Prognostic significance of bi/oligoclonality in childhood acute lymphoblastic leukemia as determined by polymerase chain reaction

Scrideli, Carlos Alberto; Defavery, Ricardo; Bernardes, José Eduardo; Tone, Luíz Gonzaga

doi:10.1590/s1516-31802001000500005

Cited by 8 publications

(1 citation statement)

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“…The explanation for the difference in oligoclonality findings may be due to the somatic mutation of the VH genes (8,9). Another explanation for bi/oligoclonality may be the presence of two cell populations in the bone marrow, due to two separate events or the formation of sub-clones (10,11).…”

Section: Discussionmentioning

confidence: 99%

The DG75 B-cell lymphoma line exhibits biclonal immunoglobulin gene rearrangement

et al. 2012

Biomedical Reports

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Abstract. Immunoglobulin (Ig) and T-cell receptor (TCR) gene rearrangement (GR) studies have been successfully employed to investigate the clonality and cell lineage of various lymphoid malignancies. Several lymphoma cell lines, such as BJAB, RAJI, DG75 and Jurkat cell lines, were often used as the positive controls in GR detection assays. Of those, the DG75 B-cell lymphoma line was found to exhibit biclonality [two or more homoduplex and heteroduplex bands in a polymerase chain reaction (PCR) product of clonality assay] in the PCR of GR detection assays. To further explore these characteristics of the biclonal phenomenon, the PCR products were purified and cloned into a pEGM-T clone vector. The sequences were analyzed using DNA analysis software. The results demonstrated that the two bands originated from two forms of GR of DG75 cell lines, i.e., DG75 is a biclonal cell line in Ig GRs, which has not been reported before.

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Section: Discussionmentioning

confidence: 99%