Prognostic role of PHYH for overall survival (OS) in clear cell renal cell carcinoma (ccRCC)

Abstract

This study attempts to evaluate the prognostic role of PHYH for overall survival (OS) in clear cell renal cell carcinoma (ccRCC) by means of publicly available data from The Cancer Genome Atlas (TCGA). Clinical pathologic features and PHYH expression were downloaded from the TCGA database and relationships between them were analyzed by univariate and multivariate Cox regression analyses. Gene Set Enrichment Analysis (GSEA) and gene–gene interactions were also performed between tissues with different PHYH expression levels. PHYH expression levels were significantly lower in patient with ccRCC compared with normal tissues (p = 1.156e−19). Kaplan–Meier survival analysis showed that high expression of PHYH had a better prognosis than low expression (p = 9e−05). Moreover, PHYH expression was also significantly associated with high grade (G2-4, p = 0.025), high stage (StageIII & IV, p = 5.604e−05), and high level of stage_T (T3-4, p = 4.373e−05). Univariate and multivariate Cox regression analyses indicated that PHYH could be acted as an independent prognostic factor (p < 0.05). Nomogram including clinical pathologic features and PHYH expression were also provided. GSEA revealed that butanoate metabolism, histidine metabolism, propanoate metabolism, pyruvate metabolism, tryptophan metabolism, PPAR signalling pathway, and renin–angiotensin system were differentially enriched in PHYH high-expression phenotype. ICGC database was utilized to verify the expression level and survival benefit of PHYH (both p < 0.05). We suspect that elevated PHYH expression may be served as a potential prognostic molecular marker of better survival in ccRCC. Besides, alpha-oxidation was closely regulated by PHYH, and PPAR signalling, pyruvate metabolism, butanoate metabolism, and RAS might be the key pathways regulated by PHYH in CCRC.

An Investigation of the Prognostic Role of Genes Related to Lipid Metabolism in Head and Neck Squamous Cell Carcinoma

“…The literature also indicates that TPTE, INMT, and STAR are differentially expressed in tumours and may be associated with prognosis [46][47][48][49][50]. PHYH has been proven to be linked to multiple diseases, such as Refsum disease and retinitis pigmentosa, and can serve as a prognostic marker in clear cell renal cell carcinoma [51,52]. Moreover, we showed that PHYH was a cancer-promoting gene in HNSCC, a view that is consistent with Xiong et al [53].…”

Topical Application of Temperature-Sensitive Gel Containing Caerin 1.1 and 1.9 Peptides on TC-1 Tumour-Bearing Mice Induced High-Level Immune Response in the Tumour Microenvironment

“…It functions via the interaction with Irf9 to activate the interferon-stimulated genes in the nucleus, thereby enhancing the cellular immunity ( 104 ), while the elevation of Irf9 content was exclusively detected in the caerin group. Phyh (peroxisomal phytanoyl-CoA dioxygenase) regulates peroxisomal fatty acid β-oxidation metabolism and ROS conversion ( 105 ); its concentration positively correlates with potential tumour suppressive environment ( 106 , 107 ), working synergistically with iNOS induced by Stat1 , which may trigger the recruitment of CTLs ( 108 , 109 ). This appeared consistent with more activated CD8 + T cells detected by scRNA-seq.…”

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“…It functions via the interaction with Irf9 to activate the interferonstimulated genes in the nucleus, thereby enhancing the cellular immunity (104), while the elevation of Irf9 content was exclusively detected in the caerin group. Phyh (peroxisomal phytanoyl-CoA dioxygenase) regulates peroxisomal fatty acid b-oxidation metabolism and ROS conversion (105); its concentration positively correlates with potential tumour suppressive environment (106,107), working synergistically with iNOS induced by Stat1, which may trigger the recruitment of CTLs (108,109). This appeared consistent with more activated CD8 + T cells detected by scRNA-seq.…”