a and FOXJ3 expression was observed in CRC tissues. In conclusion, miR-517a appears to be an independent prognostic marker for predicting survival of CRC patients, and may promote cell migration and invasion by inhibiting FOXJ3.

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Section: Introductionmentioning

confidence: 99%

miR-517a is an independent prognostic marker and contributes to cell migration and invasion in human colorectal cancer

Jiang

et al. 2016

Oncology Letters

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“…Recently, miR-218 is found to be downregulated in many human malignant tumors. In HCC, the low expression of miR-218 was reported to confer a poor 5-year survival in HCC patients and miR-218 may serve as a prognostic biomarker and induce apoptosis and growth arrest by downregulating BMI-1 in HCC (Tu et al, 2014). Likewise, reduced miR-218 in pancreatic ductal adenocarcinoma tissues was correlated with tumor progression, and might be an independent poor prognostic factor for patients (Zhu et al, 2014).…”

Section: Discussionmentioning

confidence: 89%

Expression of microRNA-218 and its Clinicopathological and Prognostic Significance in Human Glioma Cases

Cheng¹,

Wang²,

Hu³

2015

Asian Pacific Journal of Cancer Prevention

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Background: MicroRNAs are a class of noncoding RNAs which regulate multiple cellular processes during tumor development. The purpose of this report is to investigate the clinicopathological and prognostic significance of miR-218 in human gliomas. Materials and Methods: Quantitative RT-PCR (qRT-PCR) was conducted to detect the expression of miR-218 in primary normal human astrocytes, three glioma cell lines and 98 paired glioma and adjacent normal brain tissues.Associations of miR-218 with clinicopathological variables of glioma patients were statistically analyzed. Finally, a survival analysis was performed using the Kaplan-Meier method and Cox's proportional hazards model. Results: The expression level of miR-218 in primary normal human astrocytes was significantly higher than that in glioma cell lines (p<0.01). Also, the expression level of miR-218 in glioma tissues was significantly downregulated in comparison with that in the adjacent normal brain tissues (p<0.001). Statistical analyses demonstrated that low miR-218 expression was closely associated with advanced WHO grade (p=0.002) and low Karnofsky performance score (p=0.010) of glioma patients. Kaplan-Meier analysis with the log-rank test showed that patients with low-miR-218 expression had poorer disease-free survival and overall survival (p=0.0045 and 0.0124, respectively). Multivariate analysis revealed that miR-218 expression was independently associated with the disease-free survival (p=0.009) and overall survival (p=0.004) of glioma patients. Conclusions: Our results indicate that miR-218 is downregulated in gliomas and that its status might be a potential valuable biomarker for glioma patients.

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“…Low miR-218 expression was demonstrated to be associated with tumor cell invasion and migration, and poor prognosis (7,22,23). Furthermore, miR-218 expression is lower in malignant tumors compared with benign tumors (24). Low miR-218 expression is associated with tumor invasion, migration and poor prognosis (7,21–24).…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, miR-218 expression is lower in malignant tumors compared with benign tumors (24). Low miR-218 expression is associated with tumor invasion, migration and poor prognosis (7,21–24). Together, these studies suggest that miR-218 serves an important role in the development of tumors.…”

Section: Introductionmentioning

confidence: 99%

miRNA‑218 regulates the proliferation and apoptosis of cervical cancer cells via targeting Gli3

Zhang

et al. 2018

Exp Ther Med

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Cervical cancer (CC) is one of the most malignant tumors that affect women. Recent studies have reported that microRNAs (miRs) serve important roles in CC. The aim of the present study was to investigate the role of miR-218 in CC and to verify its underlying mechanism. The results of reverse transcription-quantitative polymerase chain reaction (RT-qPCR) revealed that miR-218 was dramatically downregulated in CC tissues and cell lines. Furthermore, the expression of Gli3 and Ki67 was measured using RT-qPCR and the results revealed that levels of these proteins were negatively correlated with miR-218 in CC tissues. The protein expression levels were determined by western blotting. Then SiHa cell line was used to investigate the mechanism of CC. Following cell transfection, cell apoptosis and cycle analyses were performed using the flow cytometry. The results revealed that miR-218 overexpression significantly inhibited cell proliferation, apoptosis and cell cycle. Additionally, luciferase reporter assay revealed that Gli3 may be a novel and direct target of miR-218 in CC. Taken together, the results of the present study suggest that miR-218 overexpression or Gli3 knockdown may have potential as therapeutic strategies for the treatment of CC.

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Prognostic significance of miR-218 in human hepatocellular carcinoma and its role in cell growth

Cited by 31 publications

References 26 publications

miR-517a is an independent prognostic marker and contributes to cell migration and invasion in human colorectal cancer

miR-517a is an independent prognostic marker and contributes to cell migration and invasion in human colorectal cancer

Expression of microRNA-218 and its Clinicopathological and Prognostic Significance in Human Glioma Cases

miRNA‑218 regulates the proliferation and apoptosis of cervical cancer cells via targeting Gli3

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