Prognostic significance of ADAM17 expression in patients with gastric cancer who underwent curative gastrectomy

Aydın, Dinçer; Bılıcı, Ahmet; Yavuzer, Dilek; Kefeli, Umut; Tan, Angelina D.; Ercelep, Özlem; Mert, Aslıhan Güven; Yuksel, Sinemis; Özçelik, Melike; Işik, Deniz; Sürmeli, Heves; Odabasi, H.; Aliustaoğlu, Mehmet

doi:10.1007/s12094-015-1283-1

Cited by 21 publications

(17 citation statements)

References 16 publications

(35 reference statements)

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“…ADAM17 has a role in many biological processes of multiple tumors [2,3]. In previous studies, ADAM17 is an important regulator of the tumorigenic properties of human NSCLC and may be used as a potential anticancer therapeutic target in NSCLC [4].…”

Section: Introductionmentioning

confidence: 98%

Adam17, a Target of Mir-326, Promotes Emt-Induced Cells Invasion in Lung Adenocarcinoma

Cai

Wang²,

Zhang³

et al. 2015

Cell Physiol Biochem

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Background/Aims: A disintegrin and metalloprotease (ADAM) 17 has been reported to be implicated in cancer cells invasion. Nevertheless, its potential role in lung adenocarcinoma has not been addressed clearly. Methods: RT-PCR and Western blot were used to detect the expression of miR-326 and ADAM17 in lung adenocarcinoma samples (n=73). miR-326 mimics and inhibitor were tansfected in human A549 and SPCA1 cell lines. The transwell assay was used to detect the cell invasive ability. The regulation mechanism was evaluated by luciferase reporter assay. The markers of (epithelial-to-mesenchymal transition) EMT were detected by using Western blot assay. Results: We found increased expression of ADAM17 in lung adenocarcinoma and cell lines. In vitro, up-regulation of ADAM17 promoted cells invasion, while silencing of ADAM17 inhibited cells invasion. Meanwhile, ADAM17 could affect the markers of EMT. Furthermore, we confirmed that ADAM17 is a target of miR-326, which is involved in EMT and cells invasion. Conclusions: These findings revealed that ADAM17, a target of miR-326, promoted EMT-induced cells invasion in lung adenocarcinoma.

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Section: Introductionmentioning

confidence: 98%

Adam17, a Target of Mir-326, Promotes Emt-Induced Cells Invasion in Lung Adenocarcinoma

Cai

Wang²,

Zhang³

et al. 2015

Cell Physiol Biochem

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“…In fact, as many as 76 proteins have been shown to be substrates for ADAM17 shedding activity, thus regulating responses to tissue injury, inflammation, and carcinogenesis [ 13 ]. It is indispensable regulator of cellular events from proliferation to migration [ 14 ]. The high expression of ADAM17 genes is poor prognostic factor in various cancer types and correlates with tumor progression (e.g., breast, prostate, gastric, colorectal, hepatocellular, and ovarian cancer) [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Expression of Migration-Related Genes in Human Colorectal Cancer and Activity of a Disintegrin and Metalloproteinase 17

Walkiewicz

Kozieł

Bednarczyk

et al. 2016

BioMed Research International

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Introduction. The ability to form metastases which depends on the mechanisms of cell migration is an important element of the progression of cancer. In the present study we analyzed the genes involved in the regulation of migration in colon cancer cells. Materials and Methods. A total of 20 pairs of surgically removed tumoral and healthy (marginal) tissues samples from colorectal cancer patients at clinical stages I-II and III-IV were analyzed. The isolation of RNA from CRC and normal tissues and its subsequent molecular analysis were performed according to manufacturer's instructions. Microarray data analysis was performed using the GeneSpring 11.5 platform and Significance Analysis of Microarrays (SAM). In SAM analysis to identify significantly differentially expressed genes score and q-value parameters were used. Results. The largest increase in expression of genes was shown by MMP9, ADAM17, EphA2, and TIMP. Conclusions. Presented genes, especially ADAM17, MMP9, EphA2, TIMP1, ICAM 11, and CD4, may be used as prognostic markers of advanced stages of colorectal cancer, contributing to the development of new lines of therapy focused on reducing metastasis of the primary tumor.

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“…Although the accurate source of sPD-L1 remains unclear, sPD-L1 might be released or shed from PD-L1-positive tumor cells. Considering that some of disintegrin and metalloproteinase (ADAM) proteases, transmembrane protein shedding enzymes, are overexpressed in undifferentiated GC tissues (34), enhanced ADAM activity might contribute to sPD-L1 production in OCUM-1 cells. It is also possible that matrix metalloproteinases might partly be associated with sPD-L1 release (35).…”

Section: Discussionmentioning

confidence: 99%

Interferon‑γ induced PD‑L1 expression and soluble PD‑L1 production in gastric cancer

et al. 2020

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Programmed death-ligand 1 (PD-L1) plays an essential role in tumor cell escape from anti-tumor immunity in various types of cancer, including gastric cancer (GC). The present study investigated the intracellular and membrane-bound expression of PD-L1 in the GC cell lines MKN1, MKN74, KATO III and OCUM-1. Furthermore, soluble PD-L1 (sPD-L1) level in the supernatant of GC cells and the serum of patients with GC and healthy controls was determined by ELISA. Interferon (IFN)-γ treatment of cells resulted in increased cytoplasmic expression of PD-L1 in GC cells in a dose-dependent manner, except for MKN74 cells; however, there was no association between tumor necrosis factor-α treatment and enhanced PD-L1 expression. Concordant with these findings, results from flow cytometry analysis demonstrated that membrane-bound PD-L1 expression was also increased following GC cell treatment with IFN-γ in a dose-dependent manner. In addition, significant sPD-L1 overproduction was observed only in the culture supernatant of OCUM-1 cells. Serum level of sPD-L1 was significantly increased in patients with GC, in particular in stage IV patients, compared with healthy controls. In conclusion, the present study demonstrated that IFN-γ treatment increased the intracellular and membrane-bound PD-L1 expression in GC cells. In addition, sPD-L1 was detected not only in the supernatant of GC cells but also in the serum of patients with GC. Further investigation on the underlying mechanism of regulation of PD-L1 expression and sPD-L1 production is required.

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Prognostic significance of ADAM17 expression in patients with gastric cancer who underwent curative gastrectomy

Abstract: The potential value of ADAM17 expression as a useful molecular marker in gastric cancer progression should be evaluated comprehensively; it may predict recurrence and poor prognosis in patients with gastric cancer after curative resection.

Cited by 21 publications

References 16 publications

Adam17, a Target of Mir-326, Promotes Emt-Induced Cells Invasion in Lung Adenocarcinoma

Adam17, a Target of Mir-326, Promotes Emt-Induced Cells Invasion in Lung Adenocarcinoma

Expression of Migration-Related Genes in Human Colorectal Cancer and Activity of a Disintegrin and Metalloproteinase 17

Interferon‑γ induced PD‑L1 expression and soluble PD‑L1 production in gastric cancer

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