Prognosis of breast-carcinoma lymphagenesis evaluated by immunohistochemical investigation of vascular-endothelial-growth-factor receptor 3

Vascular endothelial growth factor-D is an independent prognostic factor in epithelial ovarian carcinoma

“…In the present study, we found a significant correlation between VEGFR-3 expression and lymph node metastasis (Table 2). However, we did not find that outcome was predicted by the presence of VEGFR-3 in endothelial cells adjacent to tumour nests (Table 3), which is consistent with similar studies in breast, nonsmall cell lung and colorectal carcinoma (Jacquemier et al, 2000;Kajita et al, 2001;White et al, 2002).…”

“…While VEGFR-3 protein levels were not related to lymphatic spread in gastric and breast carcinoma (Yonemura et al, 1999;Jacquemier et al, 2000), VEGFR-3 detection was found to predict the presence of lymphatic metastases in oral squamous cell carcinoma (Moriyama et al, 1997). In the present study, we found a significant correlation between VEGFR-3 expression and lymph node metastasis (Table 2).…”

The Akt expression correlates with the VEGF-A and -C expression as well as the microvessel and lymphatic vessel density in breast cancer

“…A significant correlation was also found between LVD and the regional lymph node status, in accordance with previous studies on breast cancer (37,38,44,46), while no significant correlation was found in a series of 60 breast cancers (47). A significant correlation between LVD and the regional lymph nodes was also found in head and neck (48,49), lung (43), thyroid (50) and gastric (51) cancers.…”

“…On the other hand, VEGF-C was demonstrated to induce angiogenesis in the mouse cornea (66). Although VEGF-A and VEGF-C have been shown to play various roles in both angiogenesis and lymphangiogenesis in the animal models, there have been conflicting reports regarding the relationship between VEGF-A and VEGF-C and the LVD and MVD in human cancer tissues (37,41,45,47,55,(67)(68)(69)(70). The VEGF-C expression correlated positively with both the LVD and MVD in bladder (67), gastric (41) and ovarian (45) cancers, while no significant correlation ONCOLOGY REPORTS 23: 621-630, 2010 Table VII.…”

Human chondrosarcoma secretes vascular endothelial growth factor to induce tumor angiogenesis and stores basic fibroblast growth factor for regulation of its own growth

“…The final quantification was carried out on the number of microvessels per mm 2 obtained from the 4 fields at a magnification of ϫ200, as described. 33,34 Cell proliferation assays Cultured HUVECs were washed with PBS and dispersed in a 0.25% trypsin, 1 mM EDTA solution. A cell suspension was made in M200S (LSGSϩ), plated as 1 ϫ 10 4 cells/well onto 96-well plastic tissue culture plates (Coster, Cambridge, MA) and incubated (37°C, 5% CO 2 ) for 24 hr.…”