Prognostic role of circulating tumor cells in patients with <i>EGFR</i>‐mutated or <i>ALK</i>‐rearranged non‐small cell lung cancer

Tong, Bing; Xu, Yan; Zhao, Jing; Chen, Minjiang; Zhong, Wei; Xing, Jia; Wang, Mengzhao

doi:10.1111/1759-7714.12631

Cited by 20 publications

(13 citation statements)

References 20 publications

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“…Such data support the notion that CTCs can simultaneously provide an overview of tumor genomics and gene expression, and that the characterization of CTCs may be a key factor as these cells are ultimately responsible for metastasis [90]. CTC counts work as independent prognostic factors at both baseline and during follow-up in patients with EGFR-mutated or ALK-rearranged NSCLCs undergoing standard chemotherapy or targeted therapies [102]. In patients with metastatic cancer receiving systemic treatment, temporal changes in the number of CTCs have been shown to correlate with clinical outcome, as measured by standard radiography [103].…”

Section: Liquid Biopsy In Monitoring Response To Treatmentsupporting

confidence: 56%

Liquid Biopsy for the Detection of Resistance Mechanisms in NSCLC: Comparison of Different Blood Biomarkers

Pasini

Ulivi

2019

JCM

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The use of targeted agents and immunotherapy for the treatment of advanced non-small-cell lung cancer (NSCLC) has made it mandatory to characterize tumor tissue for patient selection. Moreover, the development of agents that are active against specific resistance mechanisms arising during treatment make it equally important to characterize the tumor tissue at progression by performing tissue re-biopsy. Given that tumor tissue is not always available for molecular characterization due to the paucity of diagnostic specimens or problems relating to the carrying out of invasive procedures, the use of liquid biopsy represents a valid approach to overcoming these difficulties. The most common material used for liquid biopsy in this setting is plasma-derived cell free DNA (cfDNA), which originates from cells undergoing apoptosis or necrosis. However, other sources of tumor material can be considered, such as extracellular vesicle (EV)-derived nucleic acids, which are actively secreted from living cells and closely correspond to tumor dynamics. In this review, we discuss the role of liquid biopsy in the therapeutic management of NSCLC with particular regard to targeted therapy and immunotherapy, and analyze the pros and cons of the different types of samples used in this context.

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Section: Liquid Biopsy In Monitoring Response To Treatmentsupporting

confidence: 56%

Liquid Biopsy for the Detection of Resistance Mechanisms in NSCLC: Comparison of Different Blood Biomarkers

Pasini

Ulivi

2019

JCM

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“…In many types of cancer, including osteosarcoma, overexpression of EGFR is associated with aggressive invasiveness and resistance to therapy (24)(25)(26). Previous studies have indicated that EGFR may be an effective target for the treatment of human cancer (27,28). The US Food and Drug Administration has approved five anti-EGFR agents for cancer treatment including three small molecule inhibitors and two .…”

Section: Discussionmentioning

confidence: 99%

Ursolic acid suppresses the biological function of osteosarcoma cells

et al. 2019

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Osteosarcoma is a highly malignant tumour that occurs in adolescents. Upregulation or the constitutive activation of epidermal growth factor receptor (EGFR) is a hallmark of osteosarcoma. To investigate the effect of ursolic acid on the biological function of osteosarcoma, MTT assay was used to detect the effect of ursolic acid on the proliferation of HOS and MG63 cells, while flow cytometry was used to analyse the effect on the cell cycle and apoptosis. Transwell and Matrigel assays were used to detect the effect of ursolic acid on cell migration and invasion, respectively. Western blot analysis and reverse transcription-quantitative polymerase chain reaction were used to detect the effects of different concentrations of ursolic acid on EGFR signaling pathway-related proteins, cell cycle, apoptosis and cell migration-related proteins. After overexpression or silencing of EGFR, the effects of ursolic acid on EGFR pathway and cell biological function were subsequently detected, using the same methods. The present study identified that ursolic acid had inhibitory effects on the growth and metastatic ability of osteosarcoma cells by suppressing EGFR.

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“…This is in accordance with the progressive discovery of different mutations in several genes involved in different biological processes regulating lung cancer development and progression. As an example, epidermal growth factor receptor (EGFR) [29], anaplastyc lymphoma kinase (ALK) [30], and Kirsten rat sarcoma viral oncogene homolog (K-RAS) [31] mutations, are considered prognostic and predictive biomarkers and targets for personalized drugs [32, 33]. In this context, the use of miRNA therapeutics as “next generation targeted drugs” seems to be promising [34].…”

Section: Resultsmentioning

confidence: 99%

MiRNA therapeutics based on logic circuits of biological pathways

et al. 2019

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BackgroundIn silico experiments, with the aid of computer simulation, speed up the process of in vitro or in vivo experiments. Cancer therapy design is often based on signalling pathway. MicroRNAs (miRNA) are small non-coding RNA molecules. In several kinds of diseases, including cancer, hepatitis and cardiovascular diseases, they are often deregulated, acting as oncogenes or tumor suppressors. miRNA therapeutics is based on two main kinds of molecules injection: miRNA mimics, which consists of injection of molecules that mimic the targeted miRNA, and antagomiR, which consists of injection of molecules inhibiting the targeted miRNA. Nowadays, the research is focused on miRNA therapeutics. This paper addresses cancer related signalling pathways to investigate miRNA therapeutics.ResultsIn order to prove our approach, we present two different case studies: non-small cell lung cancer and melanoma. KEGG signalling pathways are modelled by a digital circuit. A logic value of 1 is linked to the expression of the corresponding gene. A logic value of 0 is linked to the absence (not expressed) gene. All possible relationships provided by a signalling pathway are modelled by logic gates. Mutations, derived according to the literature, are introduced and modelled as well. The modelling approach and analysis are widely discussed within the paper. MiRNA therapeutics is investigated by the digital circuit analysis. The most effective miRNA and combination of miRNAs, in terms of reduction of pathogenic conditions, are obtained. A discussion of obtained results in comparison with literature data is provided. Results are confirmed by existing data.ConclusionsThe proposed study is based on drug discovery and miRNA therapeutics and uses a digital circuit simulation of a cancer pathway. Using this simulation, the most effective combination of drugs and miRNAs for mutated cancer therapy design are obtained and these results were validated by the literature. The proposed modelling and analysis approach can be applied to each human disease, starting from the corresponding signalling pathway.

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Prognostic role of circulating tumor cells in patients with EGFR‐mutated or ALK‐rearranged non‐small cell lung cancer

Cited by 20 publications

References 20 publications

Liquid Biopsy for the Detection of Resistance Mechanisms in NSCLC: Comparison of Different Blood Biomarkers

Liquid Biopsy for the Detection of Resistance Mechanisms in NSCLC: Comparison of Different Blood Biomarkers

Ursolic acid suppresses the biological function of osteosarcoma cells

MiRNA therapeutics based on logic circuits of biological pathways

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