Progestin Levels in Jugular and Ovarian Venous Blood, Corpora Lutea, and Ovaries of the Nonpregnant Bovine

Gomes, W. R.; Estergreen, V.L.; Frost, O; Erb, R.E.

doi:10.3168/jds.s0022-0302(63)89094-3

Cited by 41 publications

(16 citation statements)

References 22 publications

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“…Compared to previous reports (Mares et al 1962;Gomes et al 1963;Hafs 8c Armstrong 1968;Veenhuizen et al 1972;Gomes 8c Erb 1965;Erb et al 1971), the progesterone concentration found in the present investigation was low during most stages of the cycle. The concentration of 20/?-hydroxy-4-pregnen-3-one was about the same as those found by other investigators.…”

Section: Concentrations Of Steroids In Bovine Corpus Luteumcontrasting

confidence: 80%

Identification and Quantitative Determination of Steroids in Bovine Corpus Luteum During Oestrous Cycle and Pregnancy

Axelson

Schumacher

Sjövall

et al. 1975

Acta Endocrinologica

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Neutral steroids in bovine corpus luteum were isolated by liquid-gel chromatography on hydrophobic Sephadex, and were analyzed by computerized gas chromatography-mass spectrometry. The presence of progesterone and 2 0 \ g = b \ -h y d r o x y -4 -p r e g n e n -3 -o n e was confirmed. In addition, 3\g=b\-hydroxy-5-pregnen -20-one, 5-pregnene-3 \ g = b \ , 2 0 \ g = b \ -diol, 3\g=b\-hydroxy-5\g=a\\x=req-\ pregnan-20-one and 5 \ g -a \ -p r e g n a n e -3 \ g = b \ , 2 0 \ g = b \ -d i o l , 3 2 were fully identified, and 3-hydroxy-4-pregnen-20-onne, 4 pregnene-3,20-diol, 22-hydroxycholesterol and 20,22-dihydroxycholesterol were partially characterized. Steroid sulphates were not detected. Quantification of the six fully identified steroids was based on peak areas in specific fragment ion current chromatograms constructed by the computer. During the 4th\p=n-\19th day of the oestrous cycle the steroid concentrations varied as follows: progesterone 6.0\p=n-\36.7 \g=m\g/g wet luteal weight, 20\g=b\-hydroxy-4-pregnen-3-one 0.8\p=n-\5.5 \g=m\g/g, 3 \ g = b \ -h y d r o x y -5 -p r e g n e n -2 0 -o n e 1.0\p=n-\7.1 \g=m\g/g,5-pregnene-3\g=b\,20\g=b\-diol < 0.2\p=n-\0.9 \g=m\g/g,3\g=b\-hydroxy-5\g=a\-pregnan-20-one 1.7\p=n-\8.6 \g=m\g/g, and 5\g=a\-pregnane-3\g=b\,20\g=b\-diol < 0.2-1.2 \g=m\g/g, The concentrations of progesterone and 3 \ g = b \ -h y d r o x y -5 -p r e g n e n -2 0 -o n e seemed to vary in parallel and were low during days 11\p=n-\17. During this period the concentrations of 5 -p r e g n e n e -3 \ g = b \ , 2 0 \ g = b \ -d i o l and 5\g=a\-pregnane\x=req-\ 3\g=b\,20\g=b\-diol were highest as was the relative contribution of all three 20\ g=b\ \ x=r eq-\ hydroxysteroids to the total amount of steroids. The relative amount of 3 \ g = b \ -h y d r o x y -5 \ g = a \ -p r e g n a n -2 0 -o n e seemed to be highest during days 4\p=n-\6. The total steroid concentration in corpora lutea taken in early pregnancy

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Section: Concentrations Of Steroids In Bovine Corpus Luteumcontrasting

confidence: 80%

Identification and Quantitative Determination of Steroids in Bovine Corpus Luteum During Oestrous Cycle and Pregnancy

Axelson

Schumacher

Sjövall

et al. 1975

Acta Endocrinologica

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“…The increase in progesterone concentrations was observed to be maximum on day 13 in buffaloes. Gomes et al (1963) reported that the highest concentrations of progesterone in luteal tissues in cows and the greatest luteal weight were found on day 13 after ovulation. The timing of these events coincides with plasma progesterone levels in the buffalo.…”

Section: Discussionmentioning

confidence: 97%

Pattern of plasma progesterone, oestradiol-17β, luteinizing hormone and androgen in non-pregnant buffalo (Bubalus bubalis)

Arora

Pandey

1982

Acta Endocrinologica

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Domestic buffaloes were used to characterize the pattern of progesterone, oestradiol-17\g=b\, LH and androgen in the systemic circulation following infertile insemination. Concentrations of hormones were measured by RIA in blood samples collected daily or at alternate days following insemination. The concentration of progesterone was lowest on the day of insemination, and increased significantly to a peak level of 4.00 \m=+-\0.60 ng/ml by day 13 post insemination. After day 17, it declined significantly (P < 0.01) to reach low levels by day 21. The concentration of oestradiol-17\g=b\ was high at the time of insemination and declined significantly (P < 0.01) by day 2 after insemination. It was maintained around the basal level till day 18 with minor peaks in between this period. It again rose significantly (P < 0.01) at subsequent oestrus. The mean level of LH was highest at the time of insemination, and declined significantly (P < 0.01) by day 1 post insemination. It did not vary appreciably till the animal returned to oestrus. The oestrous value of LH and progesterone were negatively correlated (r = \m=-\0.77).The androgen level was observed to be high at insemination in 3 out of 5 animals, but the overall pattern of this steroid was inconsistent during the period studied. A high concentration of androgen was recorded in all the animals from day 2\p=n-\5 before the onset of oestrus.In recent years, it has become generally accepted that secretion of LH during the ovarian cycle of numerous mammalian species is modulated by oestradiol which acts upon two functionally inde¬ pendent feedback systems; a negative feedback system controlling tonic LH secretion and a positive feedback system governing the pre-ovulatory LH surge (Knobil 1974; Brown et al. 1972; Rad¬ ford et al. 1969). Perhaps the only generally ac¬ cepted role for progesterone in the physiological control of LH secretion is to 'block' the LH surge. Consequently, a rise in circulating oestradiol can¬ not elicit a LH surge when a functional corpus luteum is present (Knobil 1974). Information on the hormonal factors responsible for oestrus and ovulation in buffaloes is scarce.Progesterone and oestradiol changes during the oestrous cycle have been reported , while no information is available on the sequence of coincident changes in the plasma con¬ centration of progesterone, oestradiol-17ß, LH and androgen in this species.Buffaloes have a poor conception rate and exhi¬ bit weak symptoms of oestrus (Johari 1960). This reduced fertility is of great economic loss because the buffalo contributes greatly towards milk and meat production in many countries. Therefore, a comprehensive knowledge of the reproductive hormones may contribute towards understanding the mechanism of reproduction control in this species. With this background, the present investi¬ gation was conducted to elucidate the precise tem¬ poral relationship of progesterone, oestradiol-I7ß, LH and androgen in systemic plasma from nonpregnant buffaloes. Such studies may help to estab¬ lish physiologica...

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“…The view that peripheral plasma progesterone levels in the cow bear no consistent relationship to the oestrous cycle (Gomes, Estergreen, Frost & Erb, 1963) can certainly no longer be maintained.…”

Section: Discussionmentioning

confidence: 99%

Competitive Protein-Binding Assay of Progesterone in Bovine Jugular Venous Plasma During the Oestrous Cycle

Shemesh¹,

Lindner²,

Ayalon³

1971

Reproduction

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Summary. A rapid radiometric assay based on thin-layer-chromatography and the competition by progesterone for binding sites on the corticosterone-binding globulin of human plasma was used to determine changes in the level of progesterone in peripheral bovine plasma during the oestrous cycle. The blood level (ng/ml, mean \ m=+-\ S.E.) of the hormone was minimal ( 0\ m=. \ 6\ m=+-\ 0\m=.\15to 0\ m=. \ 8\ m=+-\ 0\m=.\20) from Day \m=-\2 to +2 of the cycle (Day 0 = day of oestrus), rose steeply between Day 3 and Day 7, and either continued to rise very slowly or maintained a plateau fluctuating about a mean of 5\ m=. \ 4\ m=+-\ 0\m=.\16 for 8 to 10 days, before declining; the most abrupt fall in plasma progesterone concentration occurred on Day \m=-\4 or \m=-\3. The results agreed well with control data obtained by sequential paper-and gas-chromatography (r = 0\m=.\93).

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Progestin Levels in Jugular and Ovarian Venous Blood, Corpora Lutea, and Ovaries of the Nonpregnant Bovine

Cited by 41 publications

References 22 publications

Identification and Quantitative Determination of Steroids in Bovine Corpus Luteum During Oestrous Cycle and Pregnancy

Identification and Quantitative Determination of Steroids in Bovine Corpus Luteum During Oestrous Cycle and Pregnancy

Pattern of plasma progesterone, oestradiol-17β, luteinizing hormone and androgen in non-pregnant buffalo (Bubalus bubalis)

Competitive Protein-Binding Assay of Progesterone in Bovine Jugular Venous Plasma During the Oestrous Cycle

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