Prognostic significance of bcl-2, c-myc, survivin and tumor grade in synovial sarcoma

Demır, Derya; Yaman, Banu; Anacak, Yavuz; Keçeci, Burçin; Kandıloğlu, Gülşen

doi:10.5146/tjpath.2014.01164

Cited by 4 publications

(3 citation statements)

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“…Therefore, the apoptosis of hepatocellular carcinoma cells was substantially enhanced in the DEN + IVIG group in contrast to the DEN + NS group. In addition, the Bcl-2 family indirectly controls cell-autonomous death by interacting with the proto-oncogene c-myc [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Intravenous Immunoglobulin Inhibits Liver Cancer Progression by Promoting p38MAPK-Associated Apoptosis

Lin

Liu

et al. 2022

Journal of Oncology

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Objective. The aim of this study is to explore the effect of intravenous immunoglobulin (IVIG) on the development of rat hepatocellular carcinoma and its possible molecular mechanism. Methods. Sixty adult male Sprague-Dawley (SD) rats were randomly divided into three groups: control, diethylnitrosamine(DEN) + normal saline(NS), and DEN + IVIG groups, with 20 rats in each group. The rats in the DEN + NS group and DEN + IVIG group were given DEN 0.2 g/kg intraperitoneal injection once on day 1 and then 0.05% DEN aqueous solution in drinking water to establish a rat liver cancer model. Immunoglobulin (IgG) was injected intraperitoneally into the DEN + IVIG group twice a week at the dose of 100 mg/kg, and saline was administered intraperitoneally into the control group at a 50 mg/kg dosage. The body weight of each group of rats was recorded twice a week. All treatments were maintained continuously for 12 weeks. After the intervention, the liver function indexes of rats were measured by a fully automated biochemical analysis instrument. The liver histopathology was observed by hematoxylin-eosin(HE) staining. Immunohistochemistry was used to detect c-myc protein expression, and Western blotting was used to determine p38MAPK and p-p38MAPK protein expressions, as well as apoptosis-related proteins such as Bcl-2, Bax, and cleaved caspase-3. Results. Compared with the rats in the DEN + NS group, rats in the DEN + IVIG group showed substantially higher body mass ( P < 0.05 ), higher survival rate ( P < 0.05 ), and lower liver function indexes ( P < 0.05 ). Few focal necrosis of cancer cells and few nuclear division were observed in the rats in the DEN + IVIG group. The rats in the DEN + NS group showed lamellar necrosis of cancer foci, destruction of normal liver lobular structure, and hepatocellular carcinoma cells. Immunohistochemical analysis results revealed that the expression of c-myc was reduced in the DEN + IVIG group ( P < 0.05 ), and Western blotting confirmed that the Bcl-2 expression was decreased ( P < 0.05 ), while Bax, p38 MAPK, p-p38 MAPK, and cleaved caspase-3 protein expressions were increased ( P < 0.05 ). Conclusion. IVIG prophylactic injection can delay tumor development and induce apoptosis in primary hepatocellular carcinoma in rats. The mechanism is connected to the activation of the p38MAPK signaling pathway by upregulating the level of cleaved caspase-3 and Bax proteins while downregulating the level of Bcl-2 and c-myc proteins.

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Section: Discussionmentioning

confidence: 99%

Intravenous Immunoglobulin Inhibits Liver Cancer Progression by Promoting p38MAPK-Associated Apoptosis

Lin

Liu

et al. 2022

Journal of Oncology

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“…Immunohistochemical markers have also been investigated. Suggested antibodies that have been reported to be of prognostic value include Bcl-2 and c-myc [18], survivin [19], FGFR3 [20], secernin-1 [21], CXCR4, and IGF-1R [22]. In the study by Tarkan et al [23], high mitotic activity was significantly associated with worse disease-specific survival on multivariate analysis.…”

Section: Key Pointsmentioning

confidence: 93%

Synovial sarcoma diagnosis and management in the era of targeted therapies

Vlenterie

Jones

Graaf

2015

Current Opinion in Oncology

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“…Several lines of evidence suggest that c-Myc, a basic region/helix-loop-helix/leucine zipper (b/HLH/Zip) transcription factor, is an important oncogenic signalling molecule in RMS [37]. Indeed, upregulated levels of c-Myc is often associated with tumour aggression and poor clinical outcome [38][39][40][41][42][43][44][45][46][47]. Furthermore, c-Myc was shown to function as a pro-proliferative and anti-apoptotic factor in RMS by repressing p21, and when it was depleted in ERMS cell lines a number of metastatic, invasive and angiogenic markers decreased [42,48,49].…”

Section: Introductionmentioning

confidence: 99%

The c-Myc/AKT1/TBX3 Axis Is Important to Target in the Treatment of Embryonal Rhabdomyosarcoma

Sims

Maranyane

Damerell

et al. 2020

Cancers

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Rhabdomyosarcoma is a highly aggressive malignant cancer that arises from skeletal muscle progenitor cells and is the third most common solid tumour in children. Despite significant advances, rhabdomyosarcoma still presents a therapeutic challenge, and while targeted therapy has shown promise, there are limited options because the molecular drivers of rhabdomyosarcoma are poorly understood. We previously reported that the T-box transcription factor 3 (TBX3), which has been identified as a druggable target in many cancers, is overexpressed in rhabdomyosarcoma patient samples and cell lines. To identify new molecular therapeutic targets to treat rhabdomyosarcoma, this study investigates the potential oncogenic role(s) for TBX3 and the factors responsible for upregulating it in this cancer. To this end, rhabdomyosarcoma cell culture models in which TBX3 was either stably knocked down or overexpressed were established and the impact on key hallmarks of cancer were examined using growth curves, soft agar and scratch motility assays, as well as tumour-forming ability in nude mice. Our data show that TBX3 promotes substrate-dependent and -independent proliferation, migration and tumour formation. We further reveal that TBX3 is upregulated by c-Myc transcriptionally and AKT1 post-translationally. This study identifies c-Myc/AKT1/TBX3 as an important axis that could be targeted for the treatment of rhabdomyosarcoma.

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Prognostic significance of bcl-2, c-myc, survivin and tumor grade in synovial sarcoma

Cited by 4 publications

References 0 publications

Intravenous Immunoglobulin Inhibits Liver Cancer Progression by Promoting p38MAPK-Associated Apoptosis

Intravenous Immunoglobulin Inhibits Liver Cancer Progression by Promoting p38MAPK-Associated Apoptosis

Synovial sarcoma diagnosis and management in the era of targeted therapies

The c-Myc/AKT1/TBX3 Axis Is Important to Target in the Treatment of Embryonal Rhabdomyosarcoma

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