Prognostic Evaluation of the Expression of p53 and bcl-2 Oncoproteins in Patients with Surgically Resected Non-small Cell Lung Cancer

Lai, Ruay-Sheng; Wang, Jyh-Seng; Hsu, Hon-Ki; Lin, Chin-Hsun; Lin, Min‐Hsi

doi:10.1093/jjco/hyf084

Cited by 20 publications

(11 citation statements)

References 25 publications

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“…The results for expression of Bcl‐2 in AC (2 of 19 samples; 11%) and SCC (8 of 22 samples; 36%) also are in close agreement with the results of Lai et al,5 who reported expression in 15.5% of AC samples and 35% of SCC samples. Although it did not reach significance in the current study ( P = 0.058) (Table 3), the trend toward increased overexpression of Bcl‐2 in SCC samples compared with AC samples was in agreement with previous reports, which showed that expression of Bcl‐2 was associated significantly with this histologic type of NSCLC 8, 10–11.…”

Section: Discussionsupporting

confidence: 90%

“…The overexpression of Bcl‐2 in 10 of 41 samples (24%) is in close agreement with reports published to date. Results of 23%5 and 20%6 have been reported recently in series of 114 and 60 NSCLC samples, respectively. Lai et al5 studied samples of Stage I–IIIa NSCLC, and Tomita et al6 included only later‐stage disease (Stage IIIa–IV).…”

Section: Discussionmentioning

confidence: 79%

“…Results of 23%5 and 20%6 have been reported recently in series of 114 and 60 NSCLC samples, respectively. Lai et al5 studied samples of Stage I–IIIa NSCLC, and Tomita et al6 included only later‐stage disease (Stage IIIa–IV). The similar results reported in those 2 studies suggest that Bcl‐2 expression may be independent of stage in NSCLC.…”

Section: Discussionmentioning

confidence: 79%

“…Discrepancies in the results were resolved by consensus after reassessment of the slide by both investigators. For Bcl‐2 and Bcl‐X L , a section was considered positive if > 10% of tumor cells were stained, because overexpression of these proteins inhibits apoptosis and, thus, is detrimental 5, 6. For Bax, Bak, Bad, Bid, and Bim/Bod, in which the loss of expression is detrimental, sections were scored as negative if expression was lost in > 50% of tumor cells 7.…”

Section: Methodsmentioning

confidence: 99%

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2008

Biopolymers

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 79%

Section: Methodsmentioning

confidence: 99%

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2008

Biopolymers

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“…[42][43][44] Because acrolein mainly excretes through urine, we choose the bladder to investigate CYP-induced toxicity. One is acrolein breaks redox balance and causes oxidative stress in animal tissue; another mechanism is direct attack of ROS.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of lipoic acid and mesna on cyclophosphamide‐induced haemorrhagic cystitis in mice

Song

Liu

et al. 2013

Cell Biochemistry & Function

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The protective roles of lipoic acid (LA)/vitamin C (VC) and mesna on preventing cyclophosphamide (CYP)-induced haemorrhagic cystitis (HC) were investigated. Swiss mice were divided into five groups randomly. HC was induced by a single dose of CYP injection (150-mg kg(-1) bodyweight). Group I was injected with saline (four times in total) throughout as control group. Group II received CYP and three equal doses of saline. Group III received CYP and three doses of mesna, whereas Group IV (or Group V) received CYP, mesna + two doses of VC (or LA). All injections were performed intraperitoneally. After 24 h of cystitis induction, the bladders were collected for all the experiments. Histological characterization showed that CYP injection resulted in severe HC. Reactive oxygen species (ROS) and thiobarbituric acid reactive substances' levels were increased in CYP group. The activities of antioxidant enzymes, e.g. superoxide dismutase, catalase, glutathione S-transferase and glutathione peroxidase, were inhibited significantly in CYP groups, respectively. In addition, activation of c-jun N-terminal kinases (JNK) and p38 mitogen-activated protein kinase (MAPK) may be involved in the mechanism of CYP-induced HC but not extracellular signal regulated kinases (ERK). Significant suppression of p38 phosphorylation on Group V suggests that LA and mesna may have synergistic beneficial effect. In Groups III-V, all the parameters of HC and oxidative stress were inhibited significantly. Taking together, we found that these results illustrated that ROS play an important role on CYP-induced HC and the administration of LA/VC with mesna may have therapeutic potential against CYP-induced bladder HC.

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The expression of Bcl‐2 family proteins differs between nonsmall cell lung carcinoma subtypes

Berrieman

Smith

O’Kane

et al. 2005

Cancer

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BACKGROUNDProteins of the Bcl‐2 family play a key role in the control of apoptosis and carry out both proapoptotic and antiapoptotic functions. However, with the exception of Bcl‐2 itself, little is known about the expression of these potentially critical proteins in nonsmall cell lung carcinoma.METHODSImmunohistochemistry was used to study the expression of Bcl‐2 and 6 other Bcl‐2 family proteins in a pilot series of 41 archival nonsmall cell lung carcinoma specimens (19 adenocarcinomas and 22 squamous cell carcinomas).RESULTSOverexpression of the apoptosis inhibitors Bcl‐2 and Bcl‐XL was observed in 10 of 41 samples (24%) and in 11 of 41 samples (27%), respectively. Loss of expression of proapoptotic proteins was observed as follows: Bak, 24 of 41 samples (59%); Bad, 21 of 41 samples (51%); Bid, 20 of 41 samples (49%); Bax, 14 of 41 samples (34%); and Bim/Bod, 2 of 41 samples (5%). Statistically significant differences in expression between adenocarcinoma samples and squamous cell carcinoma samples were observed for Bcl‐XL (overexpression in 11 of 19 adenocarcinomas [58%] vs. 0 of 22 squamous cell carcinomas [0%]; P < 0.001) and for Bad (loss of expression in 5 of 19 adenocarcinomas [26%] vs. 16 of 22 squamous cell carcinomas [73%]; P = 0.004).CONCLUSIONSAlthough this was only a pilot study, the results revealed significant differences in the expression of apoptosis‐related proteins both between individual samples of nonsmall cell lung carcinoma and between the two main histologic subtypes. Such differences may play a role in the development of lung tumors; and, if it is found that these differences are of clinical importance, then it may be required to regard nonsmall cell lung carcinoma subtypes as separate entities rather than as one disease. Cancer 2005. © 2005 American Cancer Society.

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Prognostic Evaluation of the Expression of p53 and bcl-2 Oncoproteins in Patients with Surgically Resected Non-small Cell Lung Cancer

Abstract: Expressions of bcl-2 and p53 using immunohistochemical staining are not independent prognostic predictors in patients undergoing surgery for NSCLC.

Cited by 20 publications

References 25 publications

Research highlights

Research highlights

Protective effects of lipoic acid and mesna on cyclophosphamide‐induced haemorrhagic cystitis in mice

The expression of Bcl‐2 family proteins differs between nonsmall cell lung carcinoma subtypes

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