Prognostic significance of apoptosis in medulloblastoma

Pižem, Jože; Cör, Andrej; Zaletel, Lorna Zadravec; Popović, Mara

doi:10.1016/j.neulet.2005.02.021

Cited by 14 publications

(7 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3a). The tumor tissue had higher levels of apoptosis compared to non-tumor tissue as reported before in this mouse model as well as in human medulloblastoma [10,40,47], as determined by cleaved PARP levels, indicating good separation between tumor and non-tumor tissue.…”

Section: Resultssupporting

confidence: 72%

An essential role for p38 MAPK in cerebellar granule neuron precursor proliferation

et al. 2012

View full text Add to dashboard Cite

Development of the cerebellum occurs postnatally and is marked by a rapid proliferation of cerebellar granule neuron precursors (CGNPs). CGNPs are the cells-of-origin for SHH-driven medulloblastoma, the most common malignant brain tumor in children. Here, we investigated the role of ERK, JNK, and p38 mitogen-activated protein kinases (MAPKs) in CGNP proliferation. We found high levels of p38α in proliferating CGNPs. Concomitantly, members of the p38 pathway, such as ASK1, MKK3 and ATF-2, were also elevated. Inhibition of the Shh pathway or CGNP proliferation blunts p38α levels, irrespective of Shh treatment. Strikingly, p38α levels were high in vivo in the external granule layer (EGL) of the postnatal cerebellum, Shh-dependent mouse medulloblastomas and human medulloblastomas of the SHH subtype. Finally, knocking down p38α by short hairpin RNA-carrying lentiviruses as well as the pharmacologically inhibiting of its kinase activity caused a marked decrease in CGNP proliferation, underscoring its requirement for Shh-dependent proliferation in CGNPs. The inhibition of p38α also caused a decrease in Gli1 and N-myc transcript levels, consistent with reduced proliferation. These findings suggest p38 inhibition as a potential way to increase the efficacy of treatments available for malignancies associated with deregulated SHH signaling, such as basal cell carcinoma and medulloblastoma.

show abstract

Section: Resultssupporting

confidence: 72%

An essential role for p38 MAPK in cerebellar granule neuron precursor proliferation

et al. 2012

View full text Add to dashboard Cite

show abstract

“…The Wrst sections were routinely stained with haematoxylin and eosin for tissue morphology assessment and determination of MPM histological type according to the World Health Organization ICD-O (Churg et al 2004). The remaining sections were used for survivin immunohistochemical staining as previously described (Pizem et al 2005b). BrieXy, a 5-m tissue section of each tumour specimen was placed on a poly-L-lysine coated slide, de-paraYnised and rehydrated.…”

Section: Histological Analysismentioning

confidence: 99%

BIRC5 promoter SNPs do not affect nuclear survivin expression and survival of malignant pleural mesothelioma patients

Hmeljak

Erčulj

Dolžan

et al. 2011

J Cancer Res Clin Oncol

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is expressed in the proliferative phase of the cell cycle [9]. Many studies have proved the involvement of survivin into pathogenesis of different kinds of tumors, including tumors of the CNS [9, 10]. Expression of survivin could be an early event in tumorogenesis [11].…”

Section: Introductionmentioning

confidence: 99%

Caspase-3 and Survivin Expression in Primary Atypical and Malignant Meningiomas

Vranic

2013

ISRN Neuroscience

View full text Add to dashboard Cite

Objective. Information about possible prognostic factors of the survival of patients with atypical and malignant meningiomas (AMM) is sparse. The aim of our study was to evaluate prognostic significance of apoptotic marker caspase-3 and apoptotic inhibitor survivin in a series of primary AMM. Methods. 86 AMM (76 atypical and 10 malignant) were analyzed. Caspase-3 and survivin expression was evaluated immunohistochemically. The correlation between caspase-3, survivin, and other possible factors of meningioma recurrence was evaluated. Uni- and multivariate recurrence-free survival (RFS) and overall survival (OS) analyses were performed. Results. The intensity of caspase-3 expression correlated with the tumor grade (P = 0.004), the proliferation index (P = 0.019), and the mitotic count (P = 0.013). Survivin tended to be more expressed in female patients (P = 0.072). Survivin expression was stronger in malignant compared to atypical meningiomas, however, the difference was not statistically important (P = 0.491). Neither survivin nor caspase-3 expression significantly predicted OS or RFS in patients with AMM. Conclusions. Strong caspase-3 expression on AMM cells could reflect a cellular attempt at the homeostatic autoregulation of the tumor size. Survivin expression on AMM cells is similar to the survivin expression reported on benign meningiomas. Caspase-3 and survivin expression has no prognostic significance on the survival of patients with AMM.

show abstract

Prognostic significance of apoptosis in medulloblastoma

Cited by 14 publications

References 18 publications

An essential role for p38 MAPK in cerebellar granule neuron precursor proliferation

An essential role for p38 MAPK in cerebellar granule neuron precursor proliferation

BIRC5 promoter SNPs do not affect nuclear survivin expression and survival of malignant pleural mesothelioma patients

Caspase-3 and Survivin Expression in Primary Atypical and Malignant Meningiomas

Contact Info

Product

Resources

About