Prognostic relevance of immunophenotyping in 379 patients with acute myeloid leukemia

Chang, Hong; Salma, Fariha; Yi, Qin-long; Patterson, Bruce; Brien, Bill; Minden, Mark D.

doi:10.1016/s0145-2126(03)00180-2

Cited by 115 publications

(97 citation statements)

References 20 publications

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“…Indeed, in non-M3 subtypes, 73.1% of CD34-positive patients had chromosome abnormalities. Although some reports failed to find the correlation between CD19 expression and the presence of t(8;21) in M2 cases (1), our results also further support the well-recognized knowledge that the expressions of CD15, CD19, CD34, and CD56 were significantly increased in AML with t(8;21) (9)(10)(11). Reports showed that immunophenotyping had a sensitivity of 100% and a specificity of 99% for predicting PML/RARalpha gene rearrangements, and the expression pattern of CD34, CD15, and CD13 was highly characteristic of the presence of it (2).…”

Section: Discussionsupporting

confidence: 90%

“…Previous studies suggested that several antigens such as CD34, CD56, and TdT on AML cells were highly related to abnormal chromosome karyotype, but the patients found with cytogenetic changes were mostly lacking special immunophenotypes (9,10). Our study showed that about 54.1% of AML had at least one type of chromosome change.…”

Section: Discussionsupporting

confidence: 44%

“…Cytogenetic changes, age, and WBC counts were currently used as independent prognostic indicators, but it is controversial whether immunophenotype parameters could be further indicators (10,12). We studied the correlations of immunophenotype to clinical features in AML patients.…”

Section: Discussionmentioning

confidence: 99%

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A correlation study of immunophenotypic, cytogenetic, and clinical features of 180 AML patients in China

Zheng

Wang

et al. 2007

Cytometry Part B Clinical

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New WHO classification has been widely applied in the diagnosis of leukemia. To elucidate the immunophenotype of acute myeloid leukemia (AML) and characterize the correlation among morphological, immunological, cytogenetic, and clinical features, we studied the bone marrow immunophenotypes of 180 AML patients in China by flow cytometry. The results showed that CD34, CD2, CD14, CD19, CD56, and HLA-DR were correlated with FAB subtypes. Amongst the 180 patients enrolled in this study, 122 cases were also subjected to karyotype analysis by G-banding technology and abnormal karyotypes were detected in 69 out of 122 patients. Correlation assay showed that t(8;21) was only present in 16 AML-M2 patients, and strongly associated with the individual or combinational expressions of CD15/CD19/CD34/CD56. As to M3, although lymphoid lineage antigens were observed in a considerable number of patients, they were never detected in t(15;17) positive patients. The expressions of CD22, CD56, and TdT showed significant correlation with the overall presence of abnormal karyotype. Additionally, the expressions of CD4, CD7, CD14, CD56, and TdT were positively correlated with clinical features such as white blood cell count, platelet count, and patient's age. In conclusion, immunophenotype analysis was useful for AML diagnosis and classification. At the same time, the data also suggested that the karyotype abnormalities and clinical features were tightly linked with abnormal antigen expression characteristics in AML patients. As one of the largest correlative study performed in China, the results highlighted the importance of a morphological, immunological, and cytogenetic classification of AML that might constitute a working basis for future studies aimed at a better definition of clinicopathological features and optimal treatment strategy for these leukemias. q 2007 Clinical Cytometry Society

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 44%

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A correlation study of immunophenotypic, cytogenetic, and clinical features of 180 AML patients in China

Zheng

Wang

et al. 2007

Cytometry Part B Clinical

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“…In most reports CD34 positivity in non-APL AML has varied between 55.8 and 69.1% [13][14][15]. We have found that HLA-DR is the single best marker for distinguishing APL from other Fig.…”

Section: Leukemia Subtypesmentioning

confidence: 53%

Flowcytometric Immunophenotypic Profile of Acute Leukemia: Mansoura Experience

Salem

El-Aziz

2011

Indian J Hematol Blood Transfus

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Acute leukemia (AL) displays characteristic patterns of antigen expression, which facilitate their identification and proper classification. The purpose of this study is to evaluate the diagnostic usefulness of commonly used immune-markers for immunophenotyping of AL and to define the best immune-markers to be used for proper diagnosis and classification of AL. Besides, to recognize the frequency of different AL subtypes and the antigen expression profile in our Egyptian patients. We retrospectively analyzed the immunophenotypic data of 164 de novo AL patients from our institution during 2009 and 2010. Among these patients, 68.9% were classified as acute myeloblastic leukemia (AML) while 31.1% classified as acute lymphoblastic leukemia (ALL). The commonest FAB subtype in AML group was AML-M4/5 (34.5%) which may differ from most published data. As regard ALL, there were 74.5% with B-ALL and 25.5% with T-ALL. It was found that combined use of HLADR and CD34 was much more helpful in distinguishing APL from non-APL AML than either of these antigens alone. It was found that cCD79a and CD19 were the most sensitive marker for B-ALL while cCD3, CD7 and CD5 were the most sensitive antigens for T-ALL. Our analysis of AL phenotypes proved that employed antibody panels are adequate for proper diagnosis and classification of AL. Flowcytometry was found to be especially useful in the identification of AML-M0 and differentiation of APL from non-APL AML. Immunophenotyping results and FAB classification of our AL patients were comparable to internationally published studies apart from predominance of AML-M4/5 and more frequent APL.

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“…The prognostic significance of the expression of particular antigens remains controversial with previous studies mostly focusing on single antigens [11][12][13][14]. In contrast, the influence of 3 immunophenotypic maturity of AML blasts on overall prognosis is largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Flow cytometric maturity score as a novel prognostic parameter in patients with acute myeloid leukemia

et al. 2015

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Abstract:The European LeukemiaNet (ELN) classification is widely accepted for risk stratification of patients with acute myeloid leukemia (AML). In order to establish immunophenotypic features that predict prognosis, the expression of single AML blast cell antigens has been evaluated with partly conflicting results; however, the influence of immunophenotypic blast maturity is largely unknown.In our study, 300 AML patients diagnosed at our institution between 01/2003 and 04/2012 were analyzed. A flow cytometric maturity score was developed in order to distinguish "mature" AML (AML-ma) from "immature" AML (AML-im) by quantitative expression levels of early progenitor cell antigens (CD34, CD117, and TdT).AML-ma showed significantly longer relapse-free survival (RFS) and overall survival (OS) than AML-im (p<0.001). Interestingly, statistically significant differences in RFS and OS were maintained within the Our novel flow cytometric score easily determines AML blast maturity and can predict clinical outcome. It remains to be clarified whether these results simply reflect an accumulation of favorable molecular phenotypes in the AML-ma subgroup or whether they rely on biological differences such as a higher proportion of leukemia stem cells and/or a higher degree of genetic instability within the AML-im subgroup.

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Prognostic relevance of immunophenotyping in 379 patients with acute myeloid leukemia

Cited by 115 publications

References 20 publications

A correlation study of immunophenotypic, cytogenetic, and clinical features of 180 AML patients in China

A correlation study of immunophenotypic, cytogenetic, and clinical features of 180 AML patients in China

Flowcytometric Immunophenotypic Profile of Acute Leukemia: Mansoura Experience

Flow cytometric maturity score as a novel prognostic parameter in patients with acute myeloid leukemia

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