Prognosis after Surgical Excision of Canine Melanomas

Bostock, D. E.

doi:10.1177/030098587901600103

Cited by 134 publications

(231 citation statements)

References 2 publications

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“…This finding is in stark contrast to previous studies, which demonstrated prognostic value associated with histologic grading. 4,5,30,31 While the results of the present study are intriguing, it is difficult to draw any conclusions from that information as this was not the focus of the present study and confounding variables are almost certainly present.…”

Section: Discussionmentioning

confidence: 68%

“…15,16 These mutations allow autophosphorylation of the receptor, independent of ligand interaction, and result in unregulated * HI 5 greater than 80% immunoreactivity (n 5 4); N 5 no immunoreactivity (n 5 34); LI 5 less than 10% immunoreactivity (n 5 8); AI 5 all immunoreactivity (n 5 12). Vet Pathol 46: 5,2009 activation. 16,23,28 Tyrosine kinase inhibitors are a targeted therapy that is being successfully utilized for GIST in people and is in clinical trials for canine MCT.…”

Section: Discussionmentioning

confidence: 99%

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Immunohistochemical Expression of c-KIT Protein in Feline Soft Tissue Fibrosarcomas

2009

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Abstract. C-KIT is the cellular homolog of the feline sarcoma viral oncogene v-KIT, which encodes the tyrosine kinase receptor protein KIT. Mutations and varied expression of this gene have been demonstrated within multiple neoplasms in people and domestic animals. The purpose of this study was to determine if KIT protein is expressed in feline soft tissue fibrosarcomas (ST FSA) using immunohistochemistry (IHC). The computer database at the Oklahoma Animal Disease Diagnostic Laboratory was searched from January 1, 2006, to December 31, 2007, for any domestic cat with an ST FSA. Routinely stained slides from 46 feline ST FSAs were reviewed and graded based on the scale outlined by Kuntz et al. Immunohistochemistry for KIT protein was performed on one representative section from each cat. There were a total of 12/46 (26%) cats that were immunoreactive for KIT. Immunoreactivity was detected in greater than 80% of the neoplastic cells in 4/46 (9%) cats. Immunoreactivity was detected in less than 10% of the neoplastic cells in 8/46 (17%) cats. Immunoreactivity was characterized by evenly distributed cytoplasmic stippling within the neoplastic spindle-shaped cells and/or multinucleated giant cells. Based on these results, KIT immunoreactivity can be detected within feline ST FSAs using IHC. The results of this study also indicate that KIT immunoreactivity in feline ST FSA does not correlate with the histologic grade (P 5 .141, X 2 5 2.166), survivability (P 5 .241, X 2 5 1.373), or whether the neoplasm was a spontaneous or an injection site FSA (P 5 .074, X 2 5 3.184).

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Expression of c-KIT Protein in Feline Soft Tissue Fibrosarcomas

2009

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“…After histological diagnosis of fibrosarcoma, haemangiopericytoma, myxosarcoma or neurofibrosarcoma, all dogs that showed no clinical evidence of residual tumour or metastatic lesions immediately after surgery were followed until death or for at least three years. Post-surgical studies and statistical procedures were as previously described for dogs with melanoma [2].…”

Section: Methodsmentioning

confidence: 99%

Prognosis after Surgical Excision of Canine Fibrous Connective Tissue Sarcomas

1980

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Abstract. One hundred eighty seven dogs from which fibrous connective tissue sarcomas had been excised were studied until death or for at least 3 years after surgery. Dogs with a skin fibrosarcoma had a median survival time of 80 weeks, compared with 140 weeks for animals with haemangiopericytoma in similar sites, this difference being statistically significant. However, the difference in survival time between the two histologic types disappeared when tumours with a similar mitotic index were compared. Dogs with a tumour of mitotic index 9 or more had a median survival time of 49 weeks, compared with 118 weeks for those with a tumour of mitotic index less than 9, regardless of tumour morphology. Tumour recurrence rates of 62% and 25% respectively for the two groups were also significantly different.Fibrous connective tissue sarcomas including fibrosarcomas, haemangiopericytomas, neurofibrosarcomas and myxosarcomas comprise between 9% and 14% of all canine skin neoplasms [ 1,3], and fibrosarcoma is the second most common intra-oral malignancy in dogs [4]. There are few accounts, however, of the behaviour of these tumours following therapy, and especially surgery. Some well differentiated skin fibrosarcomas were reported to be locally invasive and, rarely, to metastasize [3], but too few cases have been followed to allow definite conclusions concerning their degree of malignancy [9].Recurrence rates after surgery for canine haemangiopericytoma have varied from 7% [6] to 56% [lo]. Most authors agree on the relative infrequency of metastasis, but behaviourally benign and malignant neoplasms have been reported to show little histological difference [lo]. Accurate prognosis, to identify dogs requiring postsurgical therapy, is best reached through knowledge of the probability of recurrence or metastasis of various morphological groups of tumours following apparently complete surgical excision. Materials and MethodsSurgically excised tumour specimens fued in formol saline were received over a four-year period beginning in January 1970, from 19 veterinary practices in southeast England. Accompanying information recorded the age, sex and breed of dog, the size of the mass and the 581

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“…52,53 Mucosal melanomas are highly metastatic and have a poor prognosis in the higher grades and stages. 54,55 As is the case for advanced melanoma in humans, melanoma in dogs is generally resistant to chemotherapy and radiation therapy, and metastasis is the major cause of treatment failure and death. 56 -58 Finally, the dog offers a good translational model because of its large size, spontaneous development of tumors, rapid assessment of therapeutic activity, and biological similarity to humans.…”

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confidence: 99%

Preclinical development of human granulocyte-macrophage colony-stimulating factor-transfected melanoma cell vaccine using established canine cell lines and normal dogs

Jk²,

Culp³

et al. 1999

Cancer Gene Ther

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Tumor vaccines and gene therapy have received significant attention as means of increasing cellular and humoral immune responses to cancer. We conducted a pilot study of seven research dogs to determine whether intradermal injection of canine tumor cells transfected via the Accell particle-mediated gene transfer device with the cDNA for human granulocyte-macrophage colony-stimulating factor (hGM-CSF) would generate biologically relevant levels of protein and result in demonstrable histological changes at sites of vaccination. Tumor cell vaccines of 10 7 irradiated canine melanoma cells were nontoxic, safe, and well tolerated. No significant alterations in blood chemistry values or hematological profiles were detected. A histological review of control vaccine sites revealed inflammatory responses predominated by eosinophils, whereas vaccine sites with hGM-CSF-transfected tumor cells had an influx of neutrophils and macrophages. Enzyme-linked immunosorbent assays of skin biopsies from vaccine sites had local hGM-CSF production (8.68 -16.82 ng/site of injection) at 24 hours after injection and detectable levels (0.014 -0.081 ng/site) for Յ2 weeks following vaccination. Flow cytometric analysis of hGM-CSF-transfected cells demonstrated Յ25% transfection efficiency, and hGM-CSF levels obtained during time-course assays demonstrated biologically relevant levels for both irradiated and nonirradiated samples. These data demonstrate the in vivo biological activity of irradiated hGM-CSF-transfected canine tumor cells and help provide evidence for a valid translational research model of spontaneous tumors.

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Prognosis after Surgical Excision of Canine Melanomas

Cited by 134 publications

References 2 publications

Immunohistochemical Expression of c-KIT Protein in Feline Soft Tissue Fibrosarcomas

Immunohistochemical Expression of c-KIT Protein in Feline Soft Tissue Fibrosarcomas

Prognosis after Surgical Excision of Canine Fibrous Connective Tissue Sarcomas

Preclinical development of human granulocyte-macrophage colony-stimulating factor-transfected melanoma cell vaccine using established canine cell lines and normal dogs

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