Progesterone induces the fibulin-1 expression in human endometrial stromal cells

Nakamoto, Tetsuji; Okada, Hidetaka; Nakajima, Tatsuya; Ikuta, Akiko; Yasuda, Katsuhiko; Kanzaki, Hideharu

doi:10.1093/humrep/deh841

Cited by 31 publications

(33 citation statements)

References 42 publications

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“…These results are in agreement with earlier studies describing the repression of SDF-1 by P in breast cancer cell lines (T47D cells) and rat leiomyoma cells (ELT-3) (27,28). As previously shown, progestins enhance the expression of insulinlike growth factor binding protein 1, tissue factor, interleukin-11, interleukin-15, and fibulin-1 in ESCs, a well characterized model for progestational activity (4,24,29,30). Although most work investigating P-regulated transcription focuses on up-regulated genes, VEGF and SDF-1 repression by progestins may predict the ability to oppose the actions of E 2 in ESCs.…”

Section: Discussionsupporting

confidence: 93%

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Progestins inhibit estradiol-induced vascular endothelial growth factor and stromal cell–derived factor 1 in human endometrial stromal cells

Okada

Okamoto

Tsuzuki

et al. 2011

Fertility and Sterility

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Section: Discussionsupporting

confidence: 93%

“…Quantitative realtime PCR was performed using the Sybr Green I nucleic acid gel stain (Roche) as described previously (24). Elongation factor 1a (EF-1a) was used as reference ''housekeeping'' gene for transcription profiling.…”

Section: Rna Extraction and Real-time Pcr Analysismentioning

confidence: 99%

Progestins inhibit estradiol-induced vascular endothelial growth factor and stromal cell–derived factor 1 in human endometrial stromal cells

Okada

Okamoto

Tsuzuki

et al. 2011

Fertility and Sterility

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“…They are both highly expressed in these tissues, they accumulate within the extracellular matrix (41), and they co-localize with human SHBG within the endometrial stroma of our transgenic mice. Interactions between fibulin-1D or fibulin-2 and SHBG within the endometrium were also of particular interest because they are both up-regulated by progesterone in human and rat endometrial stromal cells (42)(43)(44), and fibulin-1D mRNA abundance fluctuates in the human endometrium in a cycle-dependent manner (44). It was therefore interesting to note these fibulin family members co-localized with human SHBG within the endometrial stroma of our transgenic mouse model and that this was most apparent with respect to the presence of immunoreactive fibulin-1 and SHBG at the basal lamina of the luminal epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

Evidence That Fibulin Family Members Contribute to the Steroid-dependent Extravascular Sequestration of Sex Hormone-binding Globulin

Catalano

Pinós

et al. 2006

Journal of Biological Chemistry

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Sex hormone-binding globulin (SHBG) binds steroids in the blood but is also present in the extravascular compartments of some tissues. Mice expressing a human SHBG transgene in the liver have human SHBG in their blood. In these animals, human SHBG accumulates within the stromal matrix of the endometrium and epididymis. This is remarkable because these tissues do not express the transgene. Human SHBG administered intravenously to wild-type mice in the presence of estradiol is rapidly sequestered within the endometrial stroma, and this prompted us to search for SHBG interacting proteins. Yeast two-hybrid screens revealed that fibulin-1D and fibulin-2 interact with the amino-terminal laminin G domain of SHBG. These interactions were verified in GST-pull down assays in which human SHBG bound the carboxyl-terminal domains of fibulin-1D and fibulin-2 in a steroid-dependent manner, with estradiol being the most effective ligand, and were enhanced by reducing the N-glycosylation of human SHBG. Like human SHBG, fibulin-1 and fibulin-2 concentrate within the endometrial stroma. In addition, SHBG co-immunoprecipitates with these fibulins in a proestrus uterine extract. These matrix-associated proteins may therefore sequester plasma SHBG within uterine stroma where it can control sex-steroid access to target cells. Given the interplay between fibulins and numerous proteins within the basal lamina, interactions between SHBG and matrix proteins may exert novel biological effects.

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“…After 2 days culture, the attached cells were fed with a steroid-reduced medium, such as RPMI-1640 medium containing 5% charcoal/ dextran-treated, heat inactivated FCS, 9 and cultured for an additional 24 h culture before reagent administration. After 24 h of steroid depletion (as day 0), cells were fed with the steroid-reduced medium that was supplemented with DHT, AG1478, or vehicle at the appropriate concentration.…”

Section: Cell Lines and Cell Culture Conditionsmentioning

confidence: 99%

Overexpression of JKTBP1 induces androgen‐independent LNCaP cell proliferation through activation of epidermal growth factor‐receptor (EGF‐R)

et al. 2008

Cell Biochemistry & Function

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Heterogenous nuclear ribonucleoprotein D-like protein (JKTBP) belongs to a new member of hnRNPs. Previous studies implied that JKTBP1 may be associated with the progression of androgen-independent (AI) prostate cancer. In this study, we generated three stable LNCaP cell lines which expressed exogenous JKTBP1. Furthermore, the effect of ectopic JKTBP1 on the proliferation of LNCaP cells and its mechanism was investigated. We originally found that the ectopic JKTBP1 expression resulted in the proliferation of LNCaP cells in an AI way, as well as inducing the upregulated expression of EGF-R and prostate-specific antigen (PSA), but did not influence the expression level of AR. Moreover, AG1478 suppressed the effect of proliferation induced by JKTBP1. In addition, immunohistochemistry showed that JKTBP1 expression was significantly elevated in AI prostate cancer tissues when compared with the androgen-dependent (AD) prostate cancer and benign prostatic hyperplasia. Our data indicated that overexpression of JKTBP1 in LNCaP cells leads to abnormal cell proliferation and may be involved in the process of AD to AI through induction of EGF-R expression.

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Progesterone induces the fibulin-1 expression in human endometrial stromal cells

Abstract: These results suggest that fiblin-1 is an important molecule that mediates progesterone action in human ESC differentiation towards implantation.

Cited by 31 publications

References 42 publications

Progestins inhibit estradiol-induced vascular endothelial growth factor and stromal cell–derived factor 1 in human endometrial stromal cells

Progestins inhibit estradiol-induced vascular endothelial growth factor and stromal cell–derived factor 1 in human endometrial stromal cells

Evidence That Fibulin Family Members Contribute to the Steroid-dependent Extravascular Sequestration of Sex Hormone-binding Globulin

Overexpression of JKTBP1 induces androgen‐independent LNCaP cell proliferation through activation of epidermal growth factor‐receptor (EGF‐R)

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