Prognostic Signature GXYLT2 Is Correlated with Immune Infiltration in Bladder Cancer

Abstract

Background. GXYLT2 (glucoside xylosyltransferase 2) was known as an important gene that regulates classical Notch signaling and is involved in progression in human tumors. However, the correlation between GXYLT2 expression and bladder cancer remains unclear. Methods. GXYLT2 expression was analyzed by ONCOMINE database, GEPIA database, and TIMER database. The Cancer Genome Atlas (TCGA) was utilized to confirm relationships between GXYLT2 and molecular subtypes of BLCA (bladder cancer). We discovered prognostic value of GXYLT2 in BLCA using GEPIA, LinkedOmics database, and Kaplan-Meier Plotter database. Subsequently, correlations between GXYLT2 and tumor immune infiltration were investigated through TIMER and TISIDB website. We then downloaded data of patients with BLCA from TCGA website, to conduct functional annotations and to construct protein-protein interaction network through STRING and Enrich web servers. Results. Significant differences were observed between GXYLT2 expression of bladder cancer and normal tissues. GXYLT2 was a poor prognostic biomarker in BLCA with impact on diverse clinical characteristics. We found that GXYLT2 was closely related to tumor immune infiltrated cells and immune genes. Functional annotations indicated that GXYLT2 was linked to immune-related pathways. Conclusions. The results suggested that GXYLT2 was associated with a poor prognosis and tumor immune cell infiltration of BLCA. GXYLT2 could be a promising therapeutic target in bladder cancer.

Exploration of lncRNA/circRNA-miRNA-mRNA network in patients with chronic atrophic gastritis in Tibetan plateau areas based on DNBSEQ-G99 RNA sequencing

“…Disruption of GR signaling can induce spontaneous gastric inflammation and chemosis in female mice 39 . GXYLT2 is considered an important gene that regulates canonical Notch signaling, participates in human tumor progression, and is closely related to tumor immune infiltrating cells and immune genes 40 , 41 . Zhang et al 42 showed that high levels of TMC5 are activators of PI3K/AKT, RAS/MAPK, and TSC/mTOR, and it has been reported that activation of these pathways may lead to dysregulation of cell survival and ultimately tumorigenesis 43 and various cancers 44 .…”

Advancing Biomarker Discovery and Therapeutic Targets in Duchenne Muscular Dystrophy: A Comprehensive Review

“…Glucoside Xylosyltransferase 2 ( gxylt2 ) regulates the activity of the Notch pathway and determines the phosphorylation of MAPK, accelerating cell growth and migration of human cancer cells [ 98 ]. Intriguingly, other studies showed that gxylt2 was correlated to negative prognosis and immune infiltration in bladder cancer [ 99 ], and its methylation was associated with epithelial cells in the inflamed colon of patients suffering from ulcerative colitis [ 100 ]. Transcription elongation factor A (SII)-like 9 ( tceal9 ) and 8 ( tceal8 ) modulate transcription and protein-protein interactions [ 101 ].…”

Pan-cancer analysis of the prognostic significance and oncogenic role of GXYLT2

“…[4] In human bladder cancer, GXYLT2 was closely associated with tumor immune cell infiltration and immune gene expression. [5] In in vitro studies, GXYLT2 overexpression in human breast and gastric cancer cells upregulated Notch/MARK signaling and accelerated cell proliferation and migration. [6] Nonetheless, the prognostic significance and oncogenic role of GXYLT2 in most cancer types remain unclear.…”