Prognostic Biomarkers Based On GUF1, EFTUD2 and GSPT1 Targets Affecting Migration of Gastric Cancer Cells.

Abstract

Eukaryotic Elongation Factor 1 Alpha 2 (EEF1A2) is a protein coding gene which involved in tumor development and progression in several types of human cancer, but little is known about the function of EEF1A2 proteins in gastric cancer (GC).The ONCOMINE and the TCGA databases were used to evaluate the expression of GUF1, EFTUD2, GSPT1 and GSPT2 in GC and the association of EEF1A2 family with individual clinical characteristics. Kaplan-Meier (K-M) Plotter hint the prognostic value of GUF1, EFTUD2, GSPT1 and GSPT2. GSE62254 and GSE66222 dataset were used to validated the expression of GUF1, EFTUD2, GSPT1. Human AGS and GES cells lines were also used for validating the function of GUF1, EFTUD2, GSPT1. RNA interference (RNAi) of GUF1, EFTUD2 and GSPT1 have been used to query those genes expression pattern and dissect the proliferation and migration in GC cells line. The results showed that GUF1, EFTUD2 and GSPT1 were significantly up-regulated in GC cell lines. High expression of GUF1, EFTUD2 and GSPT1 was correlated with cell proliferation and migration induced in GC cells. We report GUF1, EFTUD2 and GSPT1 may be potential novel oncogenes that helps to maintain the survival of GC cells. Our study identified that high level of GUF1, EFTUD2 and GSPT1 expression are predictive biomarkers for a poor prognosis in GC.