Prognostic significance of ANO1 expression in cancers

Zhang, Congxiao; Li, Haini; Gao, Jing; Cui, Xiaoqing; Yang, Shuai; Liu, Zongtao

doi:10.1097/md.0000000000024525

Cited by 9 publications

(6 citation statements)

References 37 publications

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“…According to ROC analysis and due to the significant correlation of TMEM expression with patient survival (OS and DFS), our study showed that these THEMs could serve as prognostic and diagnostic biomarkers in HNSCC. In meta-analysis involving 1760 patients from 7 independent studies, Zhang et al demonstrated the potential prognostic value of ANO1 in multiple cancer types, including HNSCC [ 41 ]. Although higher ANO1 expression is associated with worse OS and DFS, an in vitro model of HNSCC demonstrated that overexpression of ANO1 in Te11 cells was linked with enhanced sensitivity to EGFR -targeted agent, Gefitinib, making ANO1 a good predictive biomarker and potential target for EGFR -directed therapy [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

The Landscape of Transmembrane Protein Family Members in Head and Neck Cancers: Their Biological Role and Diagnostic Utility

Koteluk

Bielicka

Lemańska

et al. 2021

Cancers

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Background: Transmembrane proteins (TMEM) constitute a large family of proteins spanning the entirety of the lipid bilayer. However, there is still a lack of knowledge about their function or mechanism of action. In this study, we analyzed the expression of selected TMEM genes in patients with head and neck squamous cell carcinoma (HNSCC) to learn their role in tumor formation and metastasis. Materials and Methods: Using TCGA data, we analyzed the expression levels of different TMEMs in both normal and tumor samples and compared those two groups depending on clinical-pathological parameters. We selected four TMEMs whose expression was highly correlated with patient survival status and subjected them to further analysis. The pathway analysis using REACTOME and the gene set enrichment analysis (GSEA) were performed to evaluate the association of those TMEMs with genes involved in hallmarks of cancer as well as in oncogenic and immune-related pathways. In addition, the fractions of different immune cell subpopulations depending on TMEM expression were estimated in analyzed patients. The results for selected TMEMs were validated using GEO data. All analyses were performed using the R package, Statistica, and Graphpad Prism. Results: We demonstrated that 73% of the analyzed TMEMs were dysregulated in HNSCC and depended on tumor localization, smoking, alcohol consumption, or HPV infection. The expression levels of ANO1, TMEM156, TMEM173, and TMEM213 correlated with patient survival. The four TMEMs were also upregulated in HPV-positive patients. The elevated expression of those TMEMs correlated with the enrichment of genes involved in cancer-related processes, including immune response. Specifically, overexpression of TMEM156 and TMEM173 was associated with immune cell mobilization and better survival rates, while the elevated ANO1 expression was linked with metastasis formation and worse survival. Conclusions: In this work, we performed a panel of in silico analyses to discover the role of TMEMs in head and neck squamous cell carcinoma. We found that ANO1, TMEM156, TMEM173, and TMEM213 correlated with clinical status and immune responses in HNSCC patients, pointing them as biomarkers for a better prognosis and treatment. This is the first study describing such the role of TMEMs in HNSCC. Future clinical trials should confirm the potential of those genes as targets for personalized therapy of HNSCC.

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Section: Discussionmentioning

confidence: 99%

The Landscape of Transmembrane Protein Family Members in Head and Neck Cancers: Their Biological Role and Diagnostic Utility

Koteluk

Bielicka

Lemańska

et al. 2021

Cancers

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“…The results indicated that the three genes in the risk model were importantly involved in the regulation of tumor stemness. ANO1 was found to be a risk factor in many cancer types (HR = 1.52, 95% CI: 1.19-1.92), and was suggested to be a prognostic factor ( Zhang et al, 2021 ). Kim et al uncovered that ANO1 knockdown could increase the survival and inhibit local invasion of glioblastoma stem cells (GSCs) in mouse model, indicating that ANO1 was important in the maintenance of stemness ( Kim et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Exploring the role of tumor stemness and the potential of stemness-related risk model in the prognosis of intrahepatic cholangiocarcinoma

Yue

Tao

et al. 2023

Front. Genet.

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Background: Tumor stem cells (TSCs) have been widely reported to play a critical role in tumor progression and metastasis. We explored the role of tumor stemness in intrahepatic cholangiocarcinoma (iCCA) and established a prognostic risk model related to tumor stemness for prognosis prediction and clinical treatment guidance in iCCA patients.Materials and Methods: The expression profiles of iCCA samples (E-MTAB-6389 and GSE107943 cohorts) were used in the study. One-class logistic regression algorithm calculated the mRNA stemness index (mRNAsi). The mRNAsi-related genes were used as a basis for the identification of mRNAsi-related molecular subtypes through consensus clustering. The immune characteristics and biological pathways of different subtypes were assessed. The mRNAsi-related risk model was constructed with differentially expressed genes (DEGs) between subtypes.Results: The patients with high mRNAsi had longer overall survival than that with low mRNAsi. Two subtypes were identified with that C2 had higher mRNAsi and better prognosis than C1. Tumor-related pathways such as TGF-β and epithelial-mesenchymal transition (EMT) were activated in C1. C1 had higher enrichment of cancer-associated fibroblasts and tumor-associated macrophages, as well as higher immune response and angiogenesis score than C2. We screened a total 98 prognostic DEGs between C1 and C2. Based on the prognostic DEGs, we constructed a risk model containing three genes (ANO1, CD109, and CTNND2) that could divide iCCA samples into high- and low-risk groups. The two groups had distinct prognosis and immune characteristics. Notably, the risk score was negatively associated with mRNAsi (R = −0.53). High-risk group had higher enrichment score of T cell inflamed GEP, INF-γ, and cytolytic activity, and lower score of estimated IC50 of 5-fluorouracil and cisplatin than low-risk group.Conclusions: This study clarified the important role of tumor stemness in iCCA and developed an mRNAsi-related risk model for predicting the prognosis and supporting the clinical treatment in iCCA patients. The three genes (ANO1, CD109, and CTNND2) may serve as potential targets for iCCA treatment.

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“…The ANO1 channel (TMEM16A) is involved in cancer cell proliferation, invasion, survival, and apoptosis; the signaling pathways that can be regulated by ANO1 include ERK, PI3K-Akt, TNF, Ca-M, and EGFR [118,220,221]. Studies performed in head and neck squamous cell carcinoma (HNSCC) indicate that a high expression of ANO1 is related to metastatic processes in lymph nodes, poor prognosis, and the poor survival of patients with OSCC and ESCC [96,97,171,172].…”

Section: Calcium-activated Chloride Channelsmentioning

confidence: 99%

Ion Channels as Potential Tools for the Diagnosis, Prognosis, and Treatment of HPV-Associated Cancers

Chiliquinga,

Acosta,

Ogonaga-Borja

et al. 2023

Cells

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The human papilloma virus (HPV) group comprises approximately 200 genetic types that have a special affinity for epithelial tissues and can vary from producing benign symptoms to developing into complicated pathologies, such as cancer. The HPV replicative cycle affects various cellular and molecular processes, including DNA insertions and methylation and relevant pathways related to pRb and p53, as well as ion channel expression or function. Ion channels are responsible for the flow of ions across cell membranes and play very important roles in human physiology, including the regulation of ion homeostasis, electrical excitability, and cell signaling. However, when ion channel function or expression is altered, the channels can trigger a wide range of channelopathies, including cancer. In consequence, the up- or down-regulation of ion channels in cancer makes them attractive molecular markers for the diagnosis, prognosis, and treatment of the disease. Interestingly, the activity or expression of several ion channels is dysregulated in HPV-associated cancers. Here, we review the status of ion channels and their regulation in HPV-associated cancers and discuss the potential molecular mechanisms involved. Understanding the dynamics of ion channels in these cancers should help to improve early diagnosis, prognosis, and treatment in the benefit of HPV-associated cancer patients.

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Prognostic significance of ANO1 expression in cancers

Cited by 9 publications

References 37 publications

The Landscape of Transmembrane Protein Family Members in Head and Neck Cancers: Their Biological Role and Diagnostic Utility

The Landscape of Transmembrane Protein Family Members in Head and Neck Cancers: Their Biological Role and Diagnostic Utility

Exploring the role of tumor stemness and the potential of stemness-related risk model in the prognosis of intrahepatic cholangiocarcinoma

Ion Channels as Potential Tools for the Diagnosis, Prognosis, and Treatment of HPV-Associated Cancers

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