Progesterone-Mediated Efflux of Cytosolic Chloride During the Human Sperm Acrosome Reaction

Turner, Kenneth O.; Meizel, Stanley

doi:10.1006/bbrc.1995.2197

Cited by 81 publications

(52 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The presence of three distinct CLIC proteins within the sperm head suggests a possible role for these proteins in gamete fertilization events, including spermegg membrane fusion and/or the acrosome reaction. Interestingly, a cytosolic chloride ion efflux mediated by ligand-gated chloride channels in the plasma membrane has been shown to be essential to the acrosome reaction [25][26][27]. If CLIC proteins in spermatozoa function as chloride channels, or as components or regulators of chloride channels, then it is possible that they contribute to the chloride efflux associated with the acrosome reaction.…”

Section: Discussionmentioning

confidence: 99%

Identification of chloride intracellular channel proteins in spermatozoa

et al. 2004

View full text Add to dashboard Cite

We have identified for the first time the presence of chloride intracellular channel (CLIC) proteins in bovine epididymal spermatozoa. CLIC1 was discovered during microsequencing of proteins that co-purified with protein phosphatase 1, PP1c2, in sperm extracts. In addition to CLIC1, Western blot showed that two additional CLIC family members, CLIC4 and CLIC5, are also present in spermatozoa. CLIC fusion proteins, GST-CLIC1, GST-CLIC4 and GST-CLIC5, were all able to bind to PP1c2 in sperm extracts during pull-down assays. Immunofluorescence microscopy revealed that each of the three isoforms occupies a distinct location within the cell. Given that PP1c2 is a key enzyme regulating sperm motility, PP1c2-binding proteins, such as the CLIC proteins, are likely to play significant roles in sperm function.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of chloride intracellular channel proteins in spermatozoa

et al. 2004

View full text Add to dashboard Cite

show abstract

“…In recent years, many membrane SBPs (MSBPs), characterized by a highly conserved single membranespanning domain in the N-terminal region, have been isolated and studied in animals (Falkenstein et al, 1996;Meyer et al, 1996;Cenedella et al, 1999). MSBPs were proposed to mediate high affinity binding of steroid hormones and rapid nongenomic actions Falkenstein et al, 1998;Zhu et al, 2001), such as stimulation of the Na þ /H þ exchanger (Wehling et al, 1991) and rapid cellular ion fluxes (Blackmore, 1993;Turner and Meizel, 1995;Wehling, 1997).…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis Membrane Steroid Binding Protein 1 Is Involved in Inhibition of Cell Elongation

2005

View full text Add to dashboard Cite

A putative Membrane Steroid Binding Protein (designated MSBP1) was identified and functionally characterized as a negative regulator of cell elongation in Arabidopsis thaliana. The MSBP1 gene encodes a 220-amino acid protein that can bind to progesterone, 5-dihydrotestosterone, 24-epi-brassinolide (24-eBL), and stigmasterol with different affinities in vitro. Transgenic plants overexpressing MSBP1 showed short hypocotyl phenotype and increased steroid binding capacity in membrane fractions, whereas antisense MSBP1 transgenic plants showed long hypocotyl phenotypes and reduced steroid binding capacity, indicating that MSBP1 negatively regulates hypocotyl elongation. The reduced cell elongation of MSBP1-overexpressing plants was correlated with altered expression of genes involved in cell elongation, such as expansins and extensins, indicating that enhanced MSBP1 affected a regulatory pathway for cell elongation. Suppression or overexpression of MSBP1 resulted in enhanced or reduced sensitivities, respectively, to exogenous progesterone and 24-eBL, suggesting a negative role of MSBP1 in steroid signaling. Expression of MSBP1 in hypocotyls is suppressed by darkness and activated by light, suggesting that MSBP1, as a negative regulator of cell elongation, plays a role in plant photomorphogenesis. This study demonstrates the functional roles of a steroid binding protein in growth regulation in higher plants.

show abstract

“…An important role of GABA A -regulated Cl channels is also postulated for the progesterone-induced AR (Calogero et al 1999;Turner and Meizel 1995). Thus, the chloride channel blocker picrotoxin completely inhibits the progesterone-induced AR (Calogero et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Sperm acrosome antigen-1

Breuckmann

Brucker

2002

Cell and Tissue Research

View full text Add to dashboard Cite

The acrosome reaction is a fundamental requirement for mammalian fertilization. Its exact molecular mechanisms and cellular elements are still poorly understood. We have detected an acrosomal sperm antigen, SAA-1, by monoclonal antibodies directed against SAA-1, that appears to be critically involved in the regulation of the acrosome reaction. SAA-1 is conserved within a broad range of mammalian species, emphasizing its important role in mammalian reproduction. Here we demonstrate that SAA-1 is immunohistochemically detectable in a nonmammalian vertebrate whose sperm do not possess an acrosome. In the swordtail, a live-bearing teleost with special reproductive tactics, we were able to demonstrate immunoreactivity of sperm heads of spermatids and mature sperm in the testis using monoclonal antibodies against SAA-1. Due to the cystic spermatogenesis with synchronous sperm maturation, immunoreactive maturational stages could be clearly identified. Stored immunoreactive sperm were also identified in spermathecal tissue of the female genital tract. Interestingly, immunoreactivity was furthermore detected in defined cells of the compounded endocrine organs pituitary and endocrine pancreas. All these different cell systems are involved in paracrine regulation and exhibit exocytotic properties. The possible nature of SAA-1 is discussed. Additionally, some new aspects of the morphologic composition of the swordtail pituitary are described.

show abstract

Progesterone-Mediated Efflux of Cytosolic Chloride During the Human Sperm Acrosome Reaction

Cited by 81 publications

References 0 publications

Identification of chloride intracellular channel proteins in spermatozoa

Identification of chloride intracellular channel proteins in spermatozoa

Arabidopsis Membrane Steroid Binding Protein 1 Is Involved in Inhibition of Cell Elongation

Sperm acrosome antigen-1

Contact Info

Product

Resources

About