Prognostic Role of 14F7 Mab Immunoreactivity against N-Glycolyl GM3 Ganglioside in Colon Cancer

Abstract

Purpose. To assess the prognostic role of 14F7 Mab immunoreactivity, against N-Glycolyl GM3 ganglioside, in patients with colon cancer (CC) and to evaluate the relationship between its expression and clinicopathological features. Methods. Paraffin-embedded specimens were retrospectively collected from 50 patients with CC operated between 2004 and 2008. 14F7 Mab staining was determined by immunohistochemistry technique and its relation with survival and clinicopathologic features was evaluated. Results. The reactivity of 14F7 Mab was detected in all cases. Most cases had high level of immunostaining (70%) that showed statistical correlation with TNM stage (P = 0.025). In univariate survival analysis, level of 14F7 Mab immunoreactivity (P = 0.0078), TNM Stage (P = 0.0007) and lymphovascular invasion (0.027) were significant prognostic factors for overall survival. Among these variables, level of 14F7 Mab immunoreactivity (HR = 0.268; 95% CI 0.078–0.920; P = 0.036) and TNM stage (HR = 0.249; 95% CI 0.066–0.932; P = 0.039) were independent prognostic factors on multivariate analysis. Conclusions. This study is the first approach on the prognostic significance of 14F7 Mab immunoreactivity in patients with colon adenocarcinoma and this assessment might be used in the prognostic estimate of CC, although further studies will be required to validate these findings.

Prognostic Significance of N-Glycolyl GM3 Ganglioside Expression in Non-Small Cell Lung Carcinoma Patients: New Evidences

“…It is the first report describing the relation between the 14F7 Mab reactivity and a more aggressive biological behavior of NSCLC. Comparable results were recently obtained in colon adenocarcinoma by mean of a similar integrated IHC score [ 15 ]. Notably, when only the intensity of reaction or the percentage of 14F7-positive cells was analyzed, in general, no significant associations with clinicopathological features were noted [ 15 ], resembling the present research.…”

“…Comparable results were recently obtained in colon adenocarcinoma by mean of a similar integrated IHC score [ 15 ]. Notably, when only the intensity of reaction or the percentage of 14F7-positive cells was analyzed, in general, no significant associations with clinicopathological features were noted [ 15 ], resembling the present research. In this sense, our data permit us to suggest the use of an IHC score integrating both parameters, although further studies are needed to validate these findings.…”

“…Additionally, the relevance of NeuGcGM3 in cancer progression and its capability of modulating CD4 expression on T cells have been published [ 13 , 14 ]. Recently, the expression of NeuGcGM3, as shown as 14F7 Mab staining, has been associated with more aggressive disease in colon adenocarcinoma [ 15 ]. In NSCLC patients, the expression of NeuGcGM3 inhibits dendritic cells (DCs) differentiation, maturation, and migration leading to tumor-induced DCs suppression [ 9 ].…”

Immunoreactivity of the 14F7 Mab raised against N‐Glycolyl GM3 Ganglioside in retinoblastoma tumours

“…This variant contains glycolylated sialic acid, which is not detected in normal human tissues and fluids, including the healthy retina (Higashi et al 1988;Marquina et al 1996;Scursoni et al 2010Scursoni et al , 2011Blanco et al 2011Blanco et al , 2012Blanco et al , 2013Zhong et al 2012;Lahera et al 2014). Human cells lack of the enzyme cytidine monophospho-N-acetylneuraminic acid hydroxylase, which regulates its synthesis (Irie & Suzuki 1998;Malykh et al 2001).…”

“…The glycolylated variant of the monosialic acid GM3 (NeuGc-GM3) has also been found to be abundantly expressed in many human tumours (Marquina et al 1996;Carr et al 2003;Alfonso et al 2007;Scursoni et al 2010Scursoni et al , 2011Blanco et al 2011Blanco et al , 2012Blanco et al , 2013Zhong et al 2012;Lahera et al 2014). This variant contains glycolylated sialic acid, which is not detected in normal human tissues and fluids, including the healthy retina (Higashi et al 1988;Marquina et al 1996;Scursoni et al 2010Scursoni et al , 2011Blanco et al 2011Blanco et al , 2012Blanco et al , 2013Zhong et al 2012;Lahera et al 2014). Human cells lack of the enzyme cytidine monophospho-N-acetylneuraminic acid hydroxylase, which regulates its synthesis (Irie & Suzuki 1998;Malykh et al 2001).…”