Progesterone-stimulated endometrial cell conditioned media increases in vitro produced bovine embryo blastocyst formation

Oviduct and endometrial epithelium improve in vitro produced bovine embryo developmental kinetics

“…During a recent study, we also detected an increase in IVP bovine blastocyst formation when embryos were cultured in endometrial cell-conditioned media ( Oliver et al 2023 ). The conditioned media were prepared by culturing primary bovine EEp or EF for 12 h in a standard cell culture media (Roswell Park Memorial Institute; RPMI) containing 5% FBS and 15 ng of P4.…”

“…EF) were tested for their capacity to improve in vitro cultured embryo development during coculture with some success, mostly related to blastocyst quality ( Kuzan & Wright 1982 , Voelkel et al 1985 ). More recent studies involving bovine EEp have reported increased blastocyst formation when IVP bovine embryos are cocultured with EEp monolayers or cell-conditioned media ( Sponchiado et al 2020 , Oliver et al 2023 ). During a study by Lakshmi Devi et al (2022) , coculture of IVP buffalo (Bubaline) embryos with P4 and estradiol (E2)-stimulated EEp or cell-conditioned media improved blastocyst formation and hatching.…”

“…The conditioned media were prepared by culturing primary bovine EEp or EF for 12 h in a standard cell culture media (Roswell Park Memorial Institute; RPMI) containing 5% FBS and 15 ng of P4. Culturing embryos in the EEp, EF or a combined (1:1) cell-conditioned media after day 4 of development increased blastocyst formation over nonconditioned media (control) by day 7 ( Oliver et al 2023 ). Further, the number of blastocysts within the cell-conditioned media was numerically greater than that of IVP embryos cultured in KSOM.…”

“…Further, endometrium lacking uterine glands does not support pregnancy ( Gray et al 2001 ). During a recent study, we evaluated the effects of using bovine EEp and EF to condition a standard cell culture medium to support IVP bovine embryonic development ( Oliver et al 2023 ). When compared to non-cell-conditioned medium, culturing embryos in the EEp- or EF-conditioned media from days 4 to 8 of development resulted in a greater number of blastocysts and affected blastocyst gene expression ( Oliver et al 2023 ).…”

“…During a recent study, we evaluated the effects of using bovine EEp and EF to condition a standard cell culture medium to support IVP bovine embryonic development ( Oliver et al 2023 ). When compared to non-cell-conditioned medium, culturing embryos in the EEp- or EF-conditioned media from days 4 to 8 of development resulted in a greater number of blastocysts and affected blastocyst gene expression ( Oliver et al 2023 ). Based on this information, we designed a follow-up study to investigate the impact of culturing IVP bovine embryos sequentially in a standard embryo culture medium conditioned by OEp followed by EF and or EEp using the MIRI TL6, an advanced embryo monitoring system.…”