Progestin inhibits and thrombin stimulates the plasminogen activator/inhibitor system in term decidual stromal cells: implications for parturition

Norwitz, Errol R.; Snegovskikh, Victoria; Schatz, Frederick; Foyouzi, Nastaran; Rahman, Mizanur; Buchwalder, Lynn; Lee, Hee Joong; Funai, Edmund F.; Buhimschi, Catalin S.; Buhimschi, Irina A.; Lockwood, Charles J.

doi:10.1016/j.ajog.2007.02.035

Cited by 20 publications

(22 citation statements)

References 17 publications

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“…GnRH-I and -II also did not alter the production of uPA and PAI-1 by term DSCs, although, consistent with earlier reports (24), progesterone signficantly decreased uPA and increased PAI-1 production in these cells (Fig. 4G-H The increase in PAI-1 but not uPA production by term DSCs in response to thombin is consistent with earlier reports (24). Moreover, in contrast to their effect on first-trimester DSCs but consistent with earlier reports (30), thrombin did not significantly up-regulate sFlt-1 production by term DSCs (Fig.…”

Section: Functional Effects Of Gnrh-i and -Ii On Dscssupporting

confidence: 93%

“…In contrast with earlier publications (9, 54), we were unable to demonstrate any change in uPA and PAI-1 expression with GnRH stimulation. The difference between these publications and the present data may be related to the cell culture methodology, because we have previously shown (24) and confirmed again in the present work (Figs. 3G, 3H, 4G, and 4H) that steroid hormones (specifically progesterone) significantly influence the production of uPA and PAI-1.…”

Section: Figuresupporting

confidence: 75%

“…First-strand complementary DNA (cDNA) was generated by reverse transcription (RT) of 1 mg RNA using p(dT) [12][13][14][15][16][17][18] primers with MMLV-reverse transcriptase (Invitrogen) and amplified as previously described (23,24). The polymerase chain reaction (PCR) products were separated by gel electrophoresis in 1.5% agarose and visualized by ethidium bromide staining on an ultraviolet transilluminator.…”

Section: Reverse-transcription Polymerase Chain Reactionmentioning

confidence: 99%

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Role of GnRH–GnRH receptor signaling at the maternal-fetal interface

Lee

Snegovskikh

Park

et al. 2010

Fertility and Sterility

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Section: Functional Effects Of Gnrh-i and -Ii On Dscssupporting

confidence: 93%

Section: Figuresupporting

confidence: 75%

Section: Reverse-transcription Polymerase Chain Reactionmentioning

confidence: 99%

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Role of GnRH–GnRH receptor signaling at the maternal-fetal interface

Lee

Snegovskikh

Park

et al. 2010

Fertility and Sterility

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“…This is potentially significant given the association of the plasminogen system with the biological changes that accompany parturition (Norwitz et al, 2007). Also of interest was the LPS-responsive increase in the expression of the endothelin 1 gene (EDN1) observed after 2 h exposure.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of the transcriptional response of human decidual cells to lipopolysaccharide stimulation

Himes¹,

Handley²,

Chu³

et al. 2012

Journal of Reproductive Immunology

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Decidual cells are central to innate immunity at the maternal/fetal interface. We sought to characterize the response of decidual cells to stimulation and then removal of lipopolysaccharide (LPS) using a whole genome approach. Decidual cells were isolated from term unlabored cesarean sections. Cells were stimulated with LPS and RNA isolated both prestimulation and 2 and 24 h post-stimulation. Media were changed and RNA extracted 48 h later. Gene expression was measured using Agilent 44K whole genome microarrays. Data were visualized and interpreted using Ingenuity Pathway Analysis (IPA) software and selected (n = 5) target gene expression was verified with quantitative real-time PCR. Genes related to immune function were up-regulated at 2 and 24 h after LPS exposure and then generally returned to baseline or were at least substantially reduced after LPS removal. Pathway analysis also revealed that genes involved in lipid metabolism (specifically cholesterol and steroid biosynthesis), iron metabolism, and the plasminogen system were coordinately altered following exposure to LPS. Our novel, preliminary findings provide insight into possible mechanisms via which the host inflammatory response could contribute to preterm birth and warrant further investigation in preterm samples.

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“…Progesterone activates the specificity protein transcription factor 1 (SP-1) which in turn enhances the transcription of tissue factor (TF), a hemostatic agent and plasminogen activator inhibitor-1 (PAI-1) that inhibits the fibrinolytic pathway (Norwitz et al, 2007). TF inhibits urokinase-type plasminogen activator (uPA) which might participate in activation of TGFβ.…”

Section: Regulation Of Trophoblast Invasion and Migrationmentioning

confidence: 99%

The role of progesterone in implantation and trophoblast invasion

Halász

Szekeres-Barthó

2013

Journal of Reproductive Immunology

103

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Publication informationJournal of Reproductive Immunology, 97 (1): 43-50Publisher Elsevier Item record/more information http://hdl.handle.net/10197/5006 Publisher's statementThis is the author's version of a work that was accepted for publication in Journal of Reproductive Immunology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Reproductive Immunology (97, 1, (2013) AbstractBy its genomic and non-genomic actions progesterone plays a role in preparing the endometrium for implantation and also in regulating trophoblast invasion and migration.The genomic actions of progesterone are mediated by the classical nuclear progesterone receptors, PR-A and PR-B. In addition to their genomic actions, nuclear progesterone receptors may also trigger rapid cytoplasmic signalling events. Membrane-bound progesterone receptors have been implicated in rapid non-genomic actions of progesterone.Both genomic and extra-nuclear actions of progesterone are crucial for adequate decidualization and implantation.Progesterone plays a role in establishing uterine receptivity by blocking the proliferative effect of estrogen, and by inducing genes that allow the endometrium to permit embryo attachment, and also acts as a negative regulator of trophoblast invasion by controlling matrix metalloproteinase (MMP) activity.

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Progestin inhibits and thrombin stimulates the plasminogen activator/inhibitor system in term decidual stromal cells: implications for parturition

Cited by 20 publications

References 17 publications

Role of GnRH–GnRH receptor signaling at the maternal-fetal interface

Role of GnRH–GnRH receptor signaling at the maternal-fetal interface

Comprehensive analysis of the transcriptional response of human decidual cells to lipopolysaccharide stimulation

The role of progesterone in implantation and trophoblast invasion

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