Prognostic significance of micrometastatic tumour cells in bone marrow of colorectal cancer patients

Lindemann, Fritz; Witte, Jens; Schlimok, Günter; Dirschedl, P.; Riethmüller, Gert

doi:10.1016/0140-6736(92)92230-d

Cited by 506 publications

(264 citation statements)

References 20 publications

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“…in a similar manner to micrometastatic tumour cells in bone marrow (Lindemann et al 1992;Soeth et al, 1997). Results of the present investigation suggest that circulating neoplastic cells correlate with the presence of distant metastasis at inclusion.…”

Section: Discussionmentioning

confidence: 93%

“…Cytokeratins, a multigene family of proteins with differentiationassociated patterns of expression (Moll et al, 1982), have also been used to characterize neoplastic cells of epithelial origin in bone marrow (Lindemann et al 1992) and peripheral blood (Denis et al, 1997;Nakamori et al, 1997;Soeth et al, 1997). However, most of them are found in samples of healthy subjects, thus limiting their suitability as targets (Burchill et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

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Detection of colonic cells in peripheral blood of colorectal cancer patients by means of reverse transcriptase and polymerase chain reaction

Castells

Boix²,

Bessa³

et al. 1998

Br J Cancer

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Detection of colonic cells in peripheral blood of colorectal cancer patients by means of reverse transcriptase and polymerase chain reaction

Castells

Boix²,

Bessa³

et al. 1998

Br J Cancer

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“…Detection of circulating tumor cells is of substantial clinical importance and the prognostic value of these results has been demonstrated (22). Our results show that SFM is a powerful method for finding the fluorescentlabeled tumor cells contributing to the analysis and solution of this clinical problem, and that SFM is equivalent to LSC and FCM.…”

Section: Discussionmentioning

confidence: 73%

Scanning fluorescent microscopy analysis is applicable for absolute and relative cell frequency determinations

et al. 2004

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Background: Flow cytometry (FCM) and laser scanning cytometry (LSC) are the routine techniques for fluorescent cell analysis. Recently, we developed a scanning fluorescent microscopy (SFM) technique. This study compares SFM to LSC (two slide-based cytometry, SBC, techniques) and FCM, in experimental and clinical settings. Methods: For the relative cell-frequency determinations, HT29 colorectal cancer cells and Ficoll separated blood mononuclear cells (FSBMCs) were serially diluted (from 1:1 to 1:1,000) and measured by each of the three techniques. For the absolute cell number determinations (only for SBC) FSBMCs were smeared on slides, then HT29 cells were placed on the slide with a micromanipulator (5-50 cells). Tumor cells circulating in the peripheral blood were isolated by magnetic separation from clinical blood samples of colorectal cancer patients. All samples were double-stained by CD45 ECD and CAM5.2 FITC antibodies. For slides,

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“…Lindeman et al [21] demonstrated the absence of cytokeratins in any types of cells of a normal lymph node except the metastatic cells from epithelial primary. He observed the CK expression on metastatic cells only in a lymph node which was secondary to epithelial primary tumors.…”

Section: Macrometastasesmentioning

confidence: 99%

Immunohistochemistry, A Valuable Tool in Detection of Cervical Lymph Node Micrometastases in Head and Neck Squamous Cell Carcinoma: A Prospective Study

Sharma

Mishra

Gupta

2012

Indian J Otolaryngol Head Neck Surg

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The regional failure after comprehensive clearance of neck metastasis and consequent pathological report of N0 disease has been reported fairly frequently. The role of recurrence of disease in the neck in the cases has been variously reported by different authors. The light microscopy does not detect the micrometastasis and the specimen is reported negative for metastasis. The presence of micrometastasis (the reason for neck failure) has been reported by many studies as 5-58 % (mean 19.6 %). These figures are significantly high. The present study was done to ascertain the micrometastasis after comprehensive neck clearance (pN 0 report). Two groups of patients were included in this study. (1) Group I included patients with N0 necks (80 patients). (2) Group II included Patients with N? necks (107 patients).We found that 20 % case were reported N0 (Group I) in light microscopy but on immunohistochemistry these were positive for disease. 15 % upstaging was reported in N? cases (Group 2). Immunohistochemistry has been more sensitive for cancer detection and has significantly changed the tumor staging and its consequent management.

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Prognostic significance of micrometastatic tumour cells in bone marrow of colorectal cancer patients

Cited by 506 publications

References 20 publications

Detection of colonic cells in peripheral blood of colorectal cancer patients by means of reverse transcriptase and polymerase chain reaction

Detection of colonic cells in peripheral blood of colorectal cancer patients by means of reverse transcriptase and polymerase chain reaction

Scanning fluorescent microscopy analysis is applicable for absolute and relative cell frequency determinations

Immunohistochemistry, A Valuable Tool in Detection of Cervical Lymph Node Micrometastases in Head and Neck Squamous Cell Carcinoma: A Prospective Study

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