Prognostic evaluation of Nanog, Oct4, Sox2, PCNA, Ki67 and E-cadherin expression in gastric cancer

Li, Ning; Deng, Wenying; Ma, Jie; Wei, Bing; Guo, Kang; Shen, Wei; Zhang, Yanping; Luo, Suxia

doi:10.1007/s12032-014-0433-6

Cited by 83 publications

(75 citation statements)

References 28 publications

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“…The promoting effect of Oct4 on migration and invasion has also been observed in numerous types of cancer. In gastric cancer, only positive expression of Oct4, but not other proteins, including NANOG, Sox2, proliferating cell nuclear antigen, Ki67 and epithelial cadherin was reported to be correlated with lymphatic invasion (25). Oct4 was also reported to enhance lung cancer cell invasion and adhesion accompanied by the downregulation of epithelial marker cytokeratin and upregulation of the mesenchymal markers vimentin and neural cadherin (26).…”

Section: A B C D Discussionmentioning

confidence: 99%

Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347

et al. 2016

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Abstract. Osteosarcoma is the most common primary bone tumor in children and adolescents, typically presenting with a poor prognosis. Octamer-binding transcription factor 4 (Oct4) protein, encoded by the POU class 5 homeobox 1 gene, is important in maintaining self-renewal of pluripotent stem cells, and is closely associated with cancer. However, its role in osteosarcoma remains to be elucidated. The present study observed Oct4 was markedly increased in osteosarcoma cell lines and in human osteosarcoma tissue samples. Following Oct4 downregulation by small interfering RNA (siRNA) in osteosarcoma F5M2 cells, the cells exhibited significant decreases in proliferation and invasion ability, and an increase in cell apoptosis. Notably, downregulation of Oct4 decreased the expression of AK055347, a newly identified long noncoding RNA (lncRNA) in human tissues. The downregulation of AK055347 by siRNA resulted in a significant suppressive effect on proliferative and invasive ability, and promotion of cell apoptosis in osteosarcoma cells. Thus, the current study suggests Oct4 exerts a promoting effect in osteosarcoma, and identifies a novel lncRNA in osteosarcoma progression.

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Section: A B C D Discussionmentioning

confidence: 99%

Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347

et al. 2016

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“…NM23, a metastasis suppressor protein, is associated with tumor metastasis, while its expression and prognostic value in GC is still controversial 29. As a common marker for proliferation of tumor cells, Ki‐67 is mainly synthesized in the initial stages of cell proliferation and is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent in quiescent cells (G0) 30. E‐cadherin is a Ca 2+ ‐dependent transmembrane glycoprotein, which has influence on cell‐cell adhesion and the suppression of E‐cadherin function and/or expression is closely related to an EMT phenotype 31.…”

Section: Discussionmentioning

confidence: 99%

Correlation between negative expression of pepsinogen C and a series of phenotypic markers of gastric cancer in different gastric diseases

et al. 2018

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Gastric tumorigenesis is a multistep process initiated by chronic superficial gastritis (SG), followed by atrophic gastritis (AG), then intestinal metaplasia (IM), and finally by dysplasia and adenocarcinoma according to the Correa model. Pepsinogen C (PGC) decreases gradually during progression of cancer, which makes PGC an ideal negative marker for GC. To explore the correlation between PGC and other positive tumor markers in different gastric diseases, we observed the expression of PGC, MG7‐Ag, MMP9, NM23, Ki‐67, and E‐cadherin by immunohistochemistry, quantitative RT‐PCR, and immunoblot analysis. Our results showed that in SG, PGC was highly expressed while malignant phenotype markers were rarely expressed. In contrast with SG, malignant phenotype markers were highly expressed while the positive rate of PGC reached only 1.44% in GC. So there was no coexpression of PGC and malignant phenotype markers in SG or GC tissues. Only in the AG group, which is well‐known to be gastric precancerous disease, coexpression of PGC and malignant phenotype markers was detected. Our results suggested that the expression of PGC in AG was negatively correlated with that of MG7‐Ag and MMP9. Of all AG, those with low expression of PGC and high expression of MG7‐Ag and MMP9 may possess a greater potential of malignant transformation. Combined detection of negative marker PGC and positive markers MG7‐Ag and MMP9 could be used as a potential follow‐up panel for monitoring dynamical progression of AG and improving the detection efficiency of high‐risk individuals of gastric cancer, and then taking necessary interventions on the target population.

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“…These pluripotency-inducing factors have been detected in embryonic stem cells, as well as in normal cells and cancer cells, including gastric cancer (9). Although these factors are necessary in order for stem cells to acquire pluripotency, it has been suggested that they may have oncogenic potential in normal cells (10).…”

Section: Introductionmentioning

confidence: 99%

Prognostic significance of KLF4 expression in gastric cancer

et al. 2016

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Abstract.To understand the roles of pluripotent stem cell-inducing genes in gastric cancer, the expression of Krüppel-like factor 4 (KLF4), Nanog, octamer-binding transcription factor 4 (Oct4), avian myelocytomatosis viral oncogene homolog (c-Myc) and sex-determining region Y-box 2 (SOX2) was examined using the newly developed gastric carcinoma tissue microarray. The associations between the immunohistochemical expression levels of the pluripotency-inducing factors and the clinicopathological data of 108 patients with gastric cancer were analyzed. No associations were identified between the expression levels of the five pluripotency-inducing factors and the tumor-node-metastasis (TNM) classification or clinicopathological characteristics of the patients. In addition, multivariate analysis revealed no association of Nanog, Oct4, SOX2 or c-Myc with the prognosis of the gastric cancer patients; however, low expression of KLF4 was determined to be an independent negative prognostic factor (P=0.0331), particularly in patients who underwent R0 resection (TNM stages 2 and 3; P=0.0048). In summary, low KLF4 expression was found to be negatively associated with overall survival, and may therefore be a useful prognostic marker in gastric cancer patients. IntroductionGastric cancer is a malignant tumor with a poor prognosis, and the various treatments available at present, including surgery, chemotherapy and radiotherapy, remain unsatisfactory (1). Development of gastric cancer is associated with a number of molecular abnormalities, the majority of which affect the downstream signal transduction pathways involved in cell growth and differentiation (2,3). Investigation into such molecules is expected to provide useful prognostic biomarkers of gastric cancer that will aid in determining the treatment plan for individual patients. For example, methylation of the XIAP-associated factor 1 promoter (4), and expression of 14-3-3σ (5) and miR-200c (6) have been reported to be important for the prediction of poor prognosis in gastric cancer patients; however, further investigation is required.Induced pluripotent stem (iPS) cells are cells that have acquired pluripotency following the introduction of various factors; octamer-binding transcription factor (Oct)3/4 and sex-determining region Y-box 2 (SOX2) plus Krüppel-like factor 4 (KLF4) and avian myelocytomatosis viral oncogene homolog (c-Myc), or Nanog and LIN28 have been shown to induce pluripotency in various murine and human cells (e.g., fibroblasts) (7,8). These pluripotency-inducing factors have been detected in embryonic stem cells, as well as in normal cells and cancer cells, including gastric cancer (9). Although these factors are necessary in order for stem cells to acquire pluripotency, it has been suggested that they may have oncogenic potential in normal cells (10).Expression of SOX2 has been reported to be important for tumorigenicity and chemoresistance (11). However, the inhibition of gastric cancer cell growth and the induction of apoptosis by SOX2 has also been rep...

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Prognostic evaluation of Nanog, Oct4, Sox2, PCNA, Ki67 and E-cadherin expression in gastric cancer

Cited by 83 publications

References 28 publications

Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347

Transcription factor Oct4 promotes osteosarcoma by regulating lncRNA AK055347

Correlation between negative expression of pepsinogen C and a series of phenotypic markers of gastric cancer in different gastric diseases

Prognostic significance of KLF4 expression in gastric cancer

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